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A Collection of Melon (Cucumis melo) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation

At times of fruit skin failure, reticulation made of a wound-periderm is formed below the cracked skin in order to seal the damaged tissue. Preceding investigations shed light on the mechanisms underlying the formation of fruit skin reticulation, demonstrating that the walls of periderm cells are he...

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Autores principales: Manasherova, Ekaterina, Cohen, Hagai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147331/
https://www.ncbi.nlm.nih.gov/pubmed/35631761
http://dx.doi.org/10.3390/plants11101336
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author Manasherova, Ekaterina
Cohen, Hagai
author_facet Manasherova, Ekaterina
Cohen, Hagai
author_sort Manasherova, Ekaterina
collection PubMed
description At times of fruit skin failure, reticulation made of a wound-periderm is formed below the cracked skin in order to seal the damaged tissue. Preceding investigations shed light on the mechanisms underlying the formation of fruit skin reticulation, demonstrating that the walls of periderm cells are heavily suberized and lignified. However, the relative contribution of the suberin pathway to these processes, as well as the association between suberin contents in the periderm tissue and reticulation degree, are largely unknown. To strengthen our understanding on these important physiological and agricultural aspects, we comparatively profiled skin tissues of a collection of smooth- and reticulated-skin melon (Cucumis melo) cultivars for suberin monomer composition via gas chromatography-mass spectrometry (GC-MS). This metabolite profiling approach accompanied by statistical tools highlighted the fundamental chemical differences between the skin of smooth fruit made of a typical cuticle, to the skin of reticulated fruit made of large amounts of archetypal suberin building blocks including hydroxycinnamic acids, very long chain fatty acids, fatty alcohols, α-hydroxyacids, ω-hydroxyacids, and α,ω-diacids. Next, using image analysis we generated ‘reticulation maps’ and calculated the relative densities of reticulation. We then performed correlation assays in order to monitor suberin monomers that specifically correlate well with reticulation degree. Nonetheless, total suberin contents and most suberin building blocks did not show high correlations with reticulation degree, further suggesting that additional factors are likely to influence and regulate these processes. Altogether, the data provided vital information regarding the relative contribution of the suberin pathway to periderm formation and skin reticulation.
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spelling pubmed-91473312022-05-29 A Collection of Melon (Cucumis melo) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation Manasherova, Ekaterina Cohen, Hagai Plants (Basel) Article At times of fruit skin failure, reticulation made of a wound-periderm is formed below the cracked skin in order to seal the damaged tissue. Preceding investigations shed light on the mechanisms underlying the formation of fruit skin reticulation, demonstrating that the walls of periderm cells are heavily suberized and lignified. However, the relative contribution of the suberin pathway to these processes, as well as the association between suberin contents in the periderm tissue and reticulation degree, are largely unknown. To strengthen our understanding on these important physiological and agricultural aspects, we comparatively profiled skin tissues of a collection of smooth- and reticulated-skin melon (Cucumis melo) cultivars for suberin monomer composition via gas chromatography-mass spectrometry (GC-MS). This metabolite profiling approach accompanied by statistical tools highlighted the fundamental chemical differences between the skin of smooth fruit made of a typical cuticle, to the skin of reticulated fruit made of large amounts of archetypal suberin building blocks including hydroxycinnamic acids, very long chain fatty acids, fatty alcohols, α-hydroxyacids, ω-hydroxyacids, and α,ω-diacids. Next, using image analysis we generated ‘reticulation maps’ and calculated the relative densities of reticulation. We then performed correlation assays in order to monitor suberin monomers that specifically correlate well with reticulation degree. Nonetheless, total suberin contents and most suberin building blocks did not show high correlations with reticulation degree, further suggesting that additional factors are likely to influence and regulate these processes. Altogether, the data provided vital information regarding the relative contribution of the suberin pathway to periderm formation and skin reticulation. MDPI 2022-05-18 /pmc/articles/PMC9147331/ /pubmed/35631761 http://dx.doi.org/10.3390/plants11101336 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Manasherova, Ekaterina
Cohen, Hagai
A Collection of Melon (Cucumis melo) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation
title A Collection of Melon (Cucumis melo) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation
title_full A Collection of Melon (Cucumis melo) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation
title_fullStr A Collection of Melon (Cucumis melo) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation
title_full_unstemmed A Collection of Melon (Cucumis melo) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation
title_short A Collection of Melon (Cucumis melo) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation
title_sort collection of melon (cucumis melo) fruit cultivars with varied skin appearances provide insight to the contribution of suberin in periderm formation and reticulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147331/
https://www.ncbi.nlm.nih.gov/pubmed/35631761
http://dx.doi.org/10.3390/plants11101336
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