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Diagnostic accuracy of fluorescence flow-cytometry technology using Sysmex XN-31 for imported malaria in a non-endemic setting
Malaria diagnosis based on microscopy is impaired by the gradual disappearance of experienced microscopists in non-endemic areas. Aside from the conventional diagnostic methods, fluorescence flow cytometry technology using Sysmex XN-31, an automated haematology analyser, has been registered to suppo...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
EDP Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9153516/ https://www.ncbi.nlm.nih.gov/pubmed/35638753 http://dx.doi.org/10.1051/parasite/2022031 |
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author | Picot, Stéphane Perpoint, Thomas Chidiac, Christian Sigal, Alain Javouhey, Etienne Gillet, Yves Jacquin, Laurent Douplat, Marion Tazarourte, Karim Argaud, Laurent Wallon, Martine Miossec, Charline Bonnot, Guillaume Bienvenu, Anne-Lise |
author_facet | Picot, Stéphane Perpoint, Thomas Chidiac, Christian Sigal, Alain Javouhey, Etienne Gillet, Yves Jacquin, Laurent Douplat, Marion Tazarourte, Karim Argaud, Laurent Wallon, Martine Miossec, Charline Bonnot, Guillaume Bienvenu, Anne-Lise |
author_sort | Picot, Stéphane |
collection | PubMed |
description | Malaria diagnosis based on microscopy is impaired by the gradual disappearance of experienced microscopists in non-endemic areas. Aside from the conventional diagnostic methods, fluorescence flow cytometry technology using Sysmex XN-31, an automated haematology analyser, has been registered to support malaria diagnosis. The aim of this prospective, monocentric, non-interventional study was to evaluate the diagnostic accuracy of the XN-31 for the initial diagnosis or follow-up of imported malaria cases compared to the reference malaria tests including microscopy, loop mediated isothermal amplification, and rapid diagnostic tests. Over a one-year period, 357 blood samples were analysed, including 248 negative and 109 positive malaria samples. Compared to microscopy, XN-31 showed sensitivity of 100% (95% CI: 97.13–100) and specificity of 98.39% (95% CI: 95.56–100) for the initial diagnosis of imported malaria cases. Moreover, it provided accurate species identification asfalciparumor non-falciparumand parasitaemia determination in a very short time compared to other methods. We also demonstrated that XN-31 was a reliable method for patient follow-up on days 3, 7, and 28. Malaria diagnosis can be improved in non-endemic areas by the use of dedicated haematology analysers coupled with standard microscopy or other methods in development, such as artificial intelligence for blood slide reading. Given that XN-31 provided an accurate diagnosis in 1 min, it may reduce the time interval before treatment and thus improve the outcome of patient who have malaria. |
format | Online Article Text |
id | pubmed-9153516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-91535162022-06-13 Diagnostic accuracy of fluorescence flow-cytometry technology using Sysmex XN-31 for imported malaria in a non-endemic setting Picot, Stéphane Perpoint, Thomas Chidiac, Christian Sigal, Alain Javouhey, Etienne Gillet, Yves Jacquin, Laurent Douplat, Marion Tazarourte, Karim Argaud, Laurent Wallon, Martine Miossec, Charline Bonnot, Guillaume Bienvenu, Anne-Lise Parasite Research Article Malaria diagnosis based on microscopy is impaired by the gradual disappearance of experienced microscopists in non-endemic areas. Aside from the conventional diagnostic methods, fluorescence flow cytometry technology using Sysmex XN-31, an automated haematology analyser, has been registered to support malaria diagnosis. The aim of this prospective, monocentric, non-interventional study was to evaluate the diagnostic accuracy of the XN-31 for the initial diagnosis or follow-up of imported malaria cases compared to the reference malaria tests including microscopy, loop mediated isothermal amplification, and rapid diagnostic tests. Over a one-year period, 357 blood samples were analysed, including 248 negative and 109 positive malaria samples. Compared to microscopy, XN-31 showed sensitivity of 100% (95% CI: 97.13–100) and specificity of 98.39% (95% CI: 95.56–100) for the initial diagnosis of imported malaria cases. Moreover, it provided accurate species identification asfalciparumor non-falciparumand parasitaemia determination in a very short time compared to other methods. We also demonstrated that XN-31 was a reliable method for patient follow-up on days 3, 7, and 28. Malaria diagnosis can be improved in non-endemic areas by the use of dedicated haematology analysers coupled with standard microscopy or other methods in development, such as artificial intelligence for blood slide reading. Given that XN-31 provided an accurate diagnosis in 1 min, it may reduce the time interval before treatment and thus improve the outcome of patient who have malaria. EDP Sciences 2022-05-31 /pmc/articles/PMC9153516/ /pubmed/35638753 http://dx.doi.org/10.1051/parasite/2022031 Text en © S. Picot et al., published by EDP Sciences, 2022 https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Picot, Stéphane Perpoint, Thomas Chidiac, Christian Sigal, Alain Javouhey, Etienne Gillet, Yves Jacquin, Laurent Douplat, Marion Tazarourte, Karim Argaud, Laurent Wallon, Martine Miossec, Charline Bonnot, Guillaume Bienvenu, Anne-Lise Diagnostic accuracy of fluorescence flow-cytometry technology using Sysmex XN-31 for imported malaria in a non-endemic setting |
title | Diagnostic accuracy of fluorescence flow-cytometry technology using Sysmex XN-31 for imported malaria in a non-endemic setting |
title_full | Diagnostic accuracy of fluorescence flow-cytometry technology using Sysmex XN-31 for imported malaria in a non-endemic setting |
title_fullStr | Diagnostic accuracy of fluorescence flow-cytometry technology using Sysmex XN-31 for imported malaria in a non-endemic setting |
title_full_unstemmed | Diagnostic accuracy of fluorescence flow-cytometry technology using Sysmex XN-31 for imported malaria in a non-endemic setting |
title_short | Diagnostic accuracy of fluorescence flow-cytometry technology using Sysmex XN-31 for imported malaria in a non-endemic setting |
title_sort | diagnostic accuracy of fluorescence flow-cytometry technology using sysmex xn-31 for imported malaria in a non-endemic setting |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9153516/ https://www.ncbi.nlm.nih.gov/pubmed/35638753 http://dx.doi.org/10.1051/parasite/2022031 |
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