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Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19

Severe COVID-19 in children is rare, but the reasons underlying are unclear. Profound alterations in T cell responses have been well characterized in the course of adult severe COVID-19, but little is known about the T cell function in children with COVID-19. Here, we made three major observations i...

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Autores principales: Russo, Constanza, Raiden, Silvina, Algieri, Silvia, De Carli, Norberto, Davenport, Carolina, Sarli, Mariam, Bruera, María José, Seery, Vanesa, Sananez, Inés, Simaz, Nancy, Bayle, Carola, Nivela, Valeria, Ferrero, Fernando, Geffner, Jorge, Arruvito, Lourdes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157541/
https://www.ncbi.nlm.nih.gov/pubmed/35663467
http://dx.doi.org/10.3389/fcimb.2022.893044
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author Russo, Constanza
Raiden, Silvina
Algieri, Silvia
De Carli, Norberto
Davenport, Carolina
Sarli, Mariam
Bruera, María José
Seery, Vanesa
Sananez, Inés
Simaz, Nancy
Bayle, Carola
Nivela, Valeria
Ferrero, Fernando
Geffner, Jorge
Arruvito, Lourdes
author_facet Russo, Constanza
Raiden, Silvina
Algieri, Silvia
De Carli, Norberto
Davenport, Carolina
Sarli, Mariam
Bruera, María José
Seery, Vanesa
Sananez, Inés
Simaz, Nancy
Bayle, Carola
Nivela, Valeria
Ferrero, Fernando
Geffner, Jorge
Arruvito, Lourdes
author_sort Russo, Constanza
collection PubMed
description Severe COVID-19 in children is rare, but the reasons underlying are unclear. Profound alterations in T cell responses have been well characterized in the course of adult severe COVID-19, but little is known about the T cell function in children with COVID-19. Here, we made three major observations in a cohort of symptomatic children with acute COVID-19: 1) a reduced frequency of circulating FoxP3+ regulatory T cells, 2) the prevalence of a TH17 polarizing microenvironment characterized by high plasma levels of IL-6, IL-23, and IL17A, and an increased frequency of CD4+ T cells expressing ROR-γt, the master regulator of TH17 development, and 3) high plasma levels of ATP together with an increased expression of the P2X7 receptor. Moreover, that plasma levels of ATP displayed an inverse correlation with the frequency of regulatory T cells but a positive correlation with the frequency of CD4+ T cells positive for the expression of ROR-γt. Collectively, our data indicate an imbalance in CD4+ T cell profiles during pediatric COVID-19 that might favor the course of inflammatory processes. This finding also suggests a possible role for the extracellular ATP in the acquisition of an inflammatory signature by the T cell compartment offering a novel understanding of the involved mechanisms.
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spelling pubmed-91575412022-06-02 Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19 Russo, Constanza Raiden, Silvina Algieri, Silvia De Carli, Norberto Davenport, Carolina Sarli, Mariam Bruera, María José Seery, Vanesa Sananez, Inés Simaz, Nancy Bayle, Carola Nivela, Valeria Ferrero, Fernando Geffner, Jorge Arruvito, Lourdes Front Cell Infect Microbiol Cellular and Infection Microbiology Severe COVID-19 in children is rare, but the reasons underlying are unclear. Profound alterations in T cell responses have been well characterized in the course of adult severe COVID-19, but little is known about the T cell function in children with COVID-19. Here, we made three major observations in a cohort of symptomatic children with acute COVID-19: 1) a reduced frequency of circulating FoxP3+ regulatory T cells, 2) the prevalence of a TH17 polarizing microenvironment characterized by high plasma levels of IL-6, IL-23, and IL17A, and an increased frequency of CD4+ T cells expressing ROR-γt, the master regulator of TH17 development, and 3) high plasma levels of ATP together with an increased expression of the P2X7 receptor. Moreover, that plasma levels of ATP displayed an inverse correlation with the frequency of regulatory T cells but a positive correlation with the frequency of CD4+ T cells positive for the expression of ROR-γt. Collectively, our data indicate an imbalance in CD4+ T cell profiles during pediatric COVID-19 that might favor the course of inflammatory processes. This finding also suggests a possible role for the extracellular ATP in the acquisition of an inflammatory signature by the T cell compartment offering a novel understanding of the involved mechanisms. Frontiers Media S.A. 2022-05-18 /pmc/articles/PMC9157541/ /pubmed/35663467 http://dx.doi.org/10.3389/fcimb.2022.893044 Text en Copyright © 2022 Russo, Raiden, Algieri, De Carli, Davenport, Sarli, Bruera, Seery, Sananez, Simaz, Bayle, Nivela, Ferrero, Geffner and Arruvito https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Russo, Constanza
Raiden, Silvina
Algieri, Silvia
De Carli, Norberto
Davenport, Carolina
Sarli, Mariam
Bruera, María José
Seery, Vanesa
Sananez, Inés
Simaz, Nancy
Bayle, Carola
Nivela, Valeria
Ferrero, Fernando
Geffner, Jorge
Arruvito, Lourdes
Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19
title Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19
title_full Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19
title_fullStr Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19
title_full_unstemmed Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19
title_short Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19
title_sort extracellular atp and imbalance of cd4+ t cell compartment in pediatric covid-19
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157541/
https://www.ncbi.nlm.nih.gov/pubmed/35663467
http://dx.doi.org/10.3389/fcimb.2022.893044
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