Cargando…

Exogenous Melatonin Modulates Physiological Response to Nitrogen and Improves Yield in Nitrogen-Deficient Soybean (Glycine max L. Merr.)

Melatonin (MT) is a key plant growth regulator. To investigate its effect at different growth stages on the yield of soybean under nitrogen deficiency, 100 μM MT was applied to soybean supplemented with zero nitrogen (0N), low nitrogen (LN), and control nitrogen (CK) levels, during the plant vegetat...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Huamei, Ren, Chunyuan, Cao, Liang, Zhao, Qiang, Jin, Xijun, Wang, Mengxue, Zhang, Mingcong, Yu, Gaobo, Zhang, Yuxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149585/
https://www.ncbi.nlm.nih.gov/pubmed/35651760
http://dx.doi.org/10.3389/fpls.2022.865758
_version_ 1784717232797908992
author Wang, Huamei
Ren, Chunyuan
Cao, Liang
Zhao, Qiang
Jin, Xijun
Wang, Mengxue
Zhang, Mingcong
Yu, Gaobo
Zhang, Yuxian
author_facet Wang, Huamei
Ren, Chunyuan
Cao, Liang
Zhao, Qiang
Jin, Xijun
Wang, Mengxue
Zhang, Mingcong
Yu, Gaobo
Zhang, Yuxian
author_sort Wang, Huamei
collection PubMed
description Melatonin (MT) is a key plant growth regulator. To investigate its effect at different growth stages on the yield of soybean under nitrogen deficiency, 100 μM MT was applied to soybean supplemented with zero nitrogen (0N), low nitrogen (LN), and control nitrogen (CK) levels, during the plant vegetative growth (V3) and filling (R5) stages. This study revealed that the application of MT mainly enhanced the nitrogen fixation of plants by increasing the root nodule number and provided more substrates for glutamine synthetase (GS) under 0N supply. However, under the LN supply, more ammonium was assimilated through the direct promotion of nitrate reductase (NR) activity by MT. MT enhanced the activity of ammonium-assimilation-related enzymes, such as GOGAT and GDH, and the expression of their coding genes, promoted the synthesis of chlorophyll and amino acids, and increased the photosynthetic capacity under nitrogen deficiency. Exogenous MT directly upregulated the expression of genes involved in the photosynthetic system and stimulated dry-matter accumulation. Thus, MT alleviated the inhibitory effect of nitrogen deficiency on soybean yield. This mitigation effect was better when MT was applied at the V3 stage, and the seed weight per plant increased by 16.69 and 12.20% at 0N and LN levels, respectively. The results of this study provide a new theoretical basis to apply MT in agriculture to improve the resilience of soybean plants to low nitrogen availability.
format Online
Article
Text
id pubmed-9149585
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91495852022-05-31 Exogenous Melatonin Modulates Physiological Response to Nitrogen and Improves Yield in Nitrogen-Deficient Soybean (Glycine max L. Merr.) Wang, Huamei Ren, Chunyuan Cao, Liang Zhao, Qiang Jin, Xijun Wang, Mengxue Zhang, Mingcong Yu, Gaobo Zhang, Yuxian Front Plant Sci Plant Science Melatonin (MT) is a key plant growth regulator. To investigate its effect at different growth stages on the yield of soybean under nitrogen deficiency, 100 μM MT was applied to soybean supplemented with zero nitrogen (0N), low nitrogen (LN), and control nitrogen (CK) levels, during the plant vegetative growth (V3) and filling (R5) stages. This study revealed that the application of MT mainly enhanced the nitrogen fixation of plants by increasing the root nodule number and provided more substrates for glutamine synthetase (GS) under 0N supply. However, under the LN supply, more ammonium was assimilated through the direct promotion of nitrate reductase (NR) activity by MT. MT enhanced the activity of ammonium-assimilation-related enzymes, such as GOGAT and GDH, and the expression of their coding genes, promoted the synthesis of chlorophyll and amino acids, and increased the photosynthetic capacity under nitrogen deficiency. Exogenous MT directly upregulated the expression of genes involved in the photosynthetic system and stimulated dry-matter accumulation. Thus, MT alleviated the inhibitory effect of nitrogen deficiency on soybean yield. This mitigation effect was better when MT was applied at the V3 stage, and the seed weight per plant increased by 16.69 and 12.20% at 0N and LN levels, respectively. The results of this study provide a new theoretical basis to apply MT in agriculture to improve the resilience of soybean plants to low nitrogen availability. Frontiers Media S.A. 2022-05-16 /pmc/articles/PMC9149585/ /pubmed/35651760 http://dx.doi.org/10.3389/fpls.2022.865758 Text en Copyright © 2022 Wang, Ren, Cao, Zhao, Jin, Wang, Zhang, Yu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Huamei
Ren, Chunyuan
Cao, Liang
Zhao, Qiang
Jin, Xijun
Wang, Mengxue
Zhang, Mingcong
Yu, Gaobo
Zhang, Yuxian
Exogenous Melatonin Modulates Physiological Response to Nitrogen and Improves Yield in Nitrogen-Deficient Soybean (Glycine max L. Merr.)
title Exogenous Melatonin Modulates Physiological Response to Nitrogen and Improves Yield in Nitrogen-Deficient Soybean (Glycine max L. Merr.)
title_full Exogenous Melatonin Modulates Physiological Response to Nitrogen and Improves Yield in Nitrogen-Deficient Soybean (Glycine max L. Merr.)
title_fullStr Exogenous Melatonin Modulates Physiological Response to Nitrogen and Improves Yield in Nitrogen-Deficient Soybean (Glycine max L. Merr.)
title_full_unstemmed Exogenous Melatonin Modulates Physiological Response to Nitrogen and Improves Yield in Nitrogen-Deficient Soybean (Glycine max L. Merr.)
title_short Exogenous Melatonin Modulates Physiological Response to Nitrogen and Improves Yield in Nitrogen-Deficient Soybean (Glycine max L. Merr.)
title_sort exogenous melatonin modulates physiological response to nitrogen and improves yield in nitrogen-deficient soybean (glycine max l. merr.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149585/
https://www.ncbi.nlm.nih.gov/pubmed/35651760
http://dx.doi.org/10.3389/fpls.2022.865758
work_keys_str_mv AT wanghuamei exogenousmelatoninmodulatesphysiologicalresponsetonitrogenandimprovesyieldinnitrogendeficientsoybeanglycinemaxlmerr
AT renchunyuan exogenousmelatoninmodulatesphysiologicalresponsetonitrogenandimprovesyieldinnitrogendeficientsoybeanglycinemaxlmerr
AT caoliang exogenousmelatoninmodulatesphysiologicalresponsetonitrogenandimprovesyieldinnitrogendeficientsoybeanglycinemaxlmerr
AT zhaoqiang exogenousmelatoninmodulatesphysiologicalresponsetonitrogenandimprovesyieldinnitrogendeficientsoybeanglycinemaxlmerr
AT jinxijun exogenousmelatoninmodulatesphysiologicalresponsetonitrogenandimprovesyieldinnitrogendeficientsoybeanglycinemaxlmerr
AT wangmengxue exogenousmelatoninmodulatesphysiologicalresponsetonitrogenandimprovesyieldinnitrogendeficientsoybeanglycinemaxlmerr
AT zhangmingcong exogenousmelatoninmodulatesphysiologicalresponsetonitrogenandimprovesyieldinnitrogendeficientsoybeanglycinemaxlmerr
AT yugaobo exogenousmelatoninmodulatesphysiologicalresponsetonitrogenandimprovesyieldinnitrogendeficientsoybeanglycinemaxlmerr
AT zhangyuxian exogenousmelatoninmodulatesphysiologicalresponsetonitrogenandimprovesyieldinnitrogendeficientsoybeanglycinemaxlmerr