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Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis

Although ubiquitously present, information on the function of complex N-glycan posttranslational modification in plants is very limited and is often neglected. In this work, we adopted an enzyme-assisted matrix-assisted laser desorption/ionization mass spectrometry imaging strategy to visualize the...

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Autores principales: Veličković, Dušan, Liao, Yen-Chen, Thibert, Stephanie, Veličković, Marija, Anderton, Christopher, Voglmeir, Josef, Stacey, Gary, Zhou, Mowei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150855/
https://www.ncbi.nlm.nih.gov/pubmed/35651768
http://dx.doi.org/10.3389/fpls.2022.869281
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author Veličković, Dušan
Liao, Yen-Chen
Thibert, Stephanie
Veličković, Marija
Anderton, Christopher
Voglmeir, Josef
Stacey, Gary
Zhou, Mowei
author_facet Veličković, Dušan
Liao, Yen-Chen
Thibert, Stephanie
Veličković, Marija
Anderton, Christopher
Voglmeir, Josef
Stacey, Gary
Zhou, Mowei
author_sort Veličković, Dušan
collection PubMed
description Although ubiquitously present, information on the function of complex N-glycan posttranslational modification in plants is very limited and is often neglected. In this work, we adopted an enzyme-assisted matrix-assisted laser desorption/ionization mass spectrometry imaging strategy to visualize the distribution and identity of N-glycans in soybean root nodules at a cellular resolution. We additionally performed proteomics analysis to probe the potential correlation to proteome changes during symbiotic rhizobia-legume interactions. Our ion images reveal that intense N-glycosylation occurs in the sclerenchyma layer, and inside the infected cells within the infection zone, while morphological structures such as the cortex, uninfected cells, and cells that form the attachment with the root are fewer N-glycosylated. Notably, we observed different N-glycan profiles between soybean root nodules infected with wild-type rhizobia and those infected with mutant rhizobia incapable of efficiently fixing atmospheric nitrogen. The majority of complex N-glycan structures, particularly those with characteristic Lewis-a epitopes, are more abundant in the mutant nodules. Our proteomic results revealed that these glycans likely originated from proteins that maintain the redox balance crucial for proper nitrogen fixation, but also from enzymes involved in N-glycan and phenylpropanoid biosynthesis. These findings indicate the possible involvement of Lewis-a glycans in these critical pathways during legume-rhizobia symbiosis.
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spelling pubmed-91508552022-05-31 Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis Veličković, Dušan Liao, Yen-Chen Thibert, Stephanie Veličković, Marija Anderton, Christopher Voglmeir, Josef Stacey, Gary Zhou, Mowei Front Plant Sci Plant Science Although ubiquitously present, information on the function of complex N-glycan posttranslational modification in plants is very limited and is often neglected. In this work, we adopted an enzyme-assisted matrix-assisted laser desorption/ionization mass spectrometry imaging strategy to visualize the distribution and identity of N-glycans in soybean root nodules at a cellular resolution. We additionally performed proteomics analysis to probe the potential correlation to proteome changes during symbiotic rhizobia-legume interactions. Our ion images reveal that intense N-glycosylation occurs in the sclerenchyma layer, and inside the infected cells within the infection zone, while morphological structures such as the cortex, uninfected cells, and cells that form the attachment with the root are fewer N-glycosylated. Notably, we observed different N-glycan profiles between soybean root nodules infected with wild-type rhizobia and those infected with mutant rhizobia incapable of efficiently fixing atmospheric nitrogen. The majority of complex N-glycan structures, particularly those with characteristic Lewis-a epitopes, are more abundant in the mutant nodules. Our proteomic results revealed that these glycans likely originated from proteins that maintain the redox balance crucial for proper nitrogen fixation, but also from enzymes involved in N-glycan and phenylpropanoid biosynthesis. These findings indicate the possible involvement of Lewis-a glycans in these critical pathways during legume-rhizobia symbiosis. Frontiers Media S.A. 2022-05-16 /pmc/articles/PMC9150855/ /pubmed/35651768 http://dx.doi.org/10.3389/fpls.2022.869281 Text en Copyright © 2022 Veličković, Liao, Thibert, Veličković, Anderton, Voglmeir, Stacey and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Veličković, Dušan
Liao, Yen-Chen
Thibert, Stephanie
Veličković, Marija
Anderton, Christopher
Voglmeir, Josef
Stacey, Gary
Zhou, Mowei
Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis
title Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis
title_full Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis
title_fullStr Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis
title_full_unstemmed Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis
title_short Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis
title_sort spatial mapping of plant n-glycosylation cellular heterogeneity inside soybean root nodules provided insights into legume-rhizobia symbiosis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150855/
https://www.ncbi.nlm.nih.gov/pubmed/35651768
http://dx.doi.org/10.3389/fpls.2022.869281
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