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Decellularization of Porcine Carotid Arteries: From the Vessel to the High-Quality Scaffold in Five Hours
The use of biologically derived vessels as small-diameter vascular grafts in vascular diseases is currently intensely studied. Vessel decellularization provides a biocompatible scaffold with very low immunogenicity that avoids immunosuppression after transplantation. Good scaffold preservation is im...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150822/ https://www.ncbi.nlm.nih.gov/pubmed/35651544 http://dx.doi.org/10.3389/fbioe.2022.833244 |
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author | Massaro, Maria Stefania Kochová, Petra Pálek, Richard Rosendorf, Jáchym Červenková, Lenka Dahmen, Uta Liška, Václav Moulisová, Vladimíra |
author_facet | Massaro, Maria Stefania Kochová, Petra Pálek, Richard Rosendorf, Jáchym Červenková, Lenka Dahmen, Uta Liška, Václav Moulisová, Vladimíra |
author_sort | Massaro, Maria Stefania |
collection | PubMed |
description | The use of biologically derived vessels as small-diameter vascular grafts in vascular diseases is currently intensely studied. Vessel decellularization provides a biocompatible scaffold with very low immunogenicity that avoids immunosuppression after transplantation. Good scaffold preservation is important as it facilitates successful cell repopulation. In addition, mechanical characteristics have to be carefully evaluated when the graft is intended to be used as an artery due to the high pressures the vessel is subjected to. Here, we present a new and fast decellularization protocol for porcine carotid arteries, followed by investigation of the quality of obtained vessel scaffolds in terms of maintenance of important extracellular matrix components, mechanical resistance, and compatibility with human endothelial cells. Our results evidence that our decellularization protocol minimally alters both the presence of scaffold proteins and their mechanical behavior and human endothelial cells could adhere to the scaffold in vitro. We conclude that if a suitable protocol is used, a high-quality decellularized arterial scaffold of non-human origin can be promptly obtained, having a great potential to be recellularized and used as an arterial graft in transplantation medicine. |
format | Online Article Text |
id | pubmed-9150822 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91508222022-05-31 Decellularization of Porcine Carotid Arteries: From the Vessel to the High-Quality Scaffold in Five Hours Massaro, Maria Stefania Kochová, Petra Pálek, Richard Rosendorf, Jáchym Červenková, Lenka Dahmen, Uta Liška, Václav Moulisová, Vladimíra Front Bioeng Biotechnol Bioengineering and Biotechnology The use of biologically derived vessels as small-diameter vascular grafts in vascular diseases is currently intensely studied. Vessel decellularization provides a biocompatible scaffold with very low immunogenicity that avoids immunosuppression after transplantation. Good scaffold preservation is important as it facilitates successful cell repopulation. In addition, mechanical characteristics have to be carefully evaluated when the graft is intended to be used as an artery due to the high pressures the vessel is subjected to. Here, we present a new and fast decellularization protocol for porcine carotid arteries, followed by investigation of the quality of obtained vessel scaffolds in terms of maintenance of important extracellular matrix components, mechanical resistance, and compatibility with human endothelial cells. Our results evidence that our decellularization protocol minimally alters both the presence of scaffold proteins and their mechanical behavior and human endothelial cells could adhere to the scaffold in vitro. We conclude that if a suitable protocol is used, a high-quality decellularized arterial scaffold of non-human origin can be promptly obtained, having a great potential to be recellularized and used as an arterial graft in transplantation medicine. Frontiers Media S.A. 2022-05-16 /pmc/articles/PMC9150822/ /pubmed/35651544 http://dx.doi.org/10.3389/fbioe.2022.833244 Text en Copyright © 2022 Massaro, Kochová, Pálek, Rosendorf, Červenková, Dahmen, Liška and Moulisová. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Massaro, Maria Stefania Kochová, Petra Pálek, Richard Rosendorf, Jáchym Červenková, Lenka Dahmen, Uta Liška, Václav Moulisová, Vladimíra Decellularization of Porcine Carotid Arteries: From the Vessel to the High-Quality Scaffold in Five Hours |
title | Decellularization of Porcine Carotid Arteries: From the Vessel to the High-Quality Scaffold in Five Hours |
title_full | Decellularization of Porcine Carotid Arteries: From the Vessel to the High-Quality Scaffold in Five Hours |
title_fullStr | Decellularization of Porcine Carotid Arteries: From the Vessel to the High-Quality Scaffold in Five Hours |
title_full_unstemmed | Decellularization of Porcine Carotid Arteries: From the Vessel to the High-Quality Scaffold in Five Hours |
title_short | Decellularization of Porcine Carotid Arteries: From the Vessel to the High-Quality Scaffold in Five Hours |
title_sort | decellularization of porcine carotid arteries: from the vessel to the high-quality scaffold in five hours |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150822/ https://www.ncbi.nlm.nih.gov/pubmed/35651544 http://dx.doi.org/10.3389/fbioe.2022.833244 |
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