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The First Report on Transgenic Hairy Root Induction from the Stem of Tung Tree (Vernicia fordii)

Tung tree (Vernicia fordii) is an industrially important oil-bearing woody plant of the Euphorbiaceae family. Functional studies of tung tree at the molecular level are limited by the lack of an efficient transgenic system. The Agrobacterium rhizogenes-mediated hairy root generation system is an alt...

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Detalles Bibliográficos
Autores principales: Jia, Hongyu, Chen, Junjie, Zhang, Lin, Zhang, Lingling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148109/
https://www.ncbi.nlm.nih.gov/pubmed/35631740
http://dx.doi.org/10.3390/plants11101315
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author Jia, Hongyu
Chen, Junjie
Zhang, Lin
Zhang, Lingling
author_facet Jia, Hongyu
Chen, Junjie
Zhang, Lin
Zhang, Lingling
author_sort Jia, Hongyu
collection PubMed
description Tung tree (Vernicia fordii) is an industrially important oil-bearing woody plant of the Euphorbiaceae family. Functional studies of tung tree at the molecular level are limited by the lack of an efficient transgenic system. The Agrobacterium rhizogenes-mediated hairy root generation system is an alternative to typical plant transformation systems. However, its application in many plants has been blocked due to the inability of existing methods to induce hairy roots. Thus, it is critical to build a method suitable for the hairy induction of the specific plant of interest. In this study, a modified method for tung tree was developed, and it is the first report that hairy roots could be effectively induced in the stem of tung tree. With the method, an average of 10.7 hairy roots per seedling were generated in tung tree, approximately 67% of seedlings produced transgenic hairy roots and approximately 13.96% of the hairy roots of these seedlings were transgenic. This modified method is also suitable for the hairy root induction of two other oil-bearing plants of the Euphorbiaceae family, Ricinus communis and Vernicia montana. This modified method will accelerate functional studies of tung tree at the molecular level and also shed light on plants lacking a transgenic system.
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spelling pubmed-91481092022-05-29 The First Report on Transgenic Hairy Root Induction from the Stem of Tung Tree (Vernicia fordii) Jia, Hongyu Chen, Junjie Zhang, Lin Zhang, Lingling Plants (Basel) Article Tung tree (Vernicia fordii) is an industrially important oil-bearing woody plant of the Euphorbiaceae family. Functional studies of tung tree at the molecular level are limited by the lack of an efficient transgenic system. The Agrobacterium rhizogenes-mediated hairy root generation system is an alternative to typical plant transformation systems. However, its application in many plants has been blocked due to the inability of existing methods to induce hairy roots. Thus, it is critical to build a method suitable for the hairy induction of the specific plant of interest. In this study, a modified method for tung tree was developed, and it is the first report that hairy roots could be effectively induced in the stem of tung tree. With the method, an average of 10.7 hairy roots per seedling were generated in tung tree, approximately 67% of seedlings produced transgenic hairy roots and approximately 13.96% of the hairy roots of these seedlings were transgenic. This modified method is also suitable for the hairy root induction of two other oil-bearing plants of the Euphorbiaceae family, Ricinus communis and Vernicia montana. This modified method will accelerate functional studies of tung tree at the molecular level and also shed light on plants lacking a transgenic system. MDPI 2022-05-16 /pmc/articles/PMC9148109/ /pubmed/35631740 http://dx.doi.org/10.3390/plants11101315 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jia, Hongyu
Chen, Junjie
Zhang, Lin
Zhang, Lingling
The First Report on Transgenic Hairy Root Induction from the Stem of Tung Tree (Vernicia fordii)
title The First Report on Transgenic Hairy Root Induction from the Stem of Tung Tree (Vernicia fordii)
title_full The First Report on Transgenic Hairy Root Induction from the Stem of Tung Tree (Vernicia fordii)
title_fullStr The First Report on Transgenic Hairy Root Induction from the Stem of Tung Tree (Vernicia fordii)
title_full_unstemmed The First Report on Transgenic Hairy Root Induction from the Stem of Tung Tree (Vernicia fordii)
title_short The First Report on Transgenic Hairy Root Induction from the Stem of Tung Tree (Vernicia fordii)
title_sort first report on transgenic hairy root induction from the stem of tung tree (vernicia fordii)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148109/
https://www.ncbi.nlm.nih.gov/pubmed/35631740
http://dx.doi.org/10.3390/plants11101315
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