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Physiological Importance of Molybdate Transporter Family 1 in Feeding the Molybdenum Cofactor Biosynthesis Pathway in Arabidopsis thaliana

Molybdate uptake and molybdenum cofactor (Moco) biosynthesis were investigated in detail in the last few decades. The present study critically reviews our present knowledge about eukaryotic molybdate transporters (MOT) and focuses on the model plant Arabidopsis thaliana, complementing it with new ex...

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Autores principales: Minner-Meinen, Rieke, Weber, Jan-Niklas, Kistner, Sarah, Meyfarth, Paul, Saudhof, Merve, van den Hout, Lena, Schulze, Jutta, Mendel, Ralf-Rainer, Hänsch, Robert, Kaufholdt, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147641/
https://www.ncbi.nlm.nih.gov/pubmed/35630635
http://dx.doi.org/10.3390/molecules27103158
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author Minner-Meinen, Rieke
Weber, Jan-Niklas
Kistner, Sarah
Meyfarth, Paul
Saudhof, Merve
van den Hout, Lena
Schulze, Jutta
Mendel, Ralf-Rainer
Hänsch, Robert
Kaufholdt, David
author_facet Minner-Meinen, Rieke
Weber, Jan-Niklas
Kistner, Sarah
Meyfarth, Paul
Saudhof, Merve
van den Hout, Lena
Schulze, Jutta
Mendel, Ralf-Rainer
Hänsch, Robert
Kaufholdt, David
author_sort Minner-Meinen, Rieke
collection PubMed
description Molybdate uptake and molybdenum cofactor (Moco) biosynthesis were investigated in detail in the last few decades. The present study critically reviews our present knowledge about eukaryotic molybdate transporters (MOT) and focuses on the model plant Arabidopsis thaliana, complementing it with new experiments, filling missing gaps, and clarifying contradictory results in the literature. Two molybdate transporters, MOT1.1 and MOT1.2, are known in Arabidopsis, but their importance for sufficient molybdate supply to Moco biosynthesis remains unclear. For a better understanding of their physiological functions in molybdate homeostasis, we studied the impact of mot1.1 and mot1.2 knock-out mutants, including a double knock-out on molybdate uptake and Moco-dependent enzyme activity, MOT localisation, and protein–protein interactions. The outcome illustrates different physiological roles for Moco biosynthesis: MOT1.1 is plasma membrane located and its function lies in the efficient absorption of molybdate from soil and its distribution throughout the plant. However, MOT1.1 is not involved in leaf cell imports of molybdate and has no interaction with proteins of the Moco biosynthesis complex. In contrast, the tonoplast-localised transporter MOT1.2 exports molybdate stored in the vacuole and makes it available for re-localisation during senescence. It also supplies the Moco biosynthesis complex with molybdate by direct interaction with molybdenum insertase Cnx1 for controlled and safe sequestering.
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spelling pubmed-91476412022-05-29 Physiological Importance of Molybdate Transporter Family 1 in Feeding the Molybdenum Cofactor Biosynthesis Pathway in Arabidopsis thaliana Minner-Meinen, Rieke Weber, Jan-Niklas Kistner, Sarah Meyfarth, Paul Saudhof, Merve van den Hout, Lena Schulze, Jutta Mendel, Ralf-Rainer Hänsch, Robert Kaufholdt, David Molecules Article Molybdate uptake and molybdenum cofactor (Moco) biosynthesis were investigated in detail in the last few decades. The present study critically reviews our present knowledge about eukaryotic molybdate transporters (MOT) and focuses on the model plant Arabidopsis thaliana, complementing it with new experiments, filling missing gaps, and clarifying contradictory results in the literature. Two molybdate transporters, MOT1.1 and MOT1.2, are known in Arabidopsis, but their importance for sufficient molybdate supply to Moco biosynthesis remains unclear. For a better understanding of their physiological functions in molybdate homeostasis, we studied the impact of mot1.1 and mot1.2 knock-out mutants, including a double knock-out on molybdate uptake and Moco-dependent enzyme activity, MOT localisation, and protein–protein interactions. The outcome illustrates different physiological roles for Moco biosynthesis: MOT1.1 is plasma membrane located and its function lies in the efficient absorption of molybdate from soil and its distribution throughout the plant. However, MOT1.1 is not involved in leaf cell imports of molybdate and has no interaction with proteins of the Moco biosynthesis complex. In contrast, the tonoplast-localised transporter MOT1.2 exports molybdate stored in the vacuole and makes it available for re-localisation during senescence. It also supplies the Moco biosynthesis complex with molybdate by direct interaction with molybdenum insertase Cnx1 for controlled and safe sequestering. MDPI 2022-05-15 /pmc/articles/PMC9147641/ /pubmed/35630635 http://dx.doi.org/10.3390/molecules27103158 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Minner-Meinen, Rieke
Weber, Jan-Niklas
Kistner, Sarah
Meyfarth, Paul
Saudhof, Merve
van den Hout, Lena
Schulze, Jutta
Mendel, Ralf-Rainer
Hänsch, Robert
Kaufholdt, David
Physiological Importance of Molybdate Transporter Family 1 in Feeding the Molybdenum Cofactor Biosynthesis Pathway in Arabidopsis thaliana
title Physiological Importance of Molybdate Transporter Family 1 in Feeding the Molybdenum Cofactor Biosynthesis Pathway in Arabidopsis thaliana
title_full Physiological Importance of Molybdate Transporter Family 1 in Feeding the Molybdenum Cofactor Biosynthesis Pathway in Arabidopsis thaliana
title_fullStr Physiological Importance of Molybdate Transporter Family 1 in Feeding the Molybdenum Cofactor Biosynthesis Pathway in Arabidopsis thaliana
title_full_unstemmed Physiological Importance of Molybdate Transporter Family 1 in Feeding the Molybdenum Cofactor Biosynthesis Pathway in Arabidopsis thaliana
title_short Physiological Importance of Molybdate Transporter Family 1 in Feeding the Molybdenum Cofactor Biosynthesis Pathway in Arabidopsis thaliana
title_sort physiological importance of molybdate transporter family 1 in feeding the molybdenum cofactor biosynthesis pathway in arabidopsis thaliana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147641/
https://www.ncbi.nlm.nih.gov/pubmed/35630635
http://dx.doi.org/10.3390/molecules27103158
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