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The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations
The ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of sur...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148136/ https://www.ncbi.nlm.nih.gov/pubmed/35628592 http://dx.doi.org/10.3390/ijms23105783 |
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author | Schicht, Martin Riedlová, Kamila Kukulka, Mercedes Li, Wenyue Scheer, Aurelius Garreis, Fabian Jacobi, Christina Paulsen, Friedrich Cwiklik, Lukasz Bräuer, Lars |
author_facet | Schicht, Martin Riedlová, Kamila Kukulka, Mercedes Li, Wenyue Scheer, Aurelius Garreis, Fabian Jacobi, Christina Paulsen, Friedrich Cwiklik, Lukasz Bräuer, Lars |
author_sort | Schicht, Martin |
collection | PubMed |
description | The ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of surfactant protein G (SP-G/SFTA2) with respect to wound healing and surface activity. Bioinformatic, biochemical, and immunological methods were combined to elucidate the role of SP-G in tear film. The results show the presence of SP-G in ocular surface tissues and tear film (TF). Increased expression of SP-G was demonstrated in TF of patients with dry eye disease (DED). Addition of recombinant SP-G in combination with lipids led to an accelerated wound healing of human corneal cells as well as to a reduction of TF surface tension. Molecular modeling of TF suggest that SP-G may regulate tear film surface tension and improve its stability through specific interactions with lipids components of the tear film. In conclusion, SP-G is an ocular surface protein with putative wound healing properties that can also reduce the surface tension of the tear film. |
format | Online Article Text |
id | pubmed-9148136 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91481362022-05-29 The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations Schicht, Martin Riedlová, Kamila Kukulka, Mercedes Li, Wenyue Scheer, Aurelius Garreis, Fabian Jacobi, Christina Paulsen, Friedrich Cwiklik, Lukasz Bräuer, Lars Int J Mol Sci Article The ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of surfactant protein G (SP-G/SFTA2) with respect to wound healing and surface activity. Bioinformatic, biochemical, and immunological methods were combined to elucidate the role of SP-G in tear film. The results show the presence of SP-G in ocular surface tissues and tear film (TF). Increased expression of SP-G was demonstrated in TF of patients with dry eye disease (DED). Addition of recombinant SP-G in combination with lipids led to an accelerated wound healing of human corneal cells as well as to a reduction of TF surface tension. Molecular modeling of TF suggest that SP-G may regulate tear film surface tension and improve its stability through specific interactions with lipids components of the tear film. In conclusion, SP-G is an ocular surface protein with putative wound healing properties that can also reduce the surface tension of the tear film. MDPI 2022-05-21 /pmc/articles/PMC9148136/ /pubmed/35628592 http://dx.doi.org/10.3390/ijms23105783 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Schicht, Martin Riedlová, Kamila Kukulka, Mercedes Li, Wenyue Scheer, Aurelius Garreis, Fabian Jacobi, Christina Paulsen, Friedrich Cwiklik, Lukasz Bräuer, Lars The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations |
title | The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations |
title_full | The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations |
title_fullStr | The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations |
title_full_unstemmed | The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations |
title_short | The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations |
title_sort | potential role of sp-g as surface tension regulator in tear film: from molecular simulations to experimental observations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148136/ https://www.ncbi.nlm.nih.gov/pubmed/35628592 http://dx.doi.org/10.3390/ijms23105783 |
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