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Scleral Cross-Linking in Form-Deprivation Myopic Guinea Pig Eyes Leads to Glaucomatous Changes

PURPOSE: To investigate the potential glaucomatous changes caused by scleral cross-linking (CXL) in a guinea pig form-deprivation (FD) myopia model. METHODS: Eighty 4-week-old tricolor guinea pigs were divided into four groups: FD only, genipin CXL only, FD plus CXL, and control. Refractive error, a...

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Autores principales: Guo, Lei, Hua, Rui, Zhang, Xinxin, Yan, Ting Yu, Tong, Yang, Zhao, Xin, Chen, Shi Chao, Wang, Moying, Bressler, Neil M., Kong, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150827/
https://www.ncbi.nlm.nih.gov/pubmed/35594036
http://dx.doi.org/10.1167/iovs.63.5.24
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author Guo, Lei
Hua, Rui
Zhang, Xinxin
Yan, Ting Yu
Tong, Yang
Zhao, Xin
Chen, Shi Chao
Wang, Moying
Bressler, Neil M.
Kong, Jun
author_facet Guo, Lei
Hua, Rui
Zhang, Xinxin
Yan, Ting Yu
Tong, Yang
Zhao, Xin
Chen, Shi Chao
Wang, Moying
Bressler, Neil M.
Kong, Jun
author_sort Guo, Lei
collection PubMed
description PURPOSE: To investigate the potential glaucomatous changes caused by scleral cross-linking (CXL) in a guinea pig form-deprivation (FD) myopia model. METHODS: Eighty 4-week-old tricolor guinea pigs were divided into four groups: FD only, genipin CXL only, FD plus CXL, and control. Refractive error, axial length (AL), intraocular pressure (IOP), and structural and vasculature optic disc changes in optical coherence tomography (OCT) and OCT angiography (OCTA) were measured at baseline and day 21. CXL efficacy was evaluated by scleral rigidity Young's modulus values. Histological and molecular changes in the anterior chamber angle, retina, and sclera were assessed. RESULTS: Baseline parameters were similar among groups (P > 0.05). The FD plus CXL group at day 21 had the least increase of AL (0.14 ± 0.08 mm) and highest IOP elevation (31.5 ± 3.6 mmHg) compared with the FD-only group (AL: 0.68 ± 0.17 mm; IOP: 22.2 ± 2.6 mmHg) and the control group (AL: 0.24 ± 0.09 mm; IOP: 17.4 ± 1.8 mmHg) (all P < 0.001). OCT and OCTA parameters of the optic disc in the FD plus CXL group at day 21 showed glaucomatous changes and decreased blood flow signals. Sclera rigidity increased in the CXL and FD plus CXL groups. Advanced glycation end products deposited extensively in the retina, choroid, and sclera of FD plus CXL eyes. CONCLUSIONS: CXL causes increased IOP and subsequent optic disc, anterior segment, and scleral changes while inhibiting myopic progression and axial elongation in FD guinea pig eyes. Therefore, applying CXL to control myopia raises safety concerns.
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spelling pubmed-91508272022-05-31 Scleral Cross-Linking in Form-Deprivation Myopic Guinea Pig Eyes Leads to Glaucomatous Changes Guo, Lei Hua, Rui Zhang, Xinxin Yan, Ting Yu Tong, Yang Zhao, Xin Chen, Shi Chao Wang, Moying Bressler, Neil M. Kong, Jun Invest Ophthalmol Vis Sci Anatomy and Pathology/Oncology PURPOSE: To investigate the potential glaucomatous changes caused by scleral cross-linking (CXL) in a guinea pig form-deprivation (FD) myopia model. METHODS: Eighty 4-week-old tricolor guinea pigs were divided into four groups: FD only, genipin CXL only, FD plus CXL, and control. Refractive error, axial length (AL), intraocular pressure (IOP), and structural and vasculature optic disc changes in optical coherence tomography (OCT) and OCT angiography (OCTA) were measured at baseline and day 21. CXL efficacy was evaluated by scleral rigidity Young's modulus values. Histological and molecular changes in the anterior chamber angle, retina, and sclera were assessed. RESULTS: Baseline parameters were similar among groups (P > 0.05). The FD plus CXL group at day 21 had the least increase of AL (0.14 ± 0.08 mm) and highest IOP elevation (31.5 ± 3.6 mmHg) compared with the FD-only group (AL: 0.68 ± 0.17 mm; IOP: 22.2 ± 2.6 mmHg) and the control group (AL: 0.24 ± 0.09 mm; IOP: 17.4 ± 1.8 mmHg) (all P < 0.001). OCT and OCTA parameters of the optic disc in the FD plus CXL group at day 21 showed glaucomatous changes and decreased blood flow signals. Sclera rigidity increased in the CXL and FD plus CXL groups. Advanced glycation end products deposited extensively in the retina, choroid, and sclera of FD plus CXL eyes. CONCLUSIONS: CXL causes increased IOP and subsequent optic disc, anterior segment, and scleral changes while inhibiting myopic progression and axial elongation in FD guinea pig eyes. Therefore, applying CXL to control myopia raises safety concerns. The Association for Research in Vision and Ophthalmology 2022-05-20 /pmc/articles/PMC9150827/ /pubmed/35594036 http://dx.doi.org/10.1167/iovs.63.5.24 Text en Copyright 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Anatomy and Pathology/Oncology
Guo, Lei
Hua, Rui
Zhang, Xinxin
Yan, Ting Yu
Tong, Yang
Zhao, Xin
Chen, Shi Chao
Wang, Moying
Bressler, Neil M.
Kong, Jun
Scleral Cross-Linking in Form-Deprivation Myopic Guinea Pig Eyes Leads to Glaucomatous Changes
title Scleral Cross-Linking in Form-Deprivation Myopic Guinea Pig Eyes Leads to Glaucomatous Changes
title_full Scleral Cross-Linking in Form-Deprivation Myopic Guinea Pig Eyes Leads to Glaucomatous Changes
title_fullStr Scleral Cross-Linking in Form-Deprivation Myopic Guinea Pig Eyes Leads to Glaucomatous Changes
title_full_unstemmed Scleral Cross-Linking in Form-Deprivation Myopic Guinea Pig Eyes Leads to Glaucomatous Changes
title_short Scleral Cross-Linking in Form-Deprivation Myopic Guinea Pig Eyes Leads to Glaucomatous Changes
title_sort scleral cross-linking in form-deprivation myopic guinea pig eyes leads to glaucomatous changes
topic Anatomy and Pathology/Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150827/
https://www.ncbi.nlm.nih.gov/pubmed/35594036
http://dx.doi.org/10.1167/iovs.63.5.24
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