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Evaluation of TILI-2 as an Anti-Tyrosinase, Anti-Oxidative Agent and Its Role in Preventing Melanogenesis Using a Proteomics Approach

There is a desire to develop new molecules that can combat hyperpigmentation. To this end, the N-terminal cysteine-containing heptapeptide TILI-2 has shown promising preliminary results. In this work, the mechanism by which it works was evaluated using a series of biochemical assays focusing on know...

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Autores principales: Joompang, Anupong, Anwised, Preeyanan, Klaynongsruang, Sompong, Roytrakul, Sittiruk, Taemaitree, Lapatrada, Jangpromma, Nisachon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147390/
https://www.ncbi.nlm.nih.gov/pubmed/35630706
http://dx.doi.org/10.3390/molecules27103228
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author Joompang, Anupong
Anwised, Preeyanan
Klaynongsruang, Sompong
Roytrakul, Sittiruk
Taemaitree, Lapatrada
Jangpromma, Nisachon
author_facet Joompang, Anupong
Anwised, Preeyanan
Klaynongsruang, Sompong
Roytrakul, Sittiruk
Taemaitree, Lapatrada
Jangpromma, Nisachon
author_sort Joompang, Anupong
collection PubMed
description There is a desire to develop new molecules that can combat hyperpigmentation. To this end, the N-terminal cysteine-containing heptapeptide TILI-2 has shown promising preliminary results. In this work, the mechanism by which it works was evaluated using a series of biochemical assays focusing on known biochemical pathways, followed by LC-MS/MS proteomics to discover pathways that have not been considered before. We demonstrate that TILI-2 is a competitive inhibitor of tyrosinase’s monophenolase activity and it could potentially scavenge ABTS and DPPH radicals. It has a very low cytotoxicity up to 1400 µM against human fibroblast NFDH cells and macrophage-like RAW 264.7 cells. Our proteomics study revealed that another putative mechanism by which TILI-2 may reduce melanin production involves the disruption of the TGF-β signaling pathway in mouse B16F1 cells. This result suggests that TILI-2 has potential scope to be used as a depigmenting agent.
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spelling pubmed-91473902022-05-29 Evaluation of TILI-2 as an Anti-Tyrosinase, Anti-Oxidative Agent and Its Role in Preventing Melanogenesis Using a Proteomics Approach Joompang, Anupong Anwised, Preeyanan Klaynongsruang, Sompong Roytrakul, Sittiruk Taemaitree, Lapatrada Jangpromma, Nisachon Molecules Article There is a desire to develop new molecules that can combat hyperpigmentation. To this end, the N-terminal cysteine-containing heptapeptide TILI-2 has shown promising preliminary results. In this work, the mechanism by which it works was evaluated using a series of biochemical assays focusing on known biochemical pathways, followed by LC-MS/MS proteomics to discover pathways that have not been considered before. We demonstrate that TILI-2 is a competitive inhibitor of tyrosinase’s monophenolase activity and it could potentially scavenge ABTS and DPPH radicals. It has a very low cytotoxicity up to 1400 µM against human fibroblast NFDH cells and macrophage-like RAW 264.7 cells. Our proteomics study revealed that another putative mechanism by which TILI-2 may reduce melanin production involves the disruption of the TGF-β signaling pathway in mouse B16F1 cells. This result suggests that TILI-2 has potential scope to be used as a depigmenting agent. MDPI 2022-05-18 /pmc/articles/PMC9147390/ /pubmed/35630706 http://dx.doi.org/10.3390/molecules27103228 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Joompang, Anupong
Anwised, Preeyanan
Klaynongsruang, Sompong
Roytrakul, Sittiruk
Taemaitree, Lapatrada
Jangpromma, Nisachon
Evaluation of TILI-2 as an Anti-Tyrosinase, Anti-Oxidative Agent and Its Role in Preventing Melanogenesis Using a Proteomics Approach
title Evaluation of TILI-2 as an Anti-Tyrosinase, Anti-Oxidative Agent and Its Role in Preventing Melanogenesis Using a Proteomics Approach
title_full Evaluation of TILI-2 as an Anti-Tyrosinase, Anti-Oxidative Agent and Its Role in Preventing Melanogenesis Using a Proteomics Approach
title_fullStr Evaluation of TILI-2 as an Anti-Tyrosinase, Anti-Oxidative Agent and Its Role in Preventing Melanogenesis Using a Proteomics Approach
title_full_unstemmed Evaluation of TILI-2 as an Anti-Tyrosinase, Anti-Oxidative Agent and Its Role in Preventing Melanogenesis Using a Proteomics Approach
title_short Evaluation of TILI-2 as an Anti-Tyrosinase, Anti-Oxidative Agent and Its Role in Preventing Melanogenesis Using a Proteomics Approach
title_sort evaluation of tili-2 as an anti-tyrosinase, anti-oxidative agent and its role in preventing melanogenesis using a proteomics approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147390/
https://www.ncbi.nlm.nih.gov/pubmed/35630706
http://dx.doi.org/10.3390/molecules27103228
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