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Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis

The aim of the current study is to present a low-cost and easy-to-interpret colorimetric kit used to diagnose porcine circovirus 2 (PCV-2) to the naked eye, without any specific equipment. The aforementioned kit used as base hybrid nanoparticles resulting from the merge of surface active maghemite n...

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Autores principales: Basso, Caroline Rodrigues, Yamakawa, Ana Carolina, Cruz, Taís Fukuta, Pedrosa, Valber Albuquerque, Magro, Massimiliano, Vianello, Fabio, Araújo Júnior, João Pessoa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147935/
https://www.ncbi.nlm.nih.gov/pubmed/35631091
http://dx.doi.org/10.3390/pathogens11050570
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author Basso, Caroline Rodrigues
Yamakawa, Ana Carolina
Cruz, Taís Fukuta
Pedrosa, Valber Albuquerque
Magro, Massimiliano
Vianello, Fabio
Araújo Júnior, João Pessoa
author_facet Basso, Caroline Rodrigues
Yamakawa, Ana Carolina
Cruz, Taís Fukuta
Pedrosa, Valber Albuquerque
Magro, Massimiliano
Vianello, Fabio
Araújo Júnior, João Pessoa
author_sort Basso, Caroline Rodrigues
collection PubMed
description The aim of the current study is to present a low-cost and easy-to-interpret colorimetric kit used to diagnose porcine circovirus 2 (PCV-2) to the naked eye, without any specific equipment. The aforementioned kit used as base hybrid nanoparticles resulting from the merge of surface active maghemite nanoparticles and gold nanoparticles, based on the deposition of specific PCV-2 antibodies on their surface through covalent bonds. In total, 10 negative and 40 positive samples (≥10(2) DNA copies/µL of serum) confirmed by qPCR technique were tested. PCV-1 virus, adenovirus, and parvovirus samples were tested as interferents to rule out likely false-positive results. Positive samples showed purple color when they were added to the complex, whereas negative samples showed red color; they were visible to the naked eye. The entire color-change process took place approximately 1 min after the analyzed samples were added to the complex. They were tested at different dilutions, namely pure, 1:10, 1:100, 1:1000, and 1:10,000. Localized surface plasmon resonance (LSPR) and transmission electron microscopy (TEM) images were generated to validate the experiment. This new real-time PCV-2 diagnostic methodology emerged as simple and economic alternative to traditional tests since the final price of the kit is USD 4.00.
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spelling pubmed-91479352022-05-29 Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis Basso, Caroline Rodrigues Yamakawa, Ana Carolina Cruz, Taís Fukuta Pedrosa, Valber Albuquerque Magro, Massimiliano Vianello, Fabio Araújo Júnior, João Pessoa Pathogens Article The aim of the current study is to present a low-cost and easy-to-interpret colorimetric kit used to diagnose porcine circovirus 2 (PCV-2) to the naked eye, without any specific equipment. The aforementioned kit used as base hybrid nanoparticles resulting from the merge of surface active maghemite nanoparticles and gold nanoparticles, based on the deposition of specific PCV-2 antibodies on their surface through covalent bonds. In total, 10 negative and 40 positive samples (≥10(2) DNA copies/µL of serum) confirmed by qPCR technique were tested. PCV-1 virus, adenovirus, and parvovirus samples were tested as interferents to rule out likely false-positive results. Positive samples showed purple color when they were added to the complex, whereas negative samples showed red color; they were visible to the naked eye. The entire color-change process took place approximately 1 min after the analyzed samples were added to the complex. They were tested at different dilutions, namely pure, 1:10, 1:100, 1:1000, and 1:10,000. Localized surface plasmon resonance (LSPR) and transmission electron microscopy (TEM) images were generated to validate the experiment. This new real-time PCV-2 diagnostic methodology emerged as simple and economic alternative to traditional tests since the final price of the kit is USD 4.00. MDPI 2022-05-12 /pmc/articles/PMC9147935/ /pubmed/35631091 http://dx.doi.org/10.3390/pathogens11050570 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Basso, Caroline Rodrigues
Yamakawa, Ana Carolina
Cruz, Taís Fukuta
Pedrosa, Valber Albuquerque
Magro, Massimiliano
Vianello, Fabio
Araújo Júnior, João Pessoa
Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis
title Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis
title_full Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis
title_fullStr Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis
title_full_unstemmed Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis
title_short Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis
title_sort colorimetric kit for rapid porcine circovirus 2 (pcv-2) diagnosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147935/
https://www.ncbi.nlm.nih.gov/pubmed/35631091
http://dx.doi.org/10.3390/pathogens11050570
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