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A Vaccine Construction against COVID-19-Associated Mucormycosis Contrived with Immunoinformatics-Based Scavenging of Potential Mucoralean Epitopes

Mucormycosis is a group of infections, caused by multiple fungal species, which affect many human organs and is lethal in immunocompromised patients. During the COVID-19 pandemic, the current wave of mucormycosis is a challenge to medical professionals as its effects are multiplied because of the se...

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Autores principales: Naveed, Muhammad, Ali, Urooj, Karobari, Mohmed Isaqali, Ahmed, Naveed, Mohamed, Roshan Noor, Abullais, Shahabe Saquib, Kader, Mohammed Abdul, Marya, Anand, Messina, Pietro, Scardina, Giuseppe Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147184/
https://www.ncbi.nlm.nih.gov/pubmed/35632420
http://dx.doi.org/10.3390/vaccines10050664
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author Naveed, Muhammad
Ali, Urooj
Karobari, Mohmed Isaqali
Ahmed, Naveed
Mohamed, Roshan Noor
Abullais, Shahabe Saquib
Kader, Mohammed Abdul
Marya, Anand
Messina, Pietro
Scardina, Giuseppe Alessandro
author_facet Naveed, Muhammad
Ali, Urooj
Karobari, Mohmed Isaqali
Ahmed, Naveed
Mohamed, Roshan Noor
Abullais, Shahabe Saquib
Kader, Mohammed Abdul
Marya, Anand
Messina, Pietro
Scardina, Giuseppe Alessandro
author_sort Naveed, Muhammad
collection PubMed
description Mucormycosis is a group of infections, caused by multiple fungal species, which affect many human organs and is lethal in immunocompromised patients. During the COVID-19 pandemic, the current wave of mucormycosis is a challenge to medical professionals as its effects are multiplied because of the severity of COVID-19 infection. The variant of concern, Omicron, has been linked to fatal mucormycosis infections in the US and Asia. Consequently, current postdiagnostic treatments of mucormycosis have been rendered unsatisfactory. In this hour of need, a preinfection cure is needed that may prevent lethal infections in immunocompromised individuals. This study proposes a potential vaccine construct targeting mucor and rhizopus species responsible for mucormycosis infections, providing immunoprotection to immunocompromised patients. The vaccine construct, with an antigenicity score of 0.75 covering, on average, 92–98% of the world population, was designed using an immunoinformatics approach. Molecular interactions with major histocompatibility complex-1 (MHC-I), Toll-like receptors-2 (TLR2), and glucose-regulated protein 78 (GRP78), with scores of −896.0, −948.4, and −925.0, respectively, demonstrated its potential to bind with the human immune receptors. It elicited a strong predicted innate and adaptive immune response in the form of helper T (Th) cells, cytotoxic T (TC) cells, B cells, natural killer (NK) cells, and macrophages. The vaccine cloned in the pBR322 vector showed positive amplification, further solidifying its stability and potential. The proposed construct holds a promising approach as the first step towards an antimucormycosis vaccine and may contribute to minimizing postdiagnostic burdens and failures.
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spelling pubmed-91471842022-05-29 A Vaccine Construction against COVID-19-Associated Mucormycosis Contrived with Immunoinformatics-Based Scavenging of Potential Mucoralean Epitopes Naveed, Muhammad Ali, Urooj Karobari, Mohmed Isaqali Ahmed, Naveed Mohamed, Roshan Noor Abullais, Shahabe Saquib Kader, Mohammed Abdul Marya, Anand Messina, Pietro Scardina, Giuseppe Alessandro Vaccines (Basel) Article Mucormycosis is a group of infections, caused by multiple fungal species, which affect many human organs and is lethal in immunocompromised patients. During the COVID-19 pandemic, the current wave of mucormycosis is a challenge to medical professionals as its effects are multiplied because of the severity of COVID-19 infection. The variant of concern, Omicron, has been linked to fatal mucormycosis infections in the US and Asia. Consequently, current postdiagnostic treatments of mucormycosis have been rendered unsatisfactory. In this hour of need, a preinfection cure is needed that may prevent lethal infections in immunocompromised individuals. This study proposes a potential vaccine construct targeting mucor and rhizopus species responsible for mucormycosis infections, providing immunoprotection to immunocompromised patients. The vaccine construct, with an antigenicity score of 0.75 covering, on average, 92–98% of the world population, was designed using an immunoinformatics approach. Molecular interactions with major histocompatibility complex-1 (MHC-I), Toll-like receptors-2 (TLR2), and glucose-regulated protein 78 (GRP78), with scores of −896.0, −948.4, and −925.0, respectively, demonstrated its potential to bind with the human immune receptors. It elicited a strong predicted innate and adaptive immune response in the form of helper T (Th) cells, cytotoxic T (TC) cells, B cells, natural killer (NK) cells, and macrophages. The vaccine cloned in the pBR322 vector showed positive amplification, further solidifying its stability and potential. The proposed construct holds a promising approach as the first step towards an antimucormycosis vaccine and may contribute to minimizing postdiagnostic burdens and failures. MDPI 2022-04-22 /pmc/articles/PMC9147184/ /pubmed/35632420 http://dx.doi.org/10.3390/vaccines10050664 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Naveed, Muhammad
Ali, Urooj
Karobari, Mohmed Isaqali
Ahmed, Naveed
Mohamed, Roshan Noor
Abullais, Shahabe Saquib
Kader, Mohammed Abdul
Marya, Anand
Messina, Pietro
Scardina, Giuseppe Alessandro
A Vaccine Construction against COVID-19-Associated Mucormycosis Contrived with Immunoinformatics-Based Scavenging of Potential Mucoralean Epitopes
title A Vaccine Construction against COVID-19-Associated Mucormycosis Contrived with Immunoinformatics-Based Scavenging of Potential Mucoralean Epitopes
title_full A Vaccine Construction against COVID-19-Associated Mucormycosis Contrived with Immunoinformatics-Based Scavenging of Potential Mucoralean Epitopes
title_fullStr A Vaccine Construction against COVID-19-Associated Mucormycosis Contrived with Immunoinformatics-Based Scavenging of Potential Mucoralean Epitopes
title_full_unstemmed A Vaccine Construction against COVID-19-Associated Mucormycosis Contrived with Immunoinformatics-Based Scavenging of Potential Mucoralean Epitopes
title_short A Vaccine Construction against COVID-19-Associated Mucormycosis Contrived with Immunoinformatics-Based Scavenging of Potential Mucoralean Epitopes
title_sort vaccine construction against covid-19-associated mucormycosis contrived with immunoinformatics-based scavenging of potential mucoralean epitopes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147184/
https://www.ncbi.nlm.nih.gov/pubmed/35632420
http://dx.doi.org/10.3390/vaccines10050664
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