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Flow cytometry can reliably capture gut microbial composition in healthy adults as well as dysbiosis dynamics in patients with aggressive B-cell non-Hodgkin lymphoma

Modulation of commensal gut microbiota is increasingly recognized as a promising strategy to reduce mortality in patients with malignant diseases, but monitoring for dysbiosis is generally not routine clinical practice due to equipment, expertise and funding required for sequencing analysis. A low-t...

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Autores principales: Schmiester, Maren, Maier, René, Riedel, René, Durek, Pawel, Frentsch, Marco, Kolling, Stefan, Mashreghi, Mir-Farzin, Jenq, Robert, Zhang, Liangliang, Peterson, Christine B., Bullinger, Lars, Chang, Hyun-Dong, Na, Il-Kang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9154785/
https://www.ncbi.nlm.nih.gov/pubmed/35634713
http://dx.doi.org/10.1080/19490976.2022.2081475
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author Schmiester, Maren
Maier, René
Riedel, René
Durek, Pawel
Frentsch, Marco
Kolling, Stefan
Mashreghi, Mir-Farzin
Jenq, Robert
Zhang, Liangliang
Peterson, Christine B.
Bullinger, Lars
Chang, Hyun-Dong
Na, Il-Kang
author_facet Schmiester, Maren
Maier, René
Riedel, René
Durek, Pawel
Frentsch, Marco
Kolling, Stefan
Mashreghi, Mir-Farzin
Jenq, Robert
Zhang, Liangliang
Peterson, Christine B.
Bullinger, Lars
Chang, Hyun-Dong
Na, Il-Kang
author_sort Schmiester, Maren
collection PubMed
description Modulation of commensal gut microbiota is increasingly recognized as a promising strategy to reduce mortality in patients with malignant diseases, but monitoring for dysbiosis is generally not routine clinical practice due to equipment, expertise and funding required for sequencing analysis. A low-threshold alternative is microbial diversity profiling by single-cell flow cytometry (FCM), which we compared to 16S rRNA sequencing in human fecal samples and employed to characterize longitudinal changes in the microbiome composition of patients with aggressive B-cell non-Hodgkin lymphoma undergoing chemoimmunotherapy. Diversity measures obtained from both methods were correlated and captured identical trends in microbial community structures, finding no difference in patients’ pretreatment alpha or beta diversity compared to healthy controls and a significant and progressive loss of alpha diversity during chemoimmunotherapy. Our results highlight the potential of FCM-based microbiome profiling as a reliable and accessible diagnostic tool that can provide novel insights into cancer therapy-associated dysbiosis dynamics.
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spelling pubmed-91547852022-06-01 Flow cytometry can reliably capture gut microbial composition in healthy adults as well as dysbiosis dynamics in patients with aggressive B-cell non-Hodgkin lymphoma Schmiester, Maren Maier, René Riedel, René Durek, Pawel Frentsch, Marco Kolling, Stefan Mashreghi, Mir-Farzin Jenq, Robert Zhang, Liangliang Peterson, Christine B. Bullinger, Lars Chang, Hyun-Dong Na, Il-Kang Gut Microbes Brief Report Modulation of commensal gut microbiota is increasingly recognized as a promising strategy to reduce mortality in patients with malignant diseases, but monitoring for dysbiosis is generally not routine clinical practice due to equipment, expertise and funding required for sequencing analysis. A low-threshold alternative is microbial diversity profiling by single-cell flow cytometry (FCM), which we compared to 16S rRNA sequencing in human fecal samples and employed to characterize longitudinal changes in the microbiome composition of patients with aggressive B-cell non-Hodgkin lymphoma undergoing chemoimmunotherapy. Diversity measures obtained from both methods were correlated and captured identical trends in microbial community structures, finding no difference in patients’ pretreatment alpha or beta diversity compared to healthy controls and a significant and progressive loss of alpha diversity during chemoimmunotherapy. Our results highlight the potential of FCM-based microbiome profiling as a reliable and accessible diagnostic tool that can provide novel insights into cancer therapy-associated dysbiosis dynamics. Taylor & Francis 2022-05-29 /pmc/articles/PMC9154785/ /pubmed/35634713 http://dx.doi.org/10.1080/19490976.2022.2081475 Text en © 2022 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Report
Schmiester, Maren
Maier, René
Riedel, René
Durek, Pawel
Frentsch, Marco
Kolling, Stefan
Mashreghi, Mir-Farzin
Jenq, Robert
Zhang, Liangliang
Peterson, Christine B.
Bullinger, Lars
Chang, Hyun-Dong
Na, Il-Kang
Flow cytometry can reliably capture gut microbial composition in healthy adults as well as dysbiosis dynamics in patients with aggressive B-cell non-Hodgkin lymphoma
title Flow cytometry can reliably capture gut microbial composition in healthy adults as well as dysbiosis dynamics in patients with aggressive B-cell non-Hodgkin lymphoma
title_full Flow cytometry can reliably capture gut microbial composition in healthy adults as well as dysbiosis dynamics in patients with aggressive B-cell non-Hodgkin lymphoma
title_fullStr Flow cytometry can reliably capture gut microbial composition in healthy adults as well as dysbiosis dynamics in patients with aggressive B-cell non-Hodgkin lymphoma
title_full_unstemmed Flow cytometry can reliably capture gut microbial composition in healthy adults as well as dysbiosis dynamics in patients with aggressive B-cell non-Hodgkin lymphoma
title_short Flow cytometry can reliably capture gut microbial composition in healthy adults as well as dysbiosis dynamics in patients with aggressive B-cell non-Hodgkin lymphoma
title_sort flow cytometry can reliably capture gut microbial composition in healthy adults as well as dysbiosis dynamics in patients with aggressive b-cell non-hodgkin lymphoma
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9154785/
https://www.ncbi.nlm.nih.gov/pubmed/35634713
http://dx.doi.org/10.1080/19490976.2022.2081475
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