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Renal Nerve Activity and Arterial Depressor Responses Induced by Neuromodulation of the Deep Peroneal Nerve in Spontaneously Hypertensive Rats

Hypertension is a main cause of death in the United States with more than 103 million adults affected. While pharmacological treatments are effective, blood pressure (BP) remains uncontrolled in 50–60% of resistant hypertensive subjects. Using a custom-wired miniature electrode, we previously report...

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Autores principales: Gonzalez-Gonzalez, Maria Alejandra, Romero, Kevin, Beitter, John, Lloyd, David, Lam, Danny V., Hernandez-Reynoso, Ana Guadalupe, Kanneganti, Aswini, Kim, Han-Kyul, Bjune, Caroline K., Smith, Scott, Vongpatanasin, Wanpen, Romero-Ortega, Mario I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149221/
https://www.ncbi.nlm.nih.gov/pubmed/35651628
http://dx.doi.org/10.3389/fnins.2022.726467
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author Gonzalez-Gonzalez, Maria Alejandra
Romero, Kevin
Beitter, John
Lloyd, David
Lam, Danny V.
Hernandez-Reynoso, Ana Guadalupe
Kanneganti, Aswini
Kim, Han-Kyul
Bjune, Caroline K.
Smith, Scott
Vongpatanasin, Wanpen
Romero-Ortega, Mario I.
author_facet Gonzalez-Gonzalez, Maria Alejandra
Romero, Kevin
Beitter, John
Lloyd, David
Lam, Danny V.
Hernandez-Reynoso, Ana Guadalupe
Kanneganti, Aswini
Kim, Han-Kyul
Bjune, Caroline K.
Smith, Scott
Vongpatanasin, Wanpen
Romero-Ortega, Mario I.
author_sort Gonzalez-Gonzalez, Maria Alejandra
collection PubMed
description Hypertension is a main cause of death in the United States with more than 103 million adults affected. While pharmacological treatments are effective, blood pressure (BP) remains uncontrolled in 50–60% of resistant hypertensive subjects. Using a custom-wired miniature electrode, we previously reported that deep peroneal nerve stimulation (DPNS) elicited acute cardiovascular depressor responses in anesthetized spontaneously hypertensive rats (SHRs). Here, we further study this effect by implementing a wireless system and exploring different stimulation parameters to achieve a maximum depressor response. Our results indicate that DPNS consistently induces a reduction in BP and suggests that renal sympathetic nerve activity (RSNA) is altered by this bioelectronic treatment. To test the acute effect of DPNS in awake animals, we developed a novel miniaturized wireless microchannel electrode (w-μCE), with a Z-shaped microchannel through which the target nerves slide and lock into the recording/stimulation chamber. Animals implanted with w-μCE and BP telemetry systems for 3 weeks showed an average BP of 150 ± 14 mmHg, which was reduced significantly by an active DPNS session to 135 ± 8 mmHg (p < 0.04), but not in sham-treated animals. The depressor response in animals with an active w-μCE was progressively returned to baseline levels 14 min later (164 ± 26 mmHg). This depressor response was confirmed in restrained fully awake animals that received DPNS for 10 days, where tail-cuff BP measurements showed that systolic BP in SHR lowered 10% at 1 h and 16% 2 h after the DPNS when compared to the post-implantation baseline. Together, these results support the use of DPN neuromodulation as a possible strategy to lower BP in drug-resistant hypertension.
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spelling pubmed-91492212022-05-31 Renal Nerve Activity and Arterial Depressor Responses Induced by Neuromodulation of the Deep Peroneal Nerve in Spontaneously Hypertensive Rats Gonzalez-Gonzalez, Maria Alejandra Romero, Kevin Beitter, John Lloyd, David Lam, Danny V. Hernandez-Reynoso, Ana Guadalupe Kanneganti, Aswini Kim, Han-Kyul Bjune, Caroline K. Smith, Scott Vongpatanasin, Wanpen Romero-Ortega, Mario I. Front Neurosci Neuroscience Hypertension is a main cause of death in the United States with more than 103 million adults affected. While pharmacological treatments are effective, blood pressure (BP) remains uncontrolled in 50–60% of resistant hypertensive subjects. Using a custom-wired miniature electrode, we previously reported that deep peroneal nerve stimulation (DPNS) elicited acute cardiovascular depressor responses in anesthetized spontaneously hypertensive rats (SHRs). Here, we further study this effect by implementing a wireless system and exploring different stimulation parameters to achieve a maximum depressor response. Our results indicate that DPNS consistently induces a reduction in BP and suggests that renal sympathetic nerve activity (RSNA) is altered by this bioelectronic treatment. To test the acute effect of DPNS in awake animals, we developed a novel miniaturized wireless microchannel electrode (w-μCE), with a Z-shaped microchannel through which the target nerves slide and lock into the recording/stimulation chamber. Animals implanted with w-μCE and BP telemetry systems for 3 weeks showed an average BP of 150 ± 14 mmHg, which was reduced significantly by an active DPNS session to 135 ± 8 mmHg (p < 0.04), but not in sham-treated animals. The depressor response in animals with an active w-μCE was progressively returned to baseline levels 14 min later (164 ± 26 mmHg). This depressor response was confirmed in restrained fully awake animals that received DPNS for 10 days, where tail-cuff BP measurements showed that systolic BP in SHR lowered 10% at 1 h and 16% 2 h after the DPNS when compared to the post-implantation baseline. Together, these results support the use of DPN neuromodulation as a possible strategy to lower BP in drug-resistant hypertension. Frontiers Media S.A. 2022-05-16 /pmc/articles/PMC9149221/ /pubmed/35651628 http://dx.doi.org/10.3389/fnins.2022.726467 Text en Copyright © 2022 Gonzalez-Gonzalez, Romero, Beitter, Lloyd, Lam, Hernandez-Reynoso, Kanneganti, Kim, Bjune, Smith, Vongpatanasin and Romero-Ortega. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Gonzalez-Gonzalez, Maria Alejandra
Romero, Kevin
Beitter, John
Lloyd, David
Lam, Danny V.
Hernandez-Reynoso, Ana Guadalupe
Kanneganti, Aswini
Kim, Han-Kyul
Bjune, Caroline K.
Smith, Scott
Vongpatanasin, Wanpen
Romero-Ortega, Mario I.
Renal Nerve Activity and Arterial Depressor Responses Induced by Neuromodulation of the Deep Peroneal Nerve in Spontaneously Hypertensive Rats
title Renal Nerve Activity and Arterial Depressor Responses Induced by Neuromodulation of the Deep Peroneal Nerve in Spontaneously Hypertensive Rats
title_full Renal Nerve Activity and Arterial Depressor Responses Induced by Neuromodulation of the Deep Peroneal Nerve in Spontaneously Hypertensive Rats
title_fullStr Renal Nerve Activity and Arterial Depressor Responses Induced by Neuromodulation of the Deep Peroneal Nerve in Spontaneously Hypertensive Rats
title_full_unstemmed Renal Nerve Activity and Arterial Depressor Responses Induced by Neuromodulation of the Deep Peroneal Nerve in Spontaneously Hypertensive Rats
title_short Renal Nerve Activity and Arterial Depressor Responses Induced by Neuromodulation of the Deep Peroneal Nerve in Spontaneously Hypertensive Rats
title_sort renal nerve activity and arterial depressor responses induced by neuromodulation of the deep peroneal nerve in spontaneously hypertensive rats
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149221/
https://www.ncbi.nlm.nih.gov/pubmed/35651628
http://dx.doi.org/10.3389/fnins.2022.726467
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