Cargando…
Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics
Elasticity and bio-adhesiveness of circulating tumor cells (CTCs) are important biomarkers of cancer. CTCs are rare in blood, thus their capture and atomic force microscopy (AFM)-based biomechanical characterization require use of multifunctional microfluidic device. Here, we describe procedures for...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157559/ https://www.ncbi.nlm.nih.gov/pubmed/35664257 http://dx.doi.org/10.1016/j.xpro.2022.101433 |
_version_ | 1784718659851124736 |
---|---|
author | Deliorman, Muhammedin Glia, Ayoub Qasaimeh, Mohammad A. |
author_facet | Deliorman, Muhammedin Glia, Ayoub Qasaimeh, Mohammad A. |
author_sort | Deliorman, Muhammedin |
collection | PubMed |
description | Elasticity and bio-adhesiveness of circulating tumor cells (CTCs) are important biomarkers of cancer. CTCs are rare in blood, thus their capture and atomic force microscopy (AFM)-based biomechanical characterization require use of multifunctional microfluidic device. Here, we describe procedures for fabrication of such device, AFM-Chip, and give details on its use in affinity-based CTC capture, and integration with AFM via reversable physical assembly. In the AFM-Chip, CTC capture is efficient, and transition to AFM characterization is seamless with minimal cell loss. For complete details on the use and execution of this protocol, please refer to Deliorman et al. (2020). |
format | Online Article Text |
id | pubmed-9157559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-91575592022-06-02 Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics Deliorman, Muhammedin Glia, Ayoub Qasaimeh, Mohammad A. STAR Protoc Protocol Elasticity and bio-adhesiveness of circulating tumor cells (CTCs) are important biomarkers of cancer. CTCs are rare in blood, thus their capture and atomic force microscopy (AFM)-based biomechanical characterization require use of multifunctional microfluidic device. Here, we describe procedures for fabrication of such device, AFM-Chip, and give details on its use in affinity-based CTC capture, and integration with AFM via reversable physical assembly. In the AFM-Chip, CTC capture is efficient, and transition to AFM characterization is seamless with minimal cell loss. For complete details on the use and execution of this protocol, please refer to Deliorman et al. (2020). Elsevier 2022-05-27 /pmc/articles/PMC9157559/ /pubmed/35664257 http://dx.doi.org/10.1016/j.xpro.2022.101433 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Deliorman, Muhammedin Glia, Ayoub Qasaimeh, Mohammad A. Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics |
title | Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics |
title_full | Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics |
title_fullStr | Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics |
title_full_unstemmed | Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics |
title_short | Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics |
title_sort | characterizing circulating tumor cells using affinity-based microfluidic capture and afm-based biomechanics |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157559/ https://www.ncbi.nlm.nih.gov/pubmed/35664257 http://dx.doi.org/10.1016/j.xpro.2022.101433 |
work_keys_str_mv | AT deliormanmuhammedin characterizingcirculatingtumorcellsusingaffinitybasedmicrofluidiccaptureandafmbasedbiomechanics AT gliaayoub characterizingcirculatingtumorcellsusingaffinitybasedmicrofluidiccaptureandafmbasedbiomechanics AT qasaimehmohammada characterizingcirculatingtumorcellsusingaffinitybasedmicrofluidiccaptureandafmbasedbiomechanics |