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A Genome-Wide Association Study Identifying Single-Nucleotide Polymorphisms for Iron and Zinc Biofortification in a Worldwide Barley Collection

Micronutrient deficiency affects half of the world’s population, mostly in developing countries. Severe health issues such as anemia and inadequate growth in children below five years of age and pregnant women have been linked to mineral deficiencies (mostly zinc and iron). Improving the mineral con...

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Autores principales: Nyiraguhirwa, Solange, Grana, Zahra, Ouabbou, Hassan, Iraqi, Driss, Ibriz, Mohammed, Mamidi, Sujan, Udupa, Sripada M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148054/
https://www.ncbi.nlm.nih.gov/pubmed/35631775
http://dx.doi.org/10.3390/plants11101349
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author Nyiraguhirwa, Solange
Grana, Zahra
Ouabbou, Hassan
Iraqi, Driss
Ibriz, Mohammed
Mamidi, Sujan
Udupa, Sripada M.
author_facet Nyiraguhirwa, Solange
Grana, Zahra
Ouabbou, Hassan
Iraqi, Driss
Ibriz, Mohammed
Mamidi, Sujan
Udupa, Sripada M.
author_sort Nyiraguhirwa, Solange
collection PubMed
description Micronutrient deficiency affects half of the world’s population, mostly in developing countries. Severe health issues such as anemia and inadequate growth in children below five years of age and pregnant women have been linked to mineral deficiencies (mostly zinc and iron). Improving the mineral content in staple crops, also known as mineral biofortification, remains the best approach to address mineral malnutrition. Barley is a staple crop in some parts of the world and is a healthy choice since it contains β-glucan, a high dietary protein. Barley mineral biofortification, especially with zinc and iron, can be beneficial since barley easily adapts to marginalized areas and requires less input than other frequently consumed cereals. In this study, we analyzed zinc and iron content in 496 barley samples. The samples were genotyped with an Illumina 50 K SNP chip. Genome-wide association studies (GWAS) identified 62 SNPs and 68 SNPs (p < 0.001) associated with iron and zinc content in grains, respectively. After a Bonferroni correction (p < 0.005), there were 12 SNPs (single-nucleotide polymorphism) associated with Zn and 6 for iron. SNP annotations revealed proteins involved in membrane transport, Zn and Fe binding, linked to nutrient remobilization in grains. These results can be used to develop biofortified barley via marker-assisted selection (MAS), which could alleviate mineral malnutrition.
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spelling pubmed-91480542022-05-29 A Genome-Wide Association Study Identifying Single-Nucleotide Polymorphisms for Iron and Zinc Biofortification in a Worldwide Barley Collection Nyiraguhirwa, Solange Grana, Zahra Ouabbou, Hassan Iraqi, Driss Ibriz, Mohammed Mamidi, Sujan Udupa, Sripada M. Plants (Basel) Article Micronutrient deficiency affects half of the world’s population, mostly in developing countries. Severe health issues such as anemia and inadequate growth in children below five years of age and pregnant women have been linked to mineral deficiencies (mostly zinc and iron). Improving the mineral content in staple crops, also known as mineral biofortification, remains the best approach to address mineral malnutrition. Barley is a staple crop in some parts of the world and is a healthy choice since it contains β-glucan, a high dietary protein. Barley mineral biofortification, especially with zinc and iron, can be beneficial since barley easily adapts to marginalized areas and requires less input than other frequently consumed cereals. In this study, we analyzed zinc and iron content in 496 barley samples. The samples were genotyped with an Illumina 50 K SNP chip. Genome-wide association studies (GWAS) identified 62 SNPs and 68 SNPs (p < 0.001) associated with iron and zinc content in grains, respectively. After a Bonferroni correction (p < 0.005), there were 12 SNPs (single-nucleotide polymorphism) associated with Zn and 6 for iron. SNP annotations revealed proteins involved in membrane transport, Zn and Fe binding, linked to nutrient remobilization in grains. These results can be used to develop biofortified barley via marker-assisted selection (MAS), which could alleviate mineral malnutrition. MDPI 2022-05-19 /pmc/articles/PMC9148054/ /pubmed/35631775 http://dx.doi.org/10.3390/plants11101349 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nyiraguhirwa, Solange
Grana, Zahra
Ouabbou, Hassan
Iraqi, Driss
Ibriz, Mohammed
Mamidi, Sujan
Udupa, Sripada M.
A Genome-Wide Association Study Identifying Single-Nucleotide Polymorphisms for Iron and Zinc Biofortification in a Worldwide Barley Collection
title A Genome-Wide Association Study Identifying Single-Nucleotide Polymorphisms for Iron and Zinc Biofortification in a Worldwide Barley Collection
title_full A Genome-Wide Association Study Identifying Single-Nucleotide Polymorphisms for Iron and Zinc Biofortification in a Worldwide Barley Collection
title_fullStr A Genome-Wide Association Study Identifying Single-Nucleotide Polymorphisms for Iron and Zinc Biofortification in a Worldwide Barley Collection
title_full_unstemmed A Genome-Wide Association Study Identifying Single-Nucleotide Polymorphisms for Iron and Zinc Biofortification in a Worldwide Barley Collection
title_short A Genome-Wide Association Study Identifying Single-Nucleotide Polymorphisms for Iron and Zinc Biofortification in a Worldwide Barley Collection
title_sort genome-wide association study identifying single-nucleotide polymorphisms for iron and zinc biofortification in a worldwide barley collection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148054/
https://www.ncbi.nlm.nih.gov/pubmed/35631775
http://dx.doi.org/10.3390/plants11101349
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