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Developing an understanding of sophorolipid synthesis through application of a central composite design model

A key barrier to market penetration for sophorolipid biosurfactants is the ability to improve productivity and utilize alternative feedstocks to reduce the cost of production. To do this, a suitable screening tool is required that is able to model the interactions between media components and alter...

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Detalles Bibliográficos
Autores principales: Ingham, Benjamin, Winterburn, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9151336/
https://www.ncbi.nlm.nih.gov/pubmed/35038384
http://dx.doi.org/10.1111/1751-7915.14003
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author Ingham, Benjamin
Winterburn, James
author_facet Ingham, Benjamin
Winterburn, James
author_sort Ingham, Benjamin
collection PubMed
description A key barrier to market penetration for sophorolipid biosurfactants is the ability to improve productivity and utilize alternative feedstocks to reduce the cost of production. To do this, a suitable screening tool is required that is able to model the interactions between media components and alter conditions to maximize productivity. In the following work, a central composite design is applied to analyse the effects of altering glucose, rapeseed oil, corn steep liquor and ammonium sulphate concentrations on sophorolipid production with Starmerella bombicola ATCC 222144 after 168 h. Sophorolipid production was analysed using standard least squares regression and the findings related to the growth (OD(600)) and broth conditions (glucose, glycerol and oil concentration). An optimum media composition was found that was capable of producing 39.5 g l(–1) sophorolipid. Nitrogen and rapeseed oil sources were found to be significant, linked to their role in growth and substrate supply respectively. Glucose did not demonstrate a significant effect on production despite its importance to biosynthesis and its depletion in the broth within 96 h, instead being replaced by glycerol (via triglyceride breakdown) as the hydrophilic carbon source at the point of glucose depletion. A large dataset was obtained, and a regression model with applications towards substrate screening and process optimisation developed.
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spelling pubmed-91513362022-06-04 Developing an understanding of sophorolipid synthesis through application of a central composite design model Ingham, Benjamin Winterburn, James Microb Biotechnol Minireviews A key barrier to market penetration for sophorolipid biosurfactants is the ability to improve productivity and utilize alternative feedstocks to reduce the cost of production. To do this, a suitable screening tool is required that is able to model the interactions between media components and alter conditions to maximize productivity. In the following work, a central composite design is applied to analyse the effects of altering glucose, rapeseed oil, corn steep liquor and ammonium sulphate concentrations on sophorolipid production with Starmerella bombicola ATCC 222144 after 168 h. Sophorolipid production was analysed using standard least squares regression and the findings related to the growth (OD(600)) and broth conditions (glucose, glycerol and oil concentration). An optimum media composition was found that was capable of producing 39.5 g l(–1) sophorolipid. Nitrogen and rapeseed oil sources were found to be significant, linked to their role in growth and substrate supply respectively. Glucose did not demonstrate a significant effect on production despite its importance to biosynthesis and its depletion in the broth within 96 h, instead being replaced by glycerol (via triglyceride breakdown) as the hydrophilic carbon source at the point of glucose depletion. A large dataset was obtained, and a regression model with applications towards substrate screening and process optimisation developed. John Wiley and Sons Inc. 2022-01-17 /pmc/articles/PMC9151336/ /pubmed/35038384 http://dx.doi.org/10.1111/1751-7915.14003 Text en © 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Minireviews
Ingham, Benjamin
Winterburn, James
Developing an understanding of sophorolipid synthesis through application of a central composite design model
title Developing an understanding of sophorolipid synthesis through application of a central composite design model
title_full Developing an understanding of sophorolipid synthesis through application of a central composite design model
title_fullStr Developing an understanding of sophorolipid synthesis through application of a central composite design model
title_full_unstemmed Developing an understanding of sophorolipid synthesis through application of a central composite design model
title_short Developing an understanding of sophorolipid synthesis through application of a central composite design model
title_sort developing an understanding of sophorolipid synthesis through application of a central composite design model
topic Minireviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9151336/
https://www.ncbi.nlm.nih.gov/pubmed/35038384
http://dx.doi.org/10.1111/1751-7915.14003
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