Cargando…
Analysis of selected polymorphisms in FOXP3 gene in a cohort of Egyptian patients with schizophrenia
BACKGROUND: Schizophrenia is a chronic mental disorder with different symptoms. The environmental and genetic factors are suggested to be the etiology of schizophrenia. However, the exact cause and pathogenesis of schizophrenia are still unclear. Different studies suggested that the immune system ma...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9156649/ https://www.ncbi.nlm.nih.gov/pubmed/35641708 http://dx.doi.org/10.1186/s43141-022-00371-y |
_version_ | 1784718481390829568 |
---|---|
author | Mostafa, Maged Fathy, Aya Ahmed Elwasify, Mohamed Abdelsalam, Maha |
author_facet | Mostafa, Maged Fathy, Aya Ahmed Elwasify, Mohamed Abdelsalam, Maha |
author_sort | Mostafa, Maged |
collection | PubMed |
description | BACKGROUND: Schizophrenia is a chronic mental disorder with different symptoms. The environmental and genetic factors are suggested to be the etiology of schizophrenia. However, the exact cause and pathogenesis of schizophrenia are still unclear. Different studies suggested that the immune system may have a role in schizophrenia. A genetic study found a relation between the disease and the HLA region on the sixth chromosome. Regulatory T cells (Treg) have a role in the regulation of immune response, especially the balance between TH1 and TH2 cells. The FOXP3 protein is a key regulator for Treg cell’s functions. FOXP3 is a transcriptional factor, and its gene is present on the short arm of the X chromosome. The selected SNPs present in the promoter region which act as binding sites for transcriptional factors. This study investigated FOXP3 gene polymorphisms (rs3761548, rs3761549, and rs2232365) in Egyptian patients with schizophrenia. There are no previous studies about the association of FOXP3 gene polymorphisms with schizophrenia. The three selected single-nucleotide polymorphisms (SNPs) were investigated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for 125 schizophrenia patients and 160 healthy controls. The Positive and Negative Syndrome Scale (PANSS) was used to evaluate patients with schizophrenia. RESULTS: No significant associations were found between schizophrenia patients and healthy controls for the alleles and genotypes of the selected SNPs (P-value > 0.05). However, a significant association with ACC and ATC haplotypes was detected (P-value 0.001). No significant association was detected between the PANSS score and any of the studied SNPs. CONCLUSION: The ATC haplotype of rs2232365, rs3761549, and rs3761548 could be considered a risk factor for schizophrenia in Egyptian patients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43141-022-00371-y. |
format | Online Article Text |
id | pubmed-9156649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-91566492022-06-16 Analysis of selected polymorphisms in FOXP3 gene in a cohort of Egyptian patients with schizophrenia Mostafa, Maged Fathy, Aya Ahmed Elwasify, Mohamed Abdelsalam, Maha J Genet Eng Biotechnol Research BACKGROUND: Schizophrenia is a chronic mental disorder with different symptoms. The environmental and genetic factors are suggested to be the etiology of schizophrenia. However, the exact cause and pathogenesis of schizophrenia are still unclear. Different studies suggested that the immune system may have a role in schizophrenia. A genetic study found a relation between the disease and the HLA region on the sixth chromosome. Regulatory T cells (Treg) have a role in the regulation of immune response, especially the balance between TH1 and TH2 cells. The FOXP3 protein is a key regulator for Treg cell’s functions. FOXP3 is a transcriptional factor, and its gene is present on the short arm of the X chromosome. The selected SNPs present in the promoter region which act as binding sites for transcriptional factors. This study investigated FOXP3 gene polymorphisms (rs3761548, rs3761549, and rs2232365) in Egyptian patients with schizophrenia. There are no previous studies about the association of FOXP3 gene polymorphisms with schizophrenia. The three selected single-nucleotide polymorphisms (SNPs) were investigated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for 125 schizophrenia patients and 160 healthy controls. The Positive and Negative Syndrome Scale (PANSS) was used to evaluate patients with schizophrenia. RESULTS: No significant associations were found between schizophrenia patients and healthy controls for the alleles and genotypes of the selected SNPs (P-value > 0.05). However, a significant association with ACC and ATC haplotypes was detected (P-value 0.001). No significant association was detected between the PANSS score and any of the studied SNPs. CONCLUSION: The ATC haplotype of rs2232365, rs3761549, and rs3761548 could be considered a risk factor for schizophrenia in Egyptian patients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43141-022-00371-y. Springer Berlin Heidelberg 2022-05-31 /pmc/articles/PMC9156649/ /pubmed/35641708 http://dx.doi.org/10.1186/s43141-022-00371-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Mostafa, Maged Fathy, Aya Ahmed Elwasify, Mohamed Abdelsalam, Maha Analysis of selected polymorphisms in FOXP3 gene in a cohort of Egyptian patients with schizophrenia |
title | Analysis of selected polymorphisms in FOXP3 gene in a cohort of Egyptian patients with schizophrenia |
title_full | Analysis of selected polymorphisms in FOXP3 gene in a cohort of Egyptian patients with schizophrenia |
title_fullStr | Analysis of selected polymorphisms in FOXP3 gene in a cohort of Egyptian patients with schizophrenia |
title_full_unstemmed | Analysis of selected polymorphisms in FOXP3 gene in a cohort of Egyptian patients with schizophrenia |
title_short | Analysis of selected polymorphisms in FOXP3 gene in a cohort of Egyptian patients with schizophrenia |
title_sort | analysis of selected polymorphisms in foxp3 gene in a cohort of egyptian patients with schizophrenia |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9156649/ https://www.ncbi.nlm.nih.gov/pubmed/35641708 http://dx.doi.org/10.1186/s43141-022-00371-y |
work_keys_str_mv | AT mostafamaged analysisofselectedpolymorphismsinfoxp3geneinacohortofegyptianpatientswithschizophrenia AT fathyayaahmed analysisofselectedpolymorphismsinfoxp3geneinacohortofegyptianpatientswithschizophrenia AT elwasifymohamed analysisofselectedpolymorphismsinfoxp3geneinacohortofegyptianpatientswithschizophrenia AT abdelsalammaha analysisofselectedpolymorphismsinfoxp3geneinacohortofegyptianpatientswithschizophrenia |