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Immune Reconstitution in Pediatric Aplastic Anemia after Allogeneic Hematopoietic Stem-cell Transplantation

Background: Previous studies had revealed that immune reconstitution (IR) after allogeneic hematopoietic stem-cell transplantation (allo-HSCT) affected the clinical prognosis of patients. However, few studies were based on pediatric patients and patients with aplastic anemia (AA). The purpose of thi...

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Autores principales: Wang, Jiayu, Yuan, Meng, Zhu, Guanghua, Wu, Runhui, Jia, Chenguang, Wang, Bin, Zheng, Jie, Ma, Jie, Qin, Maoquan, Li, Sidan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149648/
https://www.ncbi.nlm.nih.gov/pubmed/35693743
http://dx.doi.org/10.7150/ijms.70146
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author Wang, Jiayu
Yuan, Meng
Zhu, Guanghua
Wu, Runhui
Jia, Chenguang
Wang, Bin
Zheng, Jie
Ma, Jie
Qin, Maoquan
Li, Sidan
author_facet Wang, Jiayu
Yuan, Meng
Zhu, Guanghua
Wu, Runhui
Jia, Chenguang
Wang, Bin
Zheng, Jie
Ma, Jie
Qin, Maoquan
Li, Sidan
author_sort Wang, Jiayu
collection PubMed
description Background: Previous studies had revealed that immune reconstitution (IR) after allogeneic hematopoietic stem-cell transplantation (allo-HSCT) affected the clinical prognosis of patients. However, few studies were based on pediatric patients and patients with aplastic anemia (AA). The purpose of this research was to analyze IR of pediatric AA after HSCT and further explore its clinical prognostic value. Methods: The whole of 61 pediatric patients with AA who underwent HSCT were enrolled. Lymphocyte subsets count in peripheral blood, CD4(+)/CD8(+) T cell ratio, and serum concentration of immunoglobulins were detected using flow cytometry at regular intervals after HSCT. Results: Innate immunity recovered faster than adaptive immunity, T lymphocytes recovered faster than B lymphocytes. The number of transfused CD34(+) cells and the implantation time of ANC significantly affected the early rapid IR of CD3(+) T cells. The degree of HLA site coincidence significantly affected the early rapid IR of CD19(+) B cells. The number of transfused MNC and CD34(+) cells significantly affected the early rapid IR of CD56(+) NK cells. The overall survival (OS) and failure-free survival (FFS) of CD56(+) NK cells in early rapid IR group were higher than those in non-IR group. The CD3(+) T cell early rapid IR group and CD8(+) T cell early rapid IR group had higher OS than the non-IR group. Conclusion: Early rapid IR after HSCT is a good predictor of clinical prognosis in children with AA. This study provides a reasonable prediction for early rapid IR, which may improve clinical outcomes of children.
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spelling pubmed-91496482022-06-10 Immune Reconstitution in Pediatric Aplastic Anemia after Allogeneic Hematopoietic Stem-cell Transplantation Wang, Jiayu Yuan, Meng Zhu, Guanghua Wu, Runhui Jia, Chenguang Wang, Bin Zheng, Jie Ma, Jie Qin, Maoquan Li, Sidan Int J Med Sci Research Paper Background: Previous studies had revealed that immune reconstitution (IR) after allogeneic hematopoietic stem-cell transplantation (allo-HSCT) affected the clinical prognosis of patients. However, few studies were based on pediatric patients and patients with aplastic anemia (AA). The purpose of this research was to analyze IR of pediatric AA after HSCT and further explore its clinical prognostic value. Methods: The whole of 61 pediatric patients with AA who underwent HSCT were enrolled. Lymphocyte subsets count in peripheral blood, CD4(+)/CD8(+) T cell ratio, and serum concentration of immunoglobulins were detected using flow cytometry at regular intervals after HSCT. Results: Innate immunity recovered faster than adaptive immunity, T lymphocytes recovered faster than B lymphocytes. The number of transfused CD34(+) cells and the implantation time of ANC significantly affected the early rapid IR of CD3(+) T cells. The degree of HLA site coincidence significantly affected the early rapid IR of CD19(+) B cells. The number of transfused MNC and CD34(+) cells significantly affected the early rapid IR of CD56(+) NK cells. The overall survival (OS) and failure-free survival (FFS) of CD56(+) NK cells in early rapid IR group were higher than those in non-IR group. The CD3(+) T cell early rapid IR group and CD8(+) T cell early rapid IR group had higher OS than the non-IR group. Conclusion: Early rapid IR after HSCT is a good predictor of clinical prognosis in children with AA. This study provides a reasonable prediction for early rapid IR, which may improve clinical outcomes of children. Ivyspring International Publisher 2022-05-01 /pmc/articles/PMC9149648/ /pubmed/35693743 http://dx.doi.org/10.7150/ijms.70146 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Wang, Jiayu
Yuan, Meng
Zhu, Guanghua
Wu, Runhui
Jia, Chenguang
Wang, Bin
Zheng, Jie
Ma, Jie
Qin, Maoquan
Li, Sidan
Immune Reconstitution in Pediatric Aplastic Anemia after Allogeneic Hematopoietic Stem-cell Transplantation
title Immune Reconstitution in Pediatric Aplastic Anemia after Allogeneic Hematopoietic Stem-cell Transplantation
title_full Immune Reconstitution in Pediatric Aplastic Anemia after Allogeneic Hematopoietic Stem-cell Transplantation
title_fullStr Immune Reconstitution in Pediatric Aplastic Anemia after Allogeneic Hematopoietic Stem-cell Transplantation
title_full_unstemmed Immune Reconstitution in Pediatric Aplastic Anemia after Allogeneic Hematopoietic Stem-cell Transplantation
title_short Immune Reconstitution in Pediatric Aplastic Anemia after Allogeneic Hematopoietic Stem-cell Transplantation
title_sort immune reconstitution in pediatric aplastic anemia after allogeneic hematopoietic stem-cell transplantation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149648/
https://www.ncbi.nlm.nih.gov/pubmed/35693743
http://dx.doi.org/10.7150/ijms.70146
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