Antibody Light Chains: Key to Increased Monoclonal Antibody Yields in Expi293 Cells?

When constructing isogenic recombinant IgM–IgG pairs, we discovered that μ heavy chains strongly prefer partnering with λ light chains for optimal IgM expression in transiently cotransfected Expi293 cells. When μ chains were paired with κ light chains, IgM yields were low but increased by logs—up to...

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Detalles Bibliográficos
Autores principales: Gong, Siqi, Gautam, Seijal, Coneglio, Joshua D., Scinto, Hanna B., Ruprecht, Ruth M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149950/
https://www.ncbi.nlm.nih.gov/pubmed/35645210
http://dx.doi.org/10.3390/antib11020037

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149950/
https://www.ncbi.nlm.nih.gov/pubmed/35645210
http://dx.doi.org/10.3390/antib11020037

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