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Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle

Chinese Yellow Cattle, an ancient and domesticated breed for draft service, provide unique animal genetic resources with excellent genetic features, including crude feed tolerance, good stress resistance, strong adaptability, and tender meat quality; however, their production performance and meat yi...

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Autores principales: Zhao, Yuefang, Yang, Lei, Su, Guanghua, Wei, Zhuying, Liu, Xuefei, Song, Lishuang, Hai, Chao, Wu, Di, Hao, Zhenting, Wu, Yunxi, Zhang, Li, Bai, Chunling, Li, Guangpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147296/
https://www.ncbi.nlm.nih.gov/pubmed/35629295
http://dx.doi.org/10.3390/life12050627
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author Zhao, Yuefang
Yang, Lei
Su, Guanghua
Wei, Zhuying
Liu, Xuefei
Song, Lishuang
Hai, Chao
Wu, Di
Hao, Zhenting
Wu, Yunxi
Zhang, Li
Bai, Chunling
Li, Guangpeng
author_facet Zhao, Yuefang
Yang, Lei
Su, Guanghua
Wei, Zhuying
Liu, Xuefei
Song, Lishuang
Hai, Chao
Wu, Di
Hao, Zhenting
Wu, Yunxi
Zhang, Li
Bai, Chunling
Li, Guangpeng
author_sort Zhao, Yuefang
collection PubMed
description Chinese Yellow Cattle, an ancient and domesticated breed for draft service, provide unique animal genetic resources with excellent genetic features, including crude feed tolerance, good stress resistance, strong adaptability, and tender meat quality; however, their production performance and meat yield are significantly inferior. Herein, the myostatin gene (MSTN), a negative regulator of skeletal muscle development, was knocked out by CRISPR/Cas9 technology. Eight MSTN gene-edited bull calves (MT) were born, and six of them are well-developed. Compared with the control cattle (WT), the growth trait indexes of MT cattle were generally increased, and the hindquarters especially were significantly improved. The biochemical indexes and the semen characteristics demonstrated that MT bulls were healthy and fertile. Consistent with our conjecture, the wobble and beating of MT bull spermatozoa were significantly higher than that of WT. Nine sperm motility-related proteins and nineteen mitochondrial-related proteins were identified by up-regulation in MT bull spermatozoa using FLQ proteomic technique and act to govern sperm flagellum assembly, organization, and beating and provide sufficient energy for sperm motility. The current study confirmed that the MSTN gene-edited Chinese Yellow cattle have improved growth traits and normal fertility, which can be used for beef cattle production and breeding.
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spelling pubmed-91472962022-05-29 Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle Zhao, Yuefang Yang, Lei Su, Guanghua Wei, Zhuying Liu, Xuefei Song, Lishuang Hai, Chao Wu, Di Hao, Zhenting Wu, Yunxi Zhang, Li Bai, Chunling Li, Guangpeng Life (Basel) Article Chinese Yellow Cattle, an ancient and domesticated breed for draft service, provide unique animal genetic resources with excellent genetic features, including crude feed tolerance, good stress resistance, strong adaptability, and tender meat quality; however, their production performance and meat yield are significantly inferior. Herein, the myostatin gene (MSTN), a negative regulator of skeletal muscle development, was knocked out by CRISPR/Cas9 technology. Eight MSTN gene-edited bull calves (MT) were born, and six of them are well-developed. Compared with the control cattle (WT), the growth trait indexes of MT cattle were generally increased, and the hindquarters especially were significantly improved. The biochemical indexes and the semen characteristics demonstrated that MT bulls were healthy and fertile. Consistent with our conjecture, the wobble and beating of MT bull spermatozoa were significantly higher than that of WT. Nine sperm motility-related proteins and nineteen mitochondrial-related proteins were identified by up-regulation in MT bull spermatozoa using FLQ proteomic technique and act to govern sperm flagellum assembly, organization, and beating and provide sufficient energy for sperm motility. The current study confirmed that the MSTN gene-edited Chinese Yellow cattle have improved growth traits and normal fertility, which can be used for beef cattle production and breeding. MDPI 2022-04-23 /pmc/articles/PMC9147296/ /pubmed/35629295 http://dx.doi.org/10.3390/life12050627 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Yuefang
Yang, Lei
Su, Guanghua
Wei, Zhuying
Liu, Xuefei
Song, Lishuang
Hai, Chao
Wu, Di
Hao, Zhenting
Wu, Yunxi
Zhang, Li
Bai, Chunling
Li, Guangpeng
Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle
title Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle
title_full Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle
title_fullStr Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle
title_full_unstemmed Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle
title_short Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle
title_sort growth traits and sperm proteomics analyses of myostatin gene-edited chinese yellow cattle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147296/
https://www.ncbi.nlm.nih.gov/pubmed/35629295
http://dx.doi.org/10.3390/life12050627
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