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Genome Re-Sequencing Reveals the Host-Specific Origin of Genetic Variation in Magnaporthe Species

Rice blast is caused by Magnaporthe oryzae (M. oryzae), which is considered one of the most serious pathogens of rice around the globe. It causes severe losses owing to its proven capability to disrupt the host resistance. Recently, its invasion of new hosts like the Musa species or banana plants ha...

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Autores principales: Li, Jinbin, Lu, Lin, Wang, Qun, Shi, Zhufeng, Li, Chengyun, Guo, Zhixiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149001/
https://www.ncbi.nlm.nih.gov/pubmed/35651945
http://dx.doi.org/10.3389/fgene.2022.861727
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author Li, Jinbin
Lu, Lin
Wang, Qun
Shi, Zhufeng
Li, Chengyun
Guo, Zhixiang
author_facet Li, Jinbin
Lu, Lin
Wang, Qun
Shi, Zhufeng
Li, Chengyun
Guo, Zhixiang
author_sort Li, Jinbin
collection PubMed
description Rice blast is caused by Magnaporthe oryzae (M. oryzae), which is considered one of the most serious pathogens of rice around the globe. It causes severe losses owing to its proven capability to disrupt the host resistance. Recently, its invasion of new hosts like the Musa species or banana plants has been noticed. To understand the possible level of genetic variation, we sequenced the genomes of eight different isolates of the Magnaporthe species infecting rice, Digitaria (a weed), finger millet, Elusine indica, and banana plants. Comparative genomic analysis of these eight isolates with the previously well-characterized laboratory strain M. oryzae 70-15 was made. The infectivity of the newly isolated strain from Musa species suggested that there is no resistance level in the host plants. The sequence analysis revealed that despite genome similarities, both the banana and Digitaria isolates have relatively larger genome sizes (∼38.2 and 51.1 Mb, respectively) compared to those of the laboratory reference strain M. oryzae 70-15 (∼37 Mb). The gene contraction, expansion, and InDel analysis revealed that during evolution, a higher number of gene insertions and deletions occurred in the blast fungus infecting Digitaria and banana. Furthermore, each genome shared thousands of genes, which suggest their common evolution. Overall, our analysis indicates that higher levels of genes insertion or deletions and gain in the total genome size are important factors in disrupting the host immunity and change in host selection.
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spelling pubmed-91490012022-05-31 Genome Re-Sequencing Reveals the Host-Specific Origin of Genetic Variation in Magnaporthe Species Li, Jinbin Lu, Lin Wang, Qun Shi, Zhufeng Li, Chengyun Guo, Zhixiang Front Genet Genetics Rice blast is caused by Magnaporthe oryzae (M. oryzae), which is considered one of the most serious pathogens of rice around the globe. It causes severe losses owing to its proven capability to disrupt the host resistance. Recently, its invasion of new hosts like the Musa species or banana plants has been noticed. To understand the possible level of genetic variation, we sequenced the genomes of eight different isolates of the Magnaporthe species infecting rice, Digitaria (a weed), finger millet, Elusine indica, and banana plants. Comparative genomic analysis of these eight isolates with the previously well-characterized laboratory strain M. oryzae 70-15 was made. The infectivity of the newly isolated strain from Musa species suggested that there is no resistance level in the host plants. The sequence analysis revealed that despite genome similarities, both the banana and Digitaria isolates have relatively larger genome sizes (∼38.2 and 51.1 Mb, respectively) compared to those of the laboratory reference strain M. oryzae 70-15 (∼37 Mb). The gene contraction, expansion, and InDel analysis revealed that during evolution, a higher number of gene insertions and deletions occurred in the blast fungus infecting Digitaria and banana. Furthermore, each genome shared thousands of genes, which suggest their common evolution. Overall, our analysis indicates that higher levels of genes insertion or deletions and gain in the total genome size are important factors in disrupting the host immunity and change in host selection. Frontiers Media S.A. 2022-05-16 /pmc/articles/PMC9149001/ /pubmed/35651945 http://dx.doi.org/10.3389/fgene.2022.861727 Text en Copyright © 2022 Li, Lu, Wang, Shi, Li and Guo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Li, Jinbin
Lu, Lin
Wang, Qun
Shi, Zhufeng
Li, Chengyun
Guo, Zhixiang
Genome Re-Sequencing Reveals the Host-Specific Origin of Genetic Variation in Magnaporthe Species
title Genome Re-Sequencing Reveals the Host-Specific Origin of Genetic Variation in Magnaporthe Species
title_full Genome Re-Sequencing Reveals the Host-Specific Origin of Genetic Variation in Magnaporthe Species
title_fullStr Genome Re-Sequencing Reveals the Host-Specific Origin of Genetic Variation in Magnaporthe Species
title_full_unstemmed Genome Re-Sequencing Reveals the Host-Specific Origin of Genetic Variation in Magnaporthe Species
title_short Genome Re-Sequencing Reveals the Host-Specific Origin of Genetic Variation in Magnaporthe Species
title_sort genome re-sequencing reveals the host-specific origin of genetic variation in magnaporthe species
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149001/
https://www.ncbi.nlm.nih.gov/pubmed/35651945
http://dx.doi.org/10.3389/fgene.2022.861727
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