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Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep

Blood loss in sheep can have different causes and may result in anemia. We aimed to evaluate the clinical, hematological, and biochemical alterations and the oxidative stress generated by acute blood loss. Eighteen healthy sheep underwent phlebotomy to remove 40% of the blood volume and were evaluat...

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Autores principales: Sousa, Rejane Santos, Sousa, Caroline Santos, Oliveira, Francisco Leonardo Costa, Firmino, Paulo Ricardo, Sousa, Isadora Karolina Freitas, Paula, Valeria Veras, Caruso, Nohora Mercado, Ortolani, Enrico Lippi, Minervino, Antonio Humberto Hamad, Barrêto-Júnior, Raimundo Alves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147502/
https://www.ncbi.nlm.nih.gov/pubmed/35622757
http://dx.doi.org/10.3390/vetsci9050229
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author Sousa, Rejane Santos
Sousa, Caroline Santos
Oliveira, Francisco Leonardo Costa
Firmino, Paulo Ricardo
Sousa, Isadora Karolina Freitas
Paula, Valeria Veras
Caruso, Nohora Mercado
Ortolani, Enrico Lippi
Minervino, Antonio Humberto Hamad
Barrêto-Júnior, Raimundo Alves
author_facet Sousa, Rejane Santos
Sousa, Caroline Santos
Oliveira, Francisco Leonardo Costa
Firmino, Paulo Ricardo
Sousa, Isadora Karolina Freitas
Paula, Valeria Veras
Caruso, Nohora Mercado
Ortolani, Enrico Lippi
Minervino, Antonio Humberto Hamad
Barrêto-Júnior, Raimundo Alves
author_sort Sousa, Rejane Santos
collection PubMed
description Blood loss in sheep can have different causes and may result in anemia. We aimed to evaluate the clinical, hematological, and biochemical alterations and the oxidative stress generated by acute blood loss. Eighteen healthy sheep underwent phlebotomy to remove 40% of the blood volume and were evaluated clinically and by laboratory tests for clinical, biochemical, and blood gas variables and to assess oxidative stress before induction (T0), 30 min (T30 min), and 6 (T6 h), 12 (T12 h), and 24 h (T24 h) after blood loss. The sheep showed tachycardia from T30 min until T24 h, reduction in the hematocrit, number of erythrocytes, and hemoglobin concentration, with lower values at T24 h and increase in the number of leukocytes from T12 h on. There was a reduction in blood pH and oxygen pressure at T30 min, increased lactate concentration and reduced blood bicarbonate at this time. There was an increase in urea concentration from T6 h until the end of the study, with no change in creatinine levels. The animals did not show changes in the concentration of malonaldehyde, and in the activity of the enzymes superoxide dismutase, glutathione peroxidase, and catalase, but there was a reduction in the concentration of reduced glutathione at T24 h. The acute loss of 40% of blood volume is capable of promoting relevant clinical, hematological, blood gas, and biochemical alterations, and contributed to the appearance of oxidative stress with reduced glutathione concentration, suggesting that this process generated free radicals in sufficient quantity to diminish the action of antioxidants.
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spelling pubmed-91475022022-05-29 Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep Sousa, Rejane Santos Sousa, Caroline Santos Oliveira, Francisco Leonardo Costa Firmino, Paulo Ricardo Sousa, Isadora Karolina Freitas Paula, Valeria Veras Caruso, Nohora Mercado Ortolani, Enrico Lippi Minervino, Antonio Humberto Hamad Barrêto-Júnior, Raimundo Alves Vet Sci Article Blood loss in sheep can have different causes and may result in anemia. We aimed to evaluate the clinical, hematological, and biochemical alterations and the oxidative stress generated by acute blood loss. Eighteen healthy sheep underwent phlebotomy to remove 40% of the blood volume and were evaluated clinically and by laboratory tests for clinical, biochemical, and blood gas variables and to assess oxidative stress before induction (T0), 30 min (T30 min), and 6 (T6 h), 12 (T12 h), and 24 h (T24 h) after blood loss. The sheep showed tachycardia from T30 min until T24 h, reduction in the hematocrit, number of erythrocytes, and hemoglobin concentration, with lower values at T24 h and increase in the number of leukocytes from T12 h on. There was a reduction in blood pH and oxygen pressure at T30 min, increased lactate concentration and reduced blood bicarbonate at this time. There was an increase in urea concentration from T6 h until the end of the study, with no change in creatinine levels. The animals did not show changes in the concentration of malonaldehyde, and in the activity of the enzymes superoxide dismutase, glutathione peroxidase, and catalase, but there was a reduction in the concentration of reduced glutathione at T24 h. The acute loss of 40% of blood volume is capable of promoting relevant clinical, hematological, blood gas, and biochemical alterations, and contributed to the appearance of oxidative stress with reduced glutathione concentration, suggesting that this process generated free radicals in sufficient quantity to diminish the action of antioxidants. MDPI 2022-05-11 /pmc/articles/PMC9147502/ /pubmed/35622757 http://dx.doi.org/10.3390/vetsci9050229 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sousa, Rejane Santos
Sousa, Caroline Santos
Oliveira, Francisco Leonardo Costa
Firmino, Paulo Ricardo
Sousa, Isadora Karolina Freitas
Paula, Valeria Veras
Caruso, Nohora Mercado
Ortolani, Enrico Lippi
Minervino, Antonio Humberto Hamad
Barrêto-Júnior, Raimundo Alves
Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep
title Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep
title_full Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep
title_fullStr Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep
title_full_unstemmed Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep
title_short Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep
title_sort impact of acute blood loss on clinical, hematological, biochemical, and oxidative stress variables in sheep
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147502/
https://www.ncbi.nlm.nih.gov/pubmed/35622757
http://dx.doi.org/10.3390/vetsci9050229
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