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The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1

Pathogen associated molecular pattern (PAMP) triggered immunity (PTI) is the first line of plant defense. We hypothesized that the absence of pattern recognition receptors (PRRs) in plants could influence the rhizosphere microbiome. Here, we report sequencing of the 16S ribosomal RNA gene and the fu...

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Autores principales: Fonseca, Jose P., Lakshmanan, Venkatachalam, Boschiero, Clarissa, Mysore, Kirankumar S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147754/
https://www.ncbi.nlm.nih.gov/pubmed/35631748
http://dx.doi.org/10.3390/plants11101323
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author Fonseca, Jose P.
Lakshmanan, Venkatachalam
Boschiero, Clarissa
Mysore, Kirankumar S.
author_facet Fonseca, Jose P.
Lakshmanan, Venkatachalam
Boschiero, Clarissa
Mysore, Kirankumar S.
author_sort Fonseca, Jose P.
collection PubMed
description Pathogen associated molecular pattern (PAMP) triggered immunity (PTI) is the first line of plant defense. We hypothesized that the absence of pattern recognition receptors (PRRs) in plants could influence the rhizosphere microbiome. Here, we report sequencing of the 16S ribosomal RNA gene and the fungal ribosomal RNA internal transcribed spacer region of rhizosphere DNA from three Arabidopsis PRR mutants involved in plant innate immunity (efr1, fls2, and cerk1). We conducted experiments in a growth chamber using native soil from the Red River Farm (Terral, OK, USA) to detect microbial community shifts in the rhizosphere that may occur in the absence of PRR receptors compared to wild-type (WT; Col-0) plants. No difference in the α-diversity of the rhizosphere microbial population was observed between the PRR mutants tested and the WT. Plant host genotype had a significant impact in bacterial β-diversity only between the fls2 mutant and the WT. Surprisingly, no significant changes in fungal β-diversity were observed between the PRR mutants and WT, although we observed an increase in relative abundance for the cup fungi (Pezizaceae) in the cerk1 mutant. This finding suggests that the FLS2 receptor can modulate the rhizosphere-associated microbiome β-diversity and expands the list of current known genotypes that can modulate the rhizosphere microbiota.
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spelling pubmed-91477542022-05-29 The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1 Fonseca, Jose P. Lakshmanan, Venkatachalam Boschiero, Clarissa Mysore, Kirankumar S. Plants (Basel) Communication Pathogen associated molecular pattern (PAMP) triggered immunity (PTI) is the first line of plant defense. We hypothesized that the absence of pattern recognition receptors (PRRs) in plants could influence the rhizosphere microbiome. Here, we report sequencing of the 16S ribosomal RNA gene and the fungal ribosomal RNA internal transcribed spacer region of rhizosphere DNA from three Arabidopsis PRR mutants involved in plant innate immunity (efr1, fls2, and cerk1). We conducted experiments in a growth chamber using native soil from the Red River Farm (Terral, OK, USA) to detect microbial community shifts in the rhizosphere that may occur in the absence of PRR receptors compared to wild-type (WT; Col-0) plants. No difference in the α-diversity of the rhizosphere microbial population was observed between the PRR mutants tested and the WT. Plant host genotype had a significant impact in bacterial β-diversity only between the fls2 mutant and the WT. Surprisingly, no significant changes in fungal β-diversity were observed between the PRR mutants and WT, although we observed an increase in relative abundance for the cup fungi (Pezizaceae) in the cerk1 mutant. This finding suggests that the FLS2 receptor can modulate the rhizosphere-associated microbiome β-diversity and expands the list of current known genotypes that can modulate the rhizosphere microbiota. MDPI 2022-05-17 /pmc/articles/PMC9147754/ /pubmed/35631748 http://dx.doi.org/10.3390/plants11101323 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Fonseca, Jose P.
Lakshmanan, Venkatachalam
Boschiero, Clarissa
Mysore, Kirankumar S.
The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1
title The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1
title_full The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1
title_fullStr The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1
title_full_unstemmed The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1
title_short The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1
title_sort pattern recognition receptor fls2 can shape the arabidopsis rhizosphere microbiome β-diversity but not efr1 and cerk1
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147754/
https://www.ncbi.nlm.nih.gov/pubmed/35631748
http://dx.doi.org/10.3390/plants11101323
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