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Highly efficient site-specific integration of DNA fragments into the honeybee genome using CRISPR/Cas9
Functional genetic studies in honeybees have been limited to transposon mediated transformation and site directed mutagenesis tools. However, site- and sequence-specific manipulations that insert DNA fragments or replace sequences at specific target sites are lacking. Such tools would enable the tag...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157169/ https://www.ncbi.nlm.nih.gov/pubmed/35536186 http://dx.doi.org/10.1093/g3journal/jkac098 |