Cargando…
Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19
Severe COVID-19 in children is rare, but the reasons underlying are unclear. Profound alterations in T cell responses have been well characterized in the course of adult severe COVID-19, but little is known about the T cell function in children with COVID-19. Here, we made three major observations i...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157541/ https://www.ncbi.nlm.nih.gov/pubmed/35663467 http://dx.doi.org/10.3389/fcimb.2022.893044 |
_version_ | 1784718655621169152 |
---|---|
author | Russo, Constanza Raiden, Silvina Algieri, Silvia De Carli, Norberto Davenport, Carolina Sarli, Mariam Bruera, María José Seery, Vanesa Sananez, Inés Simaz, Nancy Bayle, Carola Nivela, Valeria Ferrero, Fernando Geffner, Jorge Arruvito, Lourdes |
author_facet | Russo, Constanza Raiden, Silvina Algieri, Silvia De Carli, Norberto Davenport, Carolina Sarli, Mariam Bruera, María José Seery, Vanesa Sananez, Inés Simaz, Nancy Bayle, Carola Nivela, Valeria Ferrero, Fernando Geffner, Jorge Arruvito, Lourdes |
author_sort | Russo, Constanza |
collection | PubMed |
description | Severe COVID-19 in children is rare, but the reasons underlying are unclear. Profound alterations in T cell responses have been well characterized in the course of adult severe COVID-19, but little is known about the T cell function in children with COVID-19. Here, we made three major observations in a cohort of symptomatic children with acute COVID-19: 1) a reduced frequency of circulating FoxP3+ regulatory T cells, 2) the prevalence of a TH17 polarizing microenvironment characterized by high plasma levels of IL-6, IL-23, and IL17A, and an increased frequency of CD4+ T cells expressing ROR-γt, the master regulator of TH17 development, and 3) high plasma levels of ATP together with an increased expression of the P2X7 receptor. Moreover, that plasma levels of ATP displayed an inverse correlation with the frequency of regulatory T cells but a positive correlation with the frequency of CD4+ T cells positive for the expression of ROR-γt. Collectively, our data indicate an imbalance in CD4+ T cell profiles during pediatric COVID-19 that might favor the course of inflammatory processes. This finding also suggests a possible role for the extracellular ATP in the acquisition of an inflammatory signature by the T cell compartment offering a novel understanding of the involved mechanisms. |
format | Online Article Text |
id | pubmed-9157541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91575412022-06-02 Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19 Russo, Constanza Raiden, Silvina Algieri, Silvia De Carli, Norberto Davenport, Carolina Sarli, Mariam Bruera, María José Seery, Vanesa Sananez, Inés Simaz, Nancy Bayle, Carola Nivela, Valeria Ferrero, Fernando Geffner, Jorge Arruvito, Lourdes Front Cell Infect Microbiol Cellular and Infection Microbiology Severe COVID-19 in children is rare, but the reasons underlying are unclear. Profound alterations in T cell responses have been well characterized in the course of adult severe COVID-19, but little is known about the T cell function in children with COVID-19. Here, we made three major observations in a cohort of symptomatic children with acute COVID-19: 1) a reduced frequency of circulating FoxP3+ regulatory T cells, 2) the prevalence of a TH17 polarizing microenvironment characterized by high plasma levels of IL-6, IL-23, and IL17A, and an increased frequency of CD4+ T cells expressing ROR-γt, the master regulator of TH17 development, and 3) high plasma levels of ATP together with an increased expression of the P2X7 receptor. Moreover, that plasma levels of ATP displayed an inverse correlation with the frequency of regulatory T cells but a positive correlation with the frequency of CD4+ T cells positive for the expression of ROR-γt. Collectively, our data indicate an imbalance in CD4+ T cell profiles during pediatric COVID-19 that might favor the course of inflammatory processes. This finding also suggests a possible role for the extracellular ATP in the acquisition of an inflammatory signature by the T cell compartment offering a novel understanding of the involved mechanisms. Frontiers Media S.A. 2022-05-18 /pmc/articles/PMC9157541/ /pubmed/35663467 http://dx.doi.org/10.3389/fcimb.2022.893044 Text en Copyright © 2022 Russo, Raiden, Algieri, De Carli, Davenport, Sarli, Bruera, Seery, Sananez, Simaz, Bayle, Nivela, Ferrero, Geffner and Arruvito https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Russo, Constanza Raiden, Silvina Algieri, Silvia De Carli, Norberto Davenport, Carolina Sarli, Mariam Bruera, María José Seery, Vanesa Sananez, Inés Simaz, Nancy Bayle, Carola Nivela, Valeria Ferrero, Fernando Geffner, Jorge Arruvito, Lourdes Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19 |
title | Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19 |
title_full | Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19 |
title_fullStr | Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19 |
title_full_unstemmed | Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19 |
title_short | Extracellular ATP and Imbalance of CD4+ T Cell Compartment in Pediatric COVID-19 |
title_sort | extracellular atp and imbalance of cd4+ t cell compartment in pediatric covid-19 |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157541/ https://www.ncbi.nlm.nih.gov/pubmed/35663467 http://dx.doi.org/10.3389/fcimb.2022.893044 |
work_keys_str_mv | AT russoconstanza extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT raidensilvina extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT algierisilvia extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT decarlinorberto extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT davenportcarolina extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT sarlimariam extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT brueramariajose extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT seeryvanesa extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT sananezines extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT simaznancy extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT baylecarola extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT nivelavaleria extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT ferrerofernando extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT geffnerjorge extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 AT arruvitolourdes extracellularatpandimbalanceofcd4tcellcompartmentinpediatriccovid19 |