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Synthetic spatial patterning in bacteria: advances based on novel diffusible signals
Engineering multicellular patterning may help in the understanding of some fundamental laws of pattern formation and thus may contribute to the field of developmental biology. Furthermore, advanced spatial control over gene expression may revolutionize fields such as medicine, through organoid or ti...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9151330/ https://www.ncbi.nlm.nih.gov/pubmed/34843638 http://dx.doi.org/10.1111/1751-7915.13979 |
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author | Oliver Huidobro, Martina Tica, Jure Wachter, Georg K. A. Isalan, Mark |
author_facet | Oliver Huidobro, Martina Tica, Jure Wachter, Georg K. A. Isalan, Mark |
author_sort | Oliver Huidobro, Martina |
collection | PubMed |
description | Engineering multicellular patterning may help in the understanding of some fundamental laws of pattern formation and thus may contribute to the field of developmental biology. Furthermore, advanced spatial control over gene expression may revolutionize fields such as medicine, through organoid or tissue engineering. To date, foundational advances in spatial synthetic biology have often been made in prokaryotes, using artificial gene circuits. In this review, engineered patterns are classified into four levels of increasing complexity, ranging from spatial systems with no diffusible signals to systems with complex multi‐diffusor interactions. This classification highlights how the field was held back by a lack of diffusible components. Consequently, we provide a summary of both previously characterized and some new potential candidate small‐molecule signals that can regulate gene expression in Escherichia coli. These diffusive signals will help synthetic biologists to successfully engineer increasingly intricate, robust and tuneable spatial structures. |
format | Online Article Text |
id | pubmed-9151330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91513302022-06-04 Synthetic spatial patterning in bacteria: advances based on novel diffusible signals Oliver Huidobro, Martina Tica, Jure Wachter, Georg K. A. Isalan, Mark Microb Biotechnol Opinion Engineering multicellular patterning may help in the understanding of some fundamental laws of pattern formation and thus may contribute to the field of developmental biology. Furthermore, advanced spatial control over gene expression may revolutionize fields such as medicine, through organoid or tissue engineering. To date, foundational advances in spatial synthetic biology have often been made in prokaryotes, using artificial gene circuits. In this review, engineered patterns are classified into four levels of increasing complexity, ranging from spatial systems with no diffusible signals to systems with complex multi‐diffusor interactions. This classification highlights how the field was held back by a lack of diffusible components. Consequently, we provide a summary of both previously characterized and some new potential candidate small‐molecule signals that can regulate gene expression in Escherichia coli. These diffusive signals will help synthetic biologists to successfully engineer increasingly intricate, robust and tuneable spatial structures. John Wiley and Sons Inc. 2021-11-29 /pmc/articles/PMC9151330/ /pubmed/34843638 http://dx.doi.org/10.1111/1751-7915.13979 Text en © 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Opinion Oliver Huidobro, Martina Tica, Jure Wachter, Georg K. A. Isalan, Mark Synthetic spatial patterning in bacteria: advances based on novel diffusible signals |
title | Synthetic spatial patterning in bacteria: advances based on novel diffusible signals |
title_full | Synthetic spatial patterning in bacteria: advances based on novel diffusible signals |
title_fullStr | Synthetic spatial patterning in bacteria: advances based on novel diffusible signals |
title_full_unstemmed | Synthetic spatial patterning in bacteria: advances based on novel diffusible signals |
title_short | Synthetic spatial patterning in bacteria: advances based on novel diffusible signals |
title_sort | synthetic spatial patterning in bacteria: advances based on novel diffusible signals |
topic | Opinion |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9151330/ https://www.ncbi.nlm.nih.gov/pubmed/34843638 http://dx.doi.org/10.1111/1751-7915.13979 |
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