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Synthetic spatial patterning in bacteria: advances based on novel diffusible signals

Engineering multicellular patterning may help in the understanding of some fundamental laws of pattern formation and thus may contribute to the field of developmental biology. Furthermore, advanced spatial control over gene expression may revolutionize fields such as medicine, through organoid or ti...

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Autores principales: Oliver Huidobro, Martina, Tica, Jure, Wachter, Georg K. A., Isalan, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9151330/
https://www.ncbi.nlm.nih.gov/pubmed/34843638
http://dx.doi.org/10.1111/1751-7915.13979
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author Oliver Huidobro, Martina
Tica, Jure
Wachter, Georg K. A.
Isalan, Mark
author_facet Oliver Huidobro, Martina
Tica, Jure
Wachter, Georg K. A.
Isalan, Mark
author_sort Oliver Huidobro, Martina
collection PubMed
description Engineering multicellular patterning may help in the understanding of some fundamental laws of pattern formation and thus may contribute to the field of developmental biology. Furthermore, advanced spatial control over gene expression may revolutionize fields such as medicine, through organoid or tissue engineering. To date, foundational advances in spatial synthetic biology have often been made in prokaryotes, using artificial gene circuits. In this review, engineered patterns are classified into four levels of increasing complexity, ranging from spatial systems with no diffusible signals to systems with complex multi‐diffusor interactions. This classification highlights how the field was held back by a lack of diffusible components. Consequently, we provide a summary of both previously characterized and some new potential candidate small‐molecule signals that can regulate gene expression in Escherichia coli. These diffusive signals will help synthetic biologists to successfully engineer increasingly intricate, robust and tuneable spatial structures.
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spelling pubmed-91513302022-06-04 Synthetic spatial patterning in bacteria: advances based on novel diffusible signals Oliver Huidobro, Martina Tica, Jure Wachter, Georg K. A. Isalan, Mark Microb Biotechnol Opinion Engineering multicellular patterning may help in the understanding of some fundamental laws of pattern formation and thus may contribute to the field of developmental biology. Furthermore, advanced spatial control over gene expression may revolutionize fields such as medicine, through organoid or tissue engineering. To date, foundational advances in spatial synthetic biology have often been made in prokaryotes, using artificial gene circuits. In this review, engineered patterns are classified into four levels of increasing complexity, ranging from spatial systems with no diffusible signals to systems with complex multi‐diffusor interactions. This classification highlights how the field was held back by a lack of diffusible components. Consequently, we provide a summary of both previously characterized and some new potential candidate small‐molecule signals that can regulate gene expression in Escherichia coli. These diffusive signals will help synthetic biologists to successfully engineer increasingly intricate, robust and tuneable spatial structures. John Wiley and Sons Inc. 2021-11-29 /pmc/articles/PMC9151330/ /pubmed/34843638 http://dx.doi.org/10.1111/1751-7915.13979 Text en © 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Opinion
Oliver Huidobro, Martina
Tica, Jure
Wachter, Georg K. A.
Isalan, Mark
Synthetic spatial patterning in bacteria: advances based on novel diffusible signals
title Synthetic spatial patterning in bacteria: advances based on novel diffusible signals
title_full Synthetic spatial patterning in bacteria: advances based on novel diffusible signals
title_fullStr Synthetic spatial patterning in bacteria: advances based on novel diffusible signals
title_full_unstemmed Synthetic spatial patterning in bacteria: advances based on novel diffusible signals
title_short Synthetic spatial patterning in bacteria: advances based on novel diffusible signals
title_sort synthetic spatial patterning in bacteria: advances based on novel diffusible signals
topic Opinion
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9151330/
https://www.ncbi.nlm.nih.gov/pubmed/34843638
http://dx.doi.org/10.1111/1751-7915.13979
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