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Engineering efficient termination of bacteriophage T7 RNA polymerase transcription

The bacteriophage T7 expression system is one of the most prominent transcription systems used in biotechnology and molecular-level research. However, T7 RNA polymerase is prone to read-through transcription due to its high processivity. As a consequence, enforcing efficient transcriptional terminat...

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Detalles Bibliográficos
Autores principales:	Calvopina-Chavez, Diana G, Gardner, Mikaela A, Griffitts, Joel S
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2022
Materias:	Investigation
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157156/ https://www.ncbi.nlm.nih.gov/pubmed/35348690 http://dx.doi.org/10.1093/g3journal/jkac070

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