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Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus

RNA viruses, such as foot-and-mouth disease virus (FMDV), have error-prone replication resulting in the continuous emergence of new viral strains capable of evading current vaccine coverage. Vaccine formulations must be regularly updated, which is both costly and technically challenging for many vac...

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Autores principales: Puckette, Michael, Primavera, Victoria, Martel, Erica, Barrera, Jose, Hurtle, William, Clark, Benjamin, Kamicker, Barbara, Zurita, Mariceny, Brake, David, Neilan, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147724/
https://www.ncbi.nlm.nih.gov/pubmed/35632734
http://dx.doi.org/10.3390/v14050989
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author Puckette, Michael
Primavera, Victoria
Martel, Erica
Barrera, Jose
Hurtle, William
Clark, Benjamin
Kamicker, Barbara
Zurita, Mariceny
Brake, David
Neilan, John
author_facet Puckette, Michael
Primavera, Victoria
Martel, Erica
Barrera, Jose
Hurtle, William
Clark, Benjamin
Kamicker, Barbara
Zurita, Mariceny
Brake, David
Neilan, John
author_sort Puckette, Michael
collection PubMed
description RNA viruses, such as foot-and-mouth disease virus (FMDV), have error-prone replication resulting in the continuous emergence of new viral strains capable of evading current vaccine coverage. Vaccine formulations must be regularly updated, which is both costly and technically challenging for many vaccine platforms. In this report, we describe a plasmid-based virus-like particle (VLP) production platform utilizing transiently transfected mammalian cell cultures that combines both the rapid response adaptability of nucleic-acid-based vaccines with the ability to produce intact capsid epitopes required for immunity. Formulated vaccines which employed this platform conferred complete protection from clinical foot-and-mouth disease in both swine and cattle. This novel platform can be quickly adapted to new viral strains and serotypes through targeted exchanges of only the FMDV capsid polypeptide nucleic acid sequences, from which processed structural capsid proteins are derived. This platform obviates the need for high biocontainment manufacturing facilities to produce inactivated whole-virus vaccines from infected mammalian cell cultures, which requires upstream expansion and downstream concentration of large quantities of live virulent viruses.
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spelling pubmed-91477242022-05-29 Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus Puckette, Michael Primavera, Victoria Martel, Erica Barrera, Jose Hurtle, William Clark, Benjamin Kamicker, Barbara Zurita, Mariceny Brake, David Neilan, John Viruses Article RNA viruses, such as foot-and-mouth disease virus (FMDV), have error-prone replication resulting in the continuous emergence of new viral strains capable of evading current vaccine coverage. Vaccine formulations must be regularly updated, which is both costly and technically challenging for many vaccine platforms. In this report, we describe a plasmid-based virus-like particle (VLP) production platform utilizing transiently transfected mammalian cell cultures that combines both the rapid response adaptability of nucleic-acid-based vaccines with the ability to produce intact capsid epitopes required for immunity. Formulated vaccines which employed this platform conferred complete protection from clinical foot-and-mouth disease in both swine and cattle. This novel platform can be quickly adapted to new viral strains and serotypes through targeted exchanges of only the FMDV capsid polypeptide nucleic acid sequences, from which processed structural capsid proteins are derived. This platform obviates the need for high biocontainment manufacturing facilities to produce inactivated whole-virus vaccines from infected mammalian cell cultures, which requires upstream expansion and downstream concentration of large quantities of live virulent viruses. MDPI 2022-05-07 /pmc/articles/PMC9147724/ /pubmed/35632734 http://dx.doi.org/10.3390/v14050989 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Puckette, Michael
Primavera, Victoria
Martel, Erica
Barrera, Jose
Hurtle, William
Clark, Benjamin
Kamicker, Barbara
Zurita, Mariceny
Brake, David
Neilan, John
Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus
title Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus
title_full Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus
title_fullStr Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus
title_full_unstemmed Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus
title_short Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus
title_sort transiently transfected mammalian cell cultures: an adaptable and effective platform for virus-like particle-based vaccines against foot-and-mouth disease virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147724/
https://www.ncbi.nlm.nih.gov/pubmed/35632734
http://dx.doi.org/10.3390/v14050989
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