Cargando…

Comparative Transcriptome Analysis of Gingival Immune-Mediated Inflammation in Peri-Implantitis and Periodontitis Within the Same Host Environment

OBJECTIVE: This investigation aimed to determine whether and to what extent there are transcriptional differences between periodontitis and peri-implantitis in the same susceptible host. BACKGROUND: As an immune-mediated inflammatory disease resulting in aggressive bone resorption around dental impl...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuan, Shasha, Wang, Cui, Jiang, Wenting, Wei, Yiping, Li, Qingqing, Song, Zhanming, Li, Siqi, Sun, Fei, Liu, Zhongtian, Wang, Ying, Hu, Wenjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148613/
https://www.ncbi.nlm.nih.gov/pubmed/35642216
http://dx.doi.org/10.2147/JIR.S363538
_version_ 1784717070983757824
author Yuan, Shasha
Wang, Cui
Jiang, Wenting
Wei, Yiping
Li, Qingqing
Song, Zhanming
Li, Siqi
Sun, Fei
Liu, Zhongtian
Wang, Ying
Hu, Wenjie
author_facet Yuan, Shasha
Wang, Cui
Jiang, Wenting
Wei, Yiping
Li, Qingqing
Song, Zhanming
Li, Siqi
Sun, Fei
Liu, Zhongtian
Wang, Ying
Hu, Wenjie
author_sort Yuan, Shasha
collection PubMed
description OBJECTIVE: This investigation aimed to determine whether and to what extent there are transcriptional differences between periodontitis and peri-implantitis in the same susceptible host. BACKGROUND: As an immune-mediated inflammatory disease resulting in aggressive bone resorption around dental implants, peri-implantitis constitutes a major threat to dental implants’ long-term success. Compared to periodontitis, its etiological molecular mechanism remains elusive. Currently, there are few investigations on these two diseases at the transcriptional level within the same basal environment. METHODS: Ligature-induced peri-implantitis and periodontitis were generated in the same mice. Gingival tissues of healthy, periodontitis, and peri-implantitis sites from the same oral cavity were collected and used for RNA sequencing. Differentially expressed genes (DEGs) were screened between periodontitis/peri-implantitis and healthy sites. Enrichment analysis of DEGs was performed. The comprehensive immune landscape was annotated by seq-ImmuCC. Hub genes from peri-implantitis-specific DEGs were filtered using the STRING database and Cytoscape. Validation of the selected hub genes was performed on the GEO106090 dataset (gingival tissues from six periodontitis patients, six peri-implantitis patients, and six healthy controls). RESULTS: The results indicated that peri-implantitis and periodontitis exhibited significantly distinct transcriptional signatures, with the complement and coagulation cascade pathways and osteoclast differentiation predominating in peri-implantitis mucosa. Compared with periodontitis, peri-implantitis sites exhibited elevated macrophage proportions and relatively enriched macrophage activation and bone loss. Hub genes were selected, and IL1B, CCL3, and CLEC4E were significantly highly expressed in human peri-implantitis mucosa. CONCLUSION: The study suggests that the interplay between macrophages and bone resorption seems to be more robust than in periodontitis. IL1B, CCL3, and CLEC4E may be considered promising therapeutic targets for peri-implantitis. These critical biological processes and identified genes may contribute to the etiology of peri-implantitis, which is unique from periodontitis. This work may make way for deeper exploration and contribute significantly to the treatment and prevention of peri-implantitis.
format Online
Article
Text
id pubmed-9148613
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-91486132022-05-30 Comparative Transcriptome Analysis of Gingival Immune-Mediated Inflammation in Peri-Implantitis and Periodontitis Within the Same Host Environment Yuan, Shasha Wang, Cui Jiang, Wenting Wei, Yiping Li, Qingqing Song, Zhanming Li, Siqi Sun, Fei Liu, Zhongtian Wang, Ying Hu, Wenjie J Inflamm Res Original Research OBJECTIVE: This investigation aimed to determine whether and to what extent there are transcriptional differences between periodontitis and peri-implantitis in the same susceptible host. BACKGROUND: As an immune-mediated inflammatory disease resulting in aggressive bone resorption around dental implants, peri-implantitis constitutes a major threat to dental implants’ long-term success. Compared to periodontitis, its etiological molecular mechanism remains elusive. Currently, there are few investigations on these two diseases at the transcriptional level within the same basal environment. METHODS: Ligature-induced peri-implantitis and periodontitis were generated in the same mice. Gingival tissues of healthy, periodontitis, and peri-implantitis sites from the same oral cavity were collected and used for RNA sequencing. Differentially expressed genes (DEGs) were screened between periodontitis/peri-implantitis and healthy sites. Enrichment analysis of DEGs was performed. The comprehensive immune landscape was annotated by seq-ImmuCC. Hub genes from peri-implantitis-specific DEGs were filtered using the STRING database and Cytoscape. Validation of the selected hub genes was performed on the GEO106090 dataset (gingival tissues from six periodontitis patients, six peri-implantitis patients, and six healthy controls). RESULTS: The results indicated that peri-implantitis and periodontitis exhibited significantly distinct transcriptional signatures, with the complement and coagulation cascade pathways and osteoclast differentiation predominating in peri-implantitis mucosa. Compared with periodontitis, peri-implantitis sites exhibited elevated macrophage proportions and relatively enriched macrophage activation and bone loss. Hub genes were selected, and IL1B, CCL3, and CLEC4E were significantly highly expressed in human peri-implantitis mucosa. CONCLUSION: The study suggests that the interplay between macrophages and bone resorption seems to be more robust than in periodontitis. IL1B, CCL3, and CLEC4E may be considered promising therapeutic targets for peri-implantitis. These critical biological processes and identified genes may contribute to the etiology of peri-implantitis, which is unique from periodontitis. This work may make way for deeper exploration and contribute significantly to the treatment and prevention of peri-implantitis. Dove 2022-05-25 /pmc/articles/PMC9148613/ /pubmed/35642216 http://dx.doi.org/10.2147/JIR.S363538 Text en © 2022 Yuan et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Yuan, Shasha
Wang, Cui
Jiang, Wenting
Wei, Yiping
Li, Qingqing
Song, Zhanming
Li, Siqi
Sun, Fei
Liu, Zhongtian
Wang, Ying
Hu, Wenjie
Comparative Transcriptome Analysis of Gingival Immune-Mediated Inflammation in Peri-Implantitis and Periodontitis Within the Same Host Environment
title Comparative Transcriptome Analysis of Gingival Immune-Mediated Inflammation in Peri-Implantitis and Periodontitis Within the Same Host Environment
title_full Comparative Transcriptome Analysis of Gingival Immune-Mediated Inflammation in Peri-Implantitis and Periodontitis Within the Same Host Environment
title_fullStr Comparative Transcriptome Analysis of Gingival Immune-Mediated Inflammation in Peri-Implantitis and Periodontitis Within the Same Host Environment
title_full_unstemmed Comparative Transcriptome Analysis of Gingival Immune-Mediated Inflammation in Peri-Implantitis and Periodontitis Within the Same Host Environment
title_short Comparative Transcriptome Analysis of Gingival Immune-Mediated Inflammation in Peri-Implantitis and Periodontitis Within the Same Host Environment
title_sort comparative transcriptome analysis of gingival immune-mediated inflammation in peri-implantitis and periodontitis within the same host environment
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148613/
https://www.ncbi.nlm.nih.gov/pubmed/35642216
http://dx.doi.org/10.2147/JIR.S363538
work_keys_str_mv AT yuanshasha comparativetranscriptomeanalysisofgingivalimmunemediatedinflammationinperiimplantitisandperiodontitiswithinthesamehostenvironment
AT wangcui comparativetranscriptomeanalysisofgingivalimmunemediatedinflammationinperiimplantitisandperiodontitiswithinthesamehostenvironment
AT jiangwenting comparativetranscriptomeanalysisofgingivalimmunemediatedinflammationinperiimplantitisandperiodontitiswithinthesamehostenvironment
AT weiyiping comparativetranscriptomeanalysisofgingivalimmunemediatedinflammationinperiimplantitisandperiodontitiswithinthesamehostenvironment
AT liqingqing comparativetranscriptomeanalysisofgingivalimmunemediatedinflammationinperiimplantitisandperiodontitiswithinthesamehostenvironment
AT songzhanming comparativetranscriptomeanalysisofgingivalimmunemediatedinflammationinperiimplantitisandperiodontitiswithinthesamehostenvironment
AT lisiqi comparativetranscriptomeanalysisofgingivalimmunemediatedinflammationinperiimplantitisandperiodontitiswithinthesamehostenvironment
AT sunfei comparativetranscriptomeanalysisofgingivalimmunemediatedinflammationinperiimplantitisandperiodontitiswithinthesamehostenvironment
AT liuzhongtian comparativetranscriptomeanalysisofgingivalimmunemediatedinflammationinperiimplantitisandperiodontitiswithinthesamehostenvironment
AT wangying comparativetranscriptomeanalysisofgingivalimmunemediatedinflammationinperiimplantitisandperiodontitiswithinthesamehostenvironment
AT huwenjie comparativetranscriptomeanalysisofgingivalimmunemediatedinflammationinperiimplantitisandperiodontitiswithinthesamehostenvironment