Cargando…

Anti-Inflammatory Effects of Mitrephora sirikitiae Leaf Extract and Isolated Lignans in RAW 264.7 Cells

Mitrephora sirikitiae Weeras., Chalermglin & R.M.K. Saunders has been reported as a rich source of lignans that contribute to biological activities and health benefits. However, cellular anti-inflammatory effects of M. sirikitiae leaves and their lignan compounds have not been fully elucidated....

Descripción completa

Detalles Bibliográficos
Autores principales: Mangmool, Supachoke, Limpichai, Chayaporn, Han, Khine Kyi, Reutrakul, Vichai, Anantachoke, Natthinee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147141/
https://www.ncbi.nlm.nih.gov/pubmed/35630789
http://dx.doi.org/10.3390/molecules27103313
_version_ 1784716735141642240
author Mangmool, Supachoke
Limpichai, Chayaporn
Han, Khine Kyi
Reutrakul, Vichai
Anantachoke, Natthinee
author_facet Mangmool, Supachoke
Limpichai, Chayaporn
Han, Khine Kyi
Reutrakul, Vichai
Anantachoke, Natthinee
author_sort Mangmool, Supachoke
collection PubMed
description Mitrephora sirikitiae Weeras., Chalermglin & R.M.K. Saunders has been reported as a rich source of lignans that contribute to biological activities and health benefits. However, cellular anti-inflammatory effects of M. sirikitiae leaves and their lignan compounds have not been fully elucidated. Therefore, this study aimed to investigate the anti-inflammatory activities of methanol extract of M. sirikitiae leaves and their lignan constituents on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 mouse macrophage cells. Treatment of RAW 264.7 cells with the methanol extract of M. sirikitiae leaves and its isolated lignans, including (−)-phylligenin (2) and 3′,4-O-dimethylcedrusin (6) significantly decreased LPS-induced prostaglandin E(2) (PGE(2)) and nitric oxide (NO) productions. These inhibitory effects of the extract and isolated lignans on LPS-induced upregulation of PGE(2) and NO productions were derived from the suppression of cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) production, respectively. In addition, treatment with 2-(3,4-dimethoxyphenyl)-6-(3,5-dimethoxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (3) and mitrephoran (5) was able to suppress LPS-induced tumor necrosis factor alpha (TNF-α) secretion and synthesis in RAW 264.7 cells. These results demonstrated that M. sirikitiae leaves and some isolated lignans exhibited potent anti-inflammatory activity through the inhibition of secretion and synthesis of PGE(2), NO, and TNF-α.
format Online
Article
Text
id pubmed-9147141
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91471412022-05-29 Anti-Inflammatory Effects of Mitrephora sirikitiae Leaf Extract and Isolated Lignans in RAW 264.7 Cells Mangmool, Supachoke Limpichai, Chayaporn Han, Khine Kyi Reutrakul, Vichai Anantachoke, Natthinee Molecules Article Mitrephora sirikitiae Weeras., Chalermglin & R.M.K. Saunders has been reported as a rich source of lignans that contribute to biological activities and health benefits. However, cellular anti-inflammatory effects of M. sirikitiae leaves and their lignan compounds have not been fully elucidated. Therefore, this study aimed to investigate the anti-inflammatory activities of methanol extract of M. sirikitiae leaves and their lignan constituents on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 mouse macrophage cells. Treatment of RAW 264.7 cells with the methanol extract of M. sirikitiae leaves and its isolated lignans, including (−)-phylligenin (2) and 3′,4-O-dimethylcedrusin (6) significantly decreased LPS-induced prostaglandin E(2) (PGE(2)) and nitric oxide (NO) productions. These inhibitory effects of the extract and isolated lignans on LPS-induced upregulation of PGE(2) and NO productions were derived from the suppression of cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) production, respectively. In addition, treatment with 2-(3,4-dimethoxyphenyl)-6-(3,5-dimethoxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (3) and mitrephoran (5) was able to suppress LPS-induced tumor necrosis factor alpha (TNF-α) secretion and synthesis in RAW 264.7 cells. These results demonstrated that M. sirikitiae leaves and some isolated lignans exhibited potent anti-inflammatory activity through the inhibition of secretion and synthesis of PGE(2), NO, and TNF-α. MDPI 2022-05-21 /pmc/articles/PMC9147141/ /pubmed/35630789 http://dx.doi.org/10.3390/molecules27103313 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mangmool, Supachoke
Limpichai, Chayaporn
Han, Khine Kyi
Reutrakul, Vichai
Anantachoke, Natthinee
Anti-Inflammatory Effects of Mitrephora sirikitiae Leaf Extract and Isolated Lignans in RAW 264.7 Cells
title Anti-Inflammatory Effects of Mitrephora sirikitiae Leaf Extract and Isolated Lignans in RAW 264.7 Cells
title_full Anti-Inflammatory Effects of Mitrephora sirikitiae Leaf Extract and Isolated Lignans in RAW 264.7 Cells
title_fullStr Anti-Inflammatory Effects of Mitrephora sirikitiae Leaf Extract and Isolated Lignans in RAW 264.7 Cells
title_full_unstemmed Anti-Inflammatory Effects of Mitrephora sirikitiae Leaf Extract and Isolated Lignans in RAW 264.7 Cells
title_short Anti-Inflammatory Effects of Mitrephora sirikitiae Leaf Extract and Isolated Lignans in RAW 264.7 Cells
title_sort anti-inflammatory effects of mitrephora sirikitiae leaf extract and isolated lignans in raw 264.7 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147141/
https://www.ncbi.nlm.nih.gov/pubmed/35630789
http://dx.doi.org/10.3390/molecules27103313
work_keys_str_mv AT mangmoolsupachoke antiinflammatoryeffectsofmitrephorasirikitiaeleafextractandisolatedlignansinraw2647cells
AT limpichaichayaporn antiinflammatoryeffectsofmitrephorasirikitiaeleafextractandisolatedlignansinraw2647cells
AT hankhinekyi antiinflammatoryeffectsofmitrephorasirikitiaeleafextractandisolatedlignansinraw2647cells
AT reutrakulvichai antiinflammatoryeffectsofmitrephorasirikitiaeleafextractandisolatedlignansinraw2647cells
AT anantachokenatthinee antiinflammatoryeffectsofmitrephorasirikitiaeleafextractandisolatedlignansinraw2647cells