Cargando…
Treatment Response and Drug Resistance Profiling of Genotype 6 of Hepatitis C Virus in HCV/HIV Co-Infected Patients: A Pilot Study from INDIA
Hepatitis C Virus (HCV) genotype (GT) 6 demonstrates maximum genomic diversity out of all the known genotypes of HCV, attributable to its inherent intra-genotype and inter-genotype recombination property. This is the most common genotype seen in HCV/HIV co-infected cases. HIV/HCV co-infection is lin...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146731/ https://www.ncbi.nlm.nih.gov/pubmed/35632686 http://dx.doi.org/10.3390/v14050944 |
_version_ | 1784716634996342784 |
---|---|
author | Gupta, Ekta Samal, Jasmine Pandey, Amit Singh, Gaurav Gupta, Hajra A. S. Agarwal, Reshu Sharma, Manoj Kumar |
author_facet | Gupta, Ekta Samal, Jasmine Pandey, Amit Singh, Gaurav Gupta, Hajra A. S. Agarwal, Reshu Sharma, Manoj Kumar |
author_sort | Gupta, Ekta |
collection | PubMed |
description | Hepatitis C Virus (HCV) genotype (GT) 6 demonstrates maximum genomic diversity out of all the known genotypes of HCV, attributable to its inherent intra-genotype and inter-genotype recombination property. This is the most common genotype seen in HCV/HIV co-infected cases. HIV/HCV co-infection is linked with increased genetic diversity in HCV structural genes. The detailed information on the distribution of HCV GT6, its subtypes, and resistance to currently available antiviral drugs is limited in the Indian subcontinent. Therefore, in this single-center retrospective cross-sectional study, we aimed to map the occurrence of HCV GT6, its subtypes and resistance-associated substitution (RAS), and its correlation with antiviral treatment response in HCV-infected patients. From a cohort of 2052 HCV-infected patients, the overall prevalence of GT6 was 2.5% (n = 53), with a maximum of 81.1% (n = 43) seen in HCV/HIV co-infected patients. Nine different subtypes, 6a, 6b, 6f, 6i, 6n, 6u, 6v, 6w, and 6xa, were detected in the Indian population for the first time, with a predominance of 6xa (41.5%), a rare subtype, followed by 6n (39.6%). The phylogenetic analysis by the neighbor-joining method revealed three prominent viral clades, 6v, 6n, and 6xa–6u. The baseline (before treatment initiation) plasma samples of all GT6-infected patients were retrieved from −80 °C and a part of the NS5a and NS5b region of the viral genome was analyzed for the presence of RAS. No RASs were seen in the NS5b region, while in two patients (3.7%) RASs were seen at baseline in the NS5a region of the virus. Sustained viral response (SVR) was attained in 81% (n = 43) of patients. No difference in GT6 subtype distribution or occurrence of RAS was seen between mono-infected HCV and HIV/HCV co-infected cases. Our study revealed that RAS at baseline did not influence the attainment of SVR and the currently available antiviral therapy is effective against GT6 mono-infected and HIV/HCV co-infected patients. |
format | Online Article Text |
id | pubmed-9146731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91467312022-05-29 Treatment Response and Drug Resistance Profiling of Genotype 6 of Hepatitis C Virus in HCV/HIV Co-Infected Patients: A Pilot Study from INDIA Gupta, Ekta Samal, Jasmine Pandey, Amit Singh, Gaurav Gupta, Hajra A. S. Agarwal, Reshu Sharma, Manoj Kumar Viruses Communication Hepatitis C Virus (HCV) genotype (GT) 6 demonstrates maximum genomic diversity out of all the known genotypes of HCV, attributable to its inherent intra-genotype and inter-genotype recombination property. This is the most common genotype seen in HCV/HIV co-infected cases. HIV/HCV co-infection is linked with increased genetic diversity in HCV structural genes. The detailed information on the distribution of HCV GT6, its subtypes, and resistance to currently available antiviral drugs is limited in the Indian subcontinent. Therefore, in this single-center retrospective cross-sectional study, we aimed to map the occurrence of HCV GT6, its subtypes and resistance-associated substitution (RAS), and its correlation with antiviral treatment response in HCV-infected patients. From a cohort of 2052 HCV-infected patients, the overall prevalence of GT6 was 2.5% (n = 53), with a maximum of 81.1% (n = 43) seen in HCV/HIV co-infected patients. Nine different subtypes, 6a, 6b, 6f, 6i, 6n, 6u, 6v, 6w, and 6xa, were detected in the Indian population for the first time, with a predominance of 6xa (41.5%), a rare subtype, followed by 6n (39.6%). The phylogenetic analysis by the neighbor-joining method revealed three prominent viral clades, 6v, 6n, and 6xa–6u. The baseline (before treatment initiation) plasma samples of all GT6-infected patients were retrieved from −80 °C and a part of the NS5a and NS5b region of the viral genome was analyzed for the presence of RAS. No RASs were seen in the NS5b region, while in two patients (3.7%) RASs were seen at baseline in the NS5a region of the virus. Sustained viral response (SVR) was attained in 81% (n = 43) of patients. No difference in GT6 subtype distribution or occurrence of RAS was seen between mono-infected HCV and HIV/HCV co-infected cases. Our study revealed that RAS at baseline did not influence the attainment of SVR and the currently available antiviral therapy is effective against GT6 mono-infected and HIV/HCV co-infected patients. MDPI 2022-04-30 /pmc/articles/PMC9146731/ /pubmed/35632686 http://dx.doi.org/10.3390/v14050944 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Gupta, Ekta Samal, Jasmine Pandey, Amit Singh, Gaurav Gupta, Hajra A. S. Agarwal, Reshu Sharma, Manoj Kumar Treatment Response and Drug Resistance Profiling of Genotype 6 of Hepatitis C Virus in HCV/HIV Co-Infected Patients: A Pilot Study from INDIA |
title | Treatment Response and Drug Resistance Profiling of Genotype 6 of Hepatitis C Virus in HCV/HIV Co-Infected Patients: A Pilot Study from INDIA |
title_full | Treatment Response and Drug Resistance Profiling of Genotype 6 of Hepatitis C Virus in HCV/HIV Co-Infected Patients: A Pilot Study from INDIA |
title_fullStr | Treatment Response and Drug Resistance Profiling of Genotype 6 of Hepatitis C Virus in HCV/HIV Co-Infected Patients: A Pilot Study from INDIA |
title_full_unstemmed | Treatment Response and Drug Resistance Profiling of Genotype 6 of Hepatitis C Virus in HCV/HIV Co-Infected Patients: A Pilot Study from INDIA |
title_short | Treatment Response and Drug Resistance Profiling of Genotype 6 of Hepatitis C Virus in HCV/HIV Co-Infected Patients: A Pilot Study from INDIA |
title_sort | treatment response and drug resistance profiling of genotype 6 of hepatitis c virus in hcv/hiv co-infected patients: a pilot study from india |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146731/ https://www.ncbi.nlm.nih.gov/pubmed/35632686 http://dx.doi.org/10.3390/v14050944 |
work_keys_str_mv | AT guptaekta treatmentresponseanddrugresistanceprofilingofgenotype6ofhepatitiscvirusinhcvhivcoinfectedpatientsapilotstudyfromindia AT samaljasmine treatmentresponseanddrugresistanceprofilingofgenotype6ofhepatitiscvirusinhcvhivcoinfectedpatientsapilotstudyfromindia AT pandeyamit treatmentresponseanddrugresistanceprofilingofgenotype6ofhepatitiscvirusinhcvhivcoinfectedpatientsapilotstudyfromindia AT singhgaurav treatmentresponseanddrugresistanceprofilingofgenotype6ofhepatitiscvirusinhcvhivcoinfectedpatientsapilotstudyfromindia AT guptahajraas treatmentresponseanddrugresistanceprofilingofgenotype6ofhepatitiscvirusinhcvhivcoinfectedpatientsapilotstudyfromindia AT agarwalreshu treatmentresponseanddrugresistanceprofilingofgenotype6ofhepatitiscvirusinhcvhivcoinfectedpatientsapilotstudyfromindia AT sharmamanojkumar treatmentresponseanddrugresistanceprofilingofgenotype6ofhepatitiscvirusinhcvhivcoinfectedpatientsapilotstudyfromindia |