Examining Skin Recovery After a 3% Aqueous Hydrogen Peroxide (H(2)O(2)) Treatment Using ATP Biofluorescence

INTRODUCTION: Since its complete mapping, the human skin microbiome has become an important area of research related to skin health. The human skin is populated by an environment of microorganisms, fungi, insects, and viruses that is collectively known as the microbiota, and the complete genomic contribution to the skin is called the microbiome. The terms are different but frequently used interchangeably. Measuring the skin’s microbial diversity can be done, but it is a sophisticated technique that is performed using expensive instruments that can sequence the 16S ribosomal RNA of the microorganisms. Finding more rapid and less costly methods to analyze the changes in the skin’s microbial biome is desirable. METHODS: A study was conducted on thirty (30) inner volar forearms to see if ATP biofluorescence could be employed to examine skin microbial dysbiosis caused by the application of 3% hydrogen peroxide. Fifteen individuals were examined on both arms for a total of thirty inner volar forearms using a Charm Science(®) NovaLum(®) ATP analyzer to examine in a broad sense the skin’s total microbial population and how it is affected after surface treatment with 3% hydrogen peroxide over a 24-hour period. RESULTS: It was found that surface treatment of the skin with three cotton swab applications of 3% hydrogen peroxide five minutes apart was able to statistically significantly suppress the expression of ATP biofluorescence compared against un-swabbed sites and the effects remained significant for six hours following the H(2)O(2) treatment. After 8 hours, and into the 24th hour, the ATP biofluorescence difference returns to non-statistical significance indicating potential return of the stable microbiota. DISCUSSION: Using ATP biofluorescence to detect possible sanitizer-induced microbial dysbiosis may be a rapid way to examine how skin treatments may impact the return of microbially disrupted skin to its normal state and how surface treatments may impact the rate of return to normal after a disruptive event.