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Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle
Chinese Yellow Cattle, an ancient and domesticated breed for draft service, provide unique animal genetic resources with excellent genetic features, including crude feed tolerance, good stress resistance, strong adaptability, and tender meat quality; however, their production performance and meat yi...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147296/ https://www.ncbi.nlm.nih.gov/pubmed/35629295 http://dx.doi.org/10.3390/life12050627 |
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author | Zhao, Yuefang Yang, Lei Su, Guanghua Wei, Zhuying Liu, Xuefei Song, Lishuang Hai, Chao Wu, Di Hao, Zhenting Wu, Yunxi Zhang, Li Bai, Chunling Li, Guangpeng |
author_facet | Zhao, Yuefang Yang, Lei Su, Guanghua Wei, Zhuying Liu, Xuefei Song, Lishuang Hai, Chao Wu, Di Hao, Zhenting Wu, Yunxi Zhang, Li Bai, Chunling Li, Guangpeng |
author_sort | Zhao, Yuefang |
collection | PubMed |
description | Chinese Yellow Cattle, an ancient and domesticated breed for draft service, provide unique animal genetic resources with excellent genetic features, including crude feed tolerance, good stress resistance, strong adaptability, and tender meat quality; however, their production performance and meat yield are significantly inferior. Herein, the myostatin gene (MSTN), a negative regulator of skeletal muscle development, was knocked out by CRISPR/Cas9 technology. Eight MSTN gene-edited bull calves (MT) were born, and six of them are well-developed. Compared with the control cattle (WT), the growth trait indexes of MT cattle were generally increased, and the hindquarters especially were significantly improved. The biochemical indexes and the semen characteristics demonstrated that MT bulls were healthy and fertile. Consistent with our conjecture, the wobble and beating of MT bull spermatozoa were significantly higher than that of WT. Nine sperm motility-related proteins and nineteen mitochondrial-related proteins were identified by up-regulation in MT bull spermatozoa using FLQ proteomic technique and act to govern sperm flagellum assembly, organization, and beating and provide sufficient energy for sperm motility. The current study confirmed that the MSTN gene-edited Chinese Yellow cattle have improved growth traits and normal fertility, which can be used for beef cattle production and breeding. |
format | Online Article Text |
id | pubmed-9147296 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91472962022-05-29 Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle Zhao, Yuefang Yang, Lei Su, Guanghua Wei, Zhuying Liu, Xuefei Song, Lishuang Hai, Chao Wu, Di Hao, Zhenting Wu, Yunxi Zhang, Li Bai, Chunling Li, Guangpeng Life (Basel) Article Chinese Yellow Cattle, an ancient and domesticated breed for draft service, provide unique animal genetic resources with excellent genetic features, including crude feed tolerance, good stress resistance, strong adaptability, and tender meat quality; however, their production performance and meat yield are significantly inferior. Herein, the myostatin gene (MSTN), a negative regulator of skeletal muscle development, was knocked out by CRISPR/Cas9 technology. Eight MSTN gene-edited bull calves (MT) were born, and six of them are well-developed. Compared with the control cattle (WT), the growth trait indexes of MT cattle were generally increased, and the hindquarters especially were significantly improved. The biochemical indexes and the semen characteristics demonstrated that MT bulls were healthy and fertile. Consistent with our conjecture, the wobble and beating of MT bull spermatozoa were significantly higher than that of WT. Nine sperm motility-related proteins and nineteen mitochondrial-related proteins were identified by up-regulation in MT bull spermatozoa using FLQ proteomic technique and act to govern sperm flagellum assembly, organization, and beating and provide sufficient energy for sperm motility. The current study confirmed that the MSTN gene-edited Chinese Yellow cattle have improved growth traits and normal fertility, which can be used for beef cattle production and breeding. MDPI 2022-04-23 /pmc/articles/PMC9147296/ /pubmed/35629295 http://dx.doi.org/10.3390/life12050627 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Yuefang Yang, Lei Su, Guanghua Wei, Zhuying Liu, Xuefei Song, Lishuang Hai, Chao Wu, Di Hao, Zhenting Wu, Yunxi Zhang, Li Bai, Chunling Li, Guangpeng Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle |
title | Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle |
title_full | Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle |
title_fullStr | Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle |
title_full_unstemmed | Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle |
title_short | Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle |
title_sort | growth traits and sperm proteomics analyses of myostatin gene-edited chinese yellow cattle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147296/ https://www.ncbi.nlm.nih.gov/pubmed/35629295 http://dx.doi.org/10.3390/life12050627 |
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