Cargando…

Dose Variations Using an X-Ray Cabinet to Establish in vitro Dose-Response Curves for Biological Dosimetry Assays

In biological dosimetry, dose-response curves are essential for reliable retrospective dose estimation of individual exposure in case of a radiation accident. Therefore, blood samples are irradiated in vitro and evaluated based on the applied assay. Accurate physical dosimetry of the irradiation per...

Descripción completa

Detalles Bibliográficos
Autores principales: Bucher, Martin, Weiss, Tina, Endesfelder, David, Trompier, Francois, Ristic, Yoann, Kunert, Patrizia, Schlattl, Helmut, Giussani, Augusto, Oestreicher, Ursula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9152255/
https://www.ncbi.nlm.nih.gov/pubmed/35655448
http://dx.doi.org/10.3389/fpubh.2022.903509
_version_ 1784717606454820864
author Bucher, Martin
Weiss, Tina
Endesfelder, David
Trompier, Francois
Ristic, Yoann
Kunert, Patrizia
Schlattl, Helmut
Giussani, Augusto
Oestreicher, Ursula
author_facet Bucher, Martin
Weiss, Tina
Endesfelder, David
Trompier, Francois
Ristic, Yoann
Kunert, Patrizia
Schlattl, Helmut
Giussani, Augusto
Oestreicher, Ursula
author_sort Bucher, Martin
collection PubMed
description In biological dosimetry, dose-response curves are essential for reliable retrospective dose estimation of individual exposure in case of a radiation accident. Therefore, blood samples are irradiated in vitro and evaluated based on the applied assay. Accurate physical dosimetry of the irradiation performance is a critical part of the experimental procedure and is influenced by the experimental setup, especially when X-ray cabinets are used. The aim of this study was to investigate variations and pitfalls associated with the experimental setups used to establish calibration curves in biological dosimetry with X-ray cabinets. In this study, irradiation was performed with an X-ray source (195 kV, 10 mA, 0.5 mm Cu filter, dose rate 0.52 Gy/min, 1(st) and 2(nd) half-value layer = 1.01 and 1.76 mm Cu, respectively, average energy 86.9 keV). Blood collection tubes were irradiated with a dose of 1 Gy in vertical or horizontal orientation in the center of the beam area with or without usage of an additional fan heater. To evaluate the influence of the setups, physical dose measurements using thermoluminescence dosimeters, electron paramagnetic resonance dosimetry and ionization chamber as well as biological effects, quantified by dicentric chromosomes and micronuclei, were compared. This study revealed that the orientation of the sample tubes (vertical vs. horizontal) had a significant effect on the radiation dose with a variation of −41% up to +49% and contributed to a dose gradient of up to 870 mGy inside the vertical tubes due to the size of the sample tubes and the associated differences in the distance to the focal point of the tube. The number of dicentric chromosomes and micronuclei differed by ~30% between both orientations. An additional fan heater had no consistent impact. Therefore, dosimetric monitoring of experimental irradiation setups is mandatory prior to the establishment of calibration curves in biological dosimetry. Careful consideration of the experimental setup in collaboration with physicists is required to ensure traceability and reproducibility of irradiation conditions, to correlate the radiation dose and the number of aberrations correctly and to avoid systematical bias influencing the dose estimation in the frame of biological dosimetry.
format Online
Article
Text
id pubmed-9152255
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91522552022-06-01 Dose Variations Using an X-Ray Cabinet to Establish in vitro Dose-Response Curves for Biological Dosimetry Assays Bucher, Martin Weiss, Tina Endesfelder, David Trompier, Francois Ristic, Yoann Kunert, Patrizia Schlattl, Helmut Giussani, Augusto Oestreicher, Ursula Front Public Health Public Health In biological dosimetry, dose-response curves are essential for reliable retrospective dose estimation of individual exposure in case of a radiation accident. Therefore, blood samples are irradiated in vitro and evaluated based on the applied assay. Accurate physical dosimetry of the irradiation performance is a critical part of the experimental procedure and is influenced by the experimental setup, especially when X-ray cabinets are used. The aim of this study was to investigate variations and pitfalls associated with the experimental setups used to establish calibration curves in biological dosimetry with X-ray cabinets. In this study, irradiation was performed with an X-ray source (195 kV, 10 mA, 0.5 mm Cu filter, dose rate 0.52 Gy/min, 1(st) and 2(nd) half-value layer = 1.01 and 1.76 mm Cu, respectively, average energy 86.9 keV). Blood collection tubes were irradiated with a dose of 1 Gy in vertical or horizontal orientation in the center of the beam area with or without usage of an additional fan heater. To evaluate the influence of the setups, physical dose measurements using thermoluminescence dosimeters, electron paramagnetic resonance dosimetry and ionization chamber as well as biological effects, quantified by dicentric chromosomes and micronuclei, were compared. This study revealed that the orientation of the sample tubes (vertical vs. horizontal) had a significant effect on the radiation dose with a variation of −41% up to +49% and contributed to a dose gradient of up to 870 mGy inside the vertical tubes due to the size of the sample tubes and the associated differences in the distance to the focal point of the tube. The number of dicentric chromosomes and micronuclei differed by ~30% between both orientations. An additional fan heater had no consistent impact. Therefore, dosimetric monitoring of experimental irradiation setups is mandatory prior to the establishment of calibration curves in biological dosimetry. Careful consideration of the experimental setup in collaboration with physicists is required to ensure traceability and reproducibility of irradiation conditions, to correlate the radiation dose and the number of aberrations correctly and to avoid systematical bias influencing the dose estimation in the frame of biological dosimetry. Frontiers Media S.A. 2022-05-17 /pmc/articles/PMC9152255/ /pubmed/35655448 http://dx.doi.org/10.3389/fpubh.2022.903509 Text en Copyright © 2022 Bucher, Weiss, Endesfelder, Trompier, Ristic, Kunert, Schlattl, Giussani and Oestreicher. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Bucher, Martin
Weiss, Tina
Endesfelder, David
Trompier, Francois
Ristic, Yoann
Kunert, Patrizia
Schlattl, Helmut
Giussani, Augusto
Oestreicher, Ursula
Dose Variations Using an X-Ray Cabinet to Establish in vitro Dose-Response Curves for Biological Dosimetry Assays
title Dose Variations Using an X-Ray Cabinet to Establish in vitro Dose-Response Curves for Biological Dosimetry Assays
title_full Dose Variations Using an X-Ray Cabinet to Establish in vitro Dose-Response Curves for Biological Dosimetry Assays
title_fullStr Dose Variations Using an X-Ray Cabinet to Establish in vitro Dose-Response Curves for Biological Dosimetry Assays
title_full_unstemmed Dose Variations Using an X-Ray Cabinet to Establish in vitro Dose-Response Curves for Biological Dosimetry Assays
title_short Dose Variations Using an X-Ray Cabinet to Establish in vitro Dose-Response Curves for Biological Dosimetry Assays
title_sort dose variations using an x-ray cabinet to establish in vitro dose-response curves for biological dosimetry assays
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9152255/
https://www.ncbi.nlm.nih.gov/pubmed/35655448
http://dx.doi.org/10.3389/fpubh.2022.903509
work_keys_str_mv AT buchermartin dosevariationsusinganxraycabinettoestablishinvitrodoseresponsecurvesforbiologicaldosimetryassays
AT weisstina dosevariationsusinganxraycabinettoestablishinvitrodoseresponsecurvesforbiologicaldosimetryassays
AT endesfelderdavid dosevariationsusinganxraycabinettoestablishinvitrodoseresponsecurvesforbiologicaldosimetryassays
AT trompierfrancois dosevariationsusinganxraycabinettoestablishinvitrodoseresponsecurvesforbiologicaldosimetryassays
AT risticyoann dosevariationsusinganxraycabinettoestablishinvitrodoseresponsecurvesforbiologicaldosimetryassays
AT kunertpatrizia dosevariationsusinganxraycabinettoestablishinvitrodoseresponsecurvesforbiologicaldosimetryassays
AT schlattlhelmut dosevariationsusinganxraycabinettoestablishinvitrodoseresponsecurvesforbiologicaldosimetryassays
AT giussaniaugusto dosevariationsusinganxraycabinettoestablishinvitrodoseresponsecurvesforbiologicaldosimetryassays
AT oestreicherursula dosevariationsusinganxraycabinettoestablishinvitrodoseresponsecurvesforbiologicaldosimetryassays