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Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Aluminum and Iron Toxicities in Rice

Metal toxicities can be detrimental to a plant health, as well as to the health of animals and humans that consume such plants. Metal content of plants can be analyzed using colorimetric, atomic absorption- or mass spectroscopy-based methods. However, these techniques are destructive, costly and lab...

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Autores principales: Higgins, Samantha, Biswas, Sudip, Goff, Nicolas K., Septiningsih, Endang M., Kurouski, Dmitry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149412/
https://www.ncbi.nlm.nih.gov/pubmed/35651767
http://dx.doi.org/10.3389/fpls.2022.754735
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author Higgins, Samantha
Biswas, Sudip
Goff, Nicolas K.
Septiningsih, Endang M.
Kurouski, Dmitry
author_facet Higgins, Samantha
Biswas, Sudip
Goff, Nicolas K.
Septiningsih, Endang M.
Kurouski, Dmitry
author_sort Higgins, Samantha
collection PubMed
description Metal toxicities can be detrimental to a plant health, as well as to the health of animals and humans that consume such plants. Metal content of plants can be analyzed using colorimetric, atomic absorption- or mass spectroscopy-based methods. However, these techniques are destructive, costly and laborious. In the current study, we investigate the potential of Raman spectroscopy (RS), a modern spectroscopic technique, for detection and identification of metal toxicities in rice. We modeled medium and high levels of iron and aluminum toxicities in hydroponically grown plants. Spectroscopic analyses of their leaves showed that both iron and aluminum toxicities can be detected and identified with ∼100% accuracy as early as day 2 after the stress initiation. We also showed that diagnostics accuracy was very high not only on early, but also on middle (day 4–day 8) and late (day 10–day 14) stages of the stress development. Importantly this approach only requires an acquisition time of 1 s; it is non-invasive and non-destructive to plants. Our findings suggest that if implemented in farming, RS can enable pre-symptomatic detection and identification of metallic toxins that would lead to faster recovery of crops and prevent further damage.
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spelling pubmed-91494122022-05-31 Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Aluminum and Iron Toxicities in Rice Higgins, Samantha Biswas, Sudip Goff, Nicolas K. Septiningsih, Endang M. Kurouski, Dmitry Front Plant Sci Plant Science Metal toxicities can be detrimental to a plant health, as well as to the health of animals and humans that consume such plants. Metal content of plants can be analyzed using colorimetric, atomic absorption- or mass spectroscopy-based methods. However, these techniques are destructive, costly and laborious. In the current study, we investigate the potential of Raman spectroscopy (RS), a modern spectroscopic technique, for detection and identification of metal toxicities in rice. We modeled medium and high levels of iron and aluminum toxicities in hydroponically grown plants. Spectroscopic analyses of their leaves showed that both iron and aluminum toxicities can be detected and identified with ∼100% accuracy as early as day 2 after the stress initiation. We also showed that diagnostics accuracy was very high not only on early, but also on middle (day 4–day 8) and late (day 10–day 14) stages of the stress development. Importantly this approach only requires an acquisition time of 1 s; it is non-invasive and non-destructive to plants. Our findings suggest that if implemented in farming, RS can enable pre-symptomatic detection and identification of metallic toxins that would lead to faster recovery of crops and prevent further damage. Frontiers Media S.A. 2022-05-16 /pmc/articles/PMC9149412/ /pubmed/35651767 http://dx.doi.org/10.3389/fpls.2022.754735 Text en Copyright © 2022 Higgins, Biswas, Goff, Septiningsih and Kurouski. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Higgins, Samantha
Biswas, Sudip
Goff, Nicolas K.
Septiningsih, Endang M.
Kurouski, Dmitry
Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Aluminum and Iron Toxicities in Rice
title Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Aluminum and Iron Toxicities in Rice
title_full Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Aluminum and Iron Toxicities in Rice
title_fullStr Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Aluminum and Iron Toxicities in Rice
title_full_unstemmed Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Aluminum and Iron Toxicities in Rice
title_short Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Aluminum and Iron Toxicities in Rice
title_sort raman spectroscopy enables non-invasive and confirmatory diagnostics of aluminum and iron toxicities in rice
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149412/
https://www.ncbi.nlm.nih.gov/pubmed/35651767
http://dx.doi.org/10.3389/fpls.2022.754735
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