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Liver Graft Pathology and Low Serum 25-Hydroxyvitamin D after Living Donor Liver Transplantation
Background: Most cases of advanced liver diseases are associated with low serum 25-hydroxyvitamin D and vitamin D deficiency. This phenomenon may occur in living donor liver transplantation (LDLT). Aims: We conducted this study to explore the interplay between VDR and CYP2R1 in liver graft and compa...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147938/ https://www.ncbi.nlm.nih.gov/pubmed/35629892 http://dx.doi.org/10.3390/metabo12050388 |
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author | Lin, Shu-Hsien Wang, Chih-Chi Huang, Kuang-Tzu Chen, Kuang-Den Hsu, Li-Wen Eng, Hock-Liew Chiu, King-Wah |
author_facet | Lin, Shu-Hsien Wang, Chih-Chi Huang, Kuang-Tzu Chen, Kuang-Den Hsu, Li-Wen Eng, Hock-Liew Chiu, King-Wah |
author_sort | Lin, Shu-Hsien |
collection | PubMed |
description | Background: Most cases of advanced liver diseases are associated with low serum 25-hydroxyvitamin D and vitamin D deficiency. This phenomenon may occur in living donor liver transplantation (LDLT). Aims: We conducted this study to explore the interplay between VDR and CYP2R1 in liver graft and compared our findings with the pathological interpretation of serum 25(OH)D concentration. Methods: In total, 60 patients received liver graft biopsy after LDLT and were separated (1:1) into two groups: graft rejection group and graft non-rejection group. We extracted both of the recipients’ and donors’ serum DNA to investigate the vitamin D receptor (VDR) rs2228530 and CYP2R1 rs10741657 single nucleotide polymorphisms (SNPs) using real-time polymerase chain reaction. We also extracted DNA from liver graft tissues to explore the genetic alleles of VDR rs2228530 and CYP2R1 rs10741657 after LDLT. Serum biochemistry profile and 25(OH)D concentrations were measured before and after LDLT. Results: There were no significant differences in serum VDR rs2228530 and CYP2R1 rs10741657 genetic alleles between recipients and donors. The percentage of genetic modification was 33.4% (10/30) for the rejection and non-rejection groups in VDR rs2228530, and 66.7% (20/30) for both groups in CYP2R1 rs10741657. Serum 25(OH)D concentrations were significantly lower after LDLT D30 than that before LDLT in the rejection (p = 0.0001) and non-rejection graft pathology (p = 0.0017) groups. Conclusions: The presence of low serum 25(OH)D concentrations after LDLT suggested that post-transplant low serum 25(OH)D concentrations may develop with the homogenous phenomenon of VDR rs2228530 and CYP2R1 rs10741657 genetic modifications in recipients regardless of graft pathology. |
format | Online Article Text |
id | pubmed-9147938 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91479382022-05-29 Liver Graft Pathology and Low Serum 25-Hydroxyvitamin D after Living Donor Liver Transplantation Lin, Shu-Hsien Wang, Chih-Chi Huang, Kuang-Tzu Chen, Kuang-Den Hsu, Li-Wen Eng, Hock-Liew Chiu, King-Wah Metabolites Article Background: Most cases of advanced liver diseases are associated with low serum 25-hydroxyvitamin D and vitamin D deficiency. This phenomenon may occur in living donor liver transplantation (LDLT). Aims: We conducted this study to explore the interplay between VDR and CYP2R1 in liver graft and compared our findings with the pathological interpretation of serum 25(OH)D concentration. Methods: In total, 60 patients received liver graft biopsy after LDLT and were separated (1:1) into two groups: graft rejection group and graft non-rejection group. We extracted both of the recipients’ and donors’ serum DNA to investigate the vitamin D receptor (VDR) rs2228530 and CYP2R1 rs10741657 single nucleotide polymorphisms (SNPs) using real-time polymerase chain reaction. We also extracted DNA from liver graft tissues to explore the genetic alleles of VDR rs2228530 and CYP2R1 rs10741657 after LDLT. Serum biochemistry profile and 25(OH)D concentrations were measured before and after LDLT. Results: There were no significant differences in serum VDR rs2228530 and CYP2R1 rs10741657 genetic alleles between recipients and donors. The percentage of genetic modification was 33.4% (10/30) for the rejection and non-rejection groups in VDR rs2228530, and 66.7% (20/30) for both groups in CYP2R1 rs10741657. Serum 25(OH)D concentrations were significantly lower after LDLT D30 than that before LDLT in the rejection (p = 0.0001) and non-rejection graft pathology (p = 0.0017) groups. Conclusions: The presence of low serum 25(OH)D concentrations after LDLT suggested that post-transplant low serum 25(OH)D concentrations may develop with the homogenous phenomenon of VDR rs2228530 and CYP2R1 rs10741657 genetic modifications in recipients regardless of graft pathology. MDPI 2022-04-25 /pmc/articles/PMC9147938/ /pubmed/35629892 http://dx.doi.org/10.3390/metabo12050388 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lin, Shu-Hsien Wang, Chih-Chi Huang, Kuang-Tzu Chen, Kuang-Den Hsu, Li-Wen Eng, Hock-Liew Chiu, King-Wah Liver Graft Pathology and Low Serum 25-Hydroxyvitamin D after Living Donor Liver Transplantation |
title | Liver Graft Pathology and Low Serum 25-Hydroxyvitamin D after Living Donor Liver Transplantation |
title_full | Liver Graft Pathology and Low Serum 25-Hydroxyvitamin D after Living Donor Liver Transplantation |
title_fullStr | Liver Graft Pathology and Low Serum 25-Hydroxyvitamin D after Living Donor Liver Transplantation |
title_full_unstemmed | Liver Graft Pathology and Low Serum 25-Hydroxyvitamin D after Living Donor Liver Transplantation |
title_short | Liver Graft Pathology and Low Serum 25-Hydroxyvitamin D after Living Donor Liver Transplantation |
title_sort | liver graft pathology and low serum 25-hydroxyvitamin d after living donor liver transplantation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147938/ https://www.ncbi.nlm.nih.gov/pubmed/35629892 http://dx.doi.org/10.3390/metabo12050388 |
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