Cargando…
A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats
Denture stomatitis (DS) is a common infection in denture wearers, especially women. This study evaluated the induction of DS using acrylic devices attached to the palate of rats combined with inoculation of Candida spp. Immunocompetent male and female rats received a carbohydrate-rich diet. Impressi...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147495/ https://www.ncbi.nlm.nih.gov/pubmed/35628722 http://dx.doi.org/10.3390/jof8050466 |
_version_ | 1784716821445738496 |
---|---|
author | Sakima, Vinicius Tatsuyuji Vega-Chacón, Yuliana Cerri, Paulo Sergio Shokeen, Bhumika Lux, Renate Mima, Ewerton Garcia de Oliveira |
author_facet | Sakima, Vinicius Tatsuyuji Vega-Chacón, Yuliana Cerri, Paulo Sergio Shokeen, Bhumika Lux, Renate Mima, Ewerton Garcia de Oliveira |
author_sort | Sakima, Vinicius Tatsuyuji |
collection | PubMed |
description | Denture stomatitis (DS) is a common infection in denture wearers, especially women. This study evaluated the induction of DS using acrylic devices attached to the palate of rats combined with inoculation of Candida spp. Immunocompetent male and female rats received a carbohydrate-rich diet. Impressions were taken from the rats’ palate to individually fabricate acrylic devices. Mono- and multispecies biofilms of C. albicans, C. glabrata, and C. tropicalis were grown on the devices, which were then cemented on posterior teeth and kept in the rats’ palate for four weeks. Microbial samples from the palate and the device were quantified. Oral microbiome of rats inoculated with C. albicans was analyzed by 16S rRNA gene sequencing. Log(10)(CFU/mL) were analyzed by mixed or two-way MANOVA (α = 0.05). Candida spp. and acrylic device did not induce palatal inflammation macroscopically nor microscopically. Although there was an increase (p < 0.001) of the total microbiota and female rats demonstrated higher (p = 0.007) recovery of Candida spp. from the palate, the gender differences were not biologically relevant. The microbiome results indicate an increase in inflammatory microbiota and reduction in health-associated micro-organisms. Although Candida spp. and acrylic device did not induce DS in immunocompetent rats, the shift in microbiota may precede manifestation of inflammation. |
format | Online Article Text |
id | pubmed-9147495 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91474952022-05-29 A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats Sakima, Vinicius Tatsuyuji Vega-Chacón, Yuliana Cerri, Paulo Sergio Shokeen, Bhumika Lux, Renate Mima, Ewerton Garcia de Oliveira J Fungi (Basel) Article Denture stomatitis (DS) is a common infection in denture wearers, especially women. This study evaluated the induction of DS using acrylic devices attached to the palate of rats combined with inoculation of Candida spp. Immunocompetent male and female rats received a carbohydrate-rich diet. Impressions were taken from the rats’ palate to individually fabricate acrylic devices. Mono- and multispecies biofilms of C. albicans, C. glabrata, and C. tropicalis were grown on the devices, which were then cemented on posterior teeth and kept in the rats’ palate for four weeks. Microbial samples from the palate and the device were quantified. Oral microbiome of rats inoculated with C. albicans was analyzed by 16S rRNA gene sequencing. Log(10)(CFU/mL) were analyzed by mixed or two-way MANOVA (α = 0.05). Candida spp. and acrylic device did not induce palatal inflammation macroscopically nor microscopically. Although there was an increase (p < 0.001) of the total microbiota and female rats demonstrated higher (p = 0.007) recovery of Candida spp. from the palate, the gender differences were not biologically relevant. The microbiome results indicate an increase in inflammatory microbiota and reduction in health-associated micro-organisms. Although Candida spp. and acrylic device did not induce DS in immunocompetent rats, the shift in microbiota may precede manifestation of inflammation. MDPI 2022-04-30 /pmc/articles/PMC9147495/ /pubmed/35628722 http://dx.doi.org/10.3390/jof8050466 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sakima, Vinicius Tatsuyuji Vega-Chacón, Yuliana Cerri, Paulo Sergio Shokeen, Bhumika Lux, Renate Mima, Ewerton Garcia de Oliveira A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats |
title | A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats |
title_full | A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats |
title_fullStr | A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats |
title_full_unstemmed | A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats |
title_short | A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats |
title_sort | denture use model associated with candida spp. in immunocompetent male and female rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147495/ https://www.ncbi.nlm.nih.gov/pubmed/35628722 http://dx.doi.org/10.3390/jof8050466 |
work_keys_str_mv | AT sakimaviniciustatsuyuji adentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats AT vegachaconyuliana adentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats AT cerripaulosergio adentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats AT shokeenbhumika adentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats AT luxrenate adentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats AT mimaewertongarciadeoliveira adentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats AT sakimaviniciustatsuyuji dentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats AT vegachaconyuliana dentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats AT cerripaulosergio dentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats AT shokeenbhumika dentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats AT luxrenate dentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats AT mimaewertongarciadeoliveira dentureusemodelassociatedwithcandidasppinimmunocompetentmaleandfemalerats |