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Landscape of Metabolic Fingerprinting for Diagnosis and Risk Stratification of Sepsis

BACKGROUND: Sepsis and septic shock, a subset of sepsis with higher risk stratification, are hallmarked by high mortality rates and necessitated early and accurate biomarkers. METHODS: Untargeted metabolomic analysis was performed to compare the metabolic features between the sepsis and control syst...

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Autores principales: Lu, Geng, Zhou, Jiawei, Yang, Ting, Li, Jin, Jiang, Xinrui, Zhang, Wenjun, Gu, Shuangshuang, Wang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159301/
https://www.ncbi.nlm.nih.gov/pubmed/35663956
http://dx.doi.org/10.3389/fimmu.2022.883628
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author Lu, Geng
Zhou, Jiawei
Yang, Ting
Li, Jin
Jiang, Xinrui
Zhang, Wenjun
Gu, Shuangshuang
Wang, Jun
author_facet Lu, Geng
Zhou, Jiawei
Yang, Ting
Li, Jin
Jiang, Xinrui
Zhang, Wenjun
Gu, Shuangshuang
Wang, Jun
author_sort Lu, Geng
collection PubMed
description BACKGROUND: Sepsis and septic shock, a subset of sepsis with higher risk stratification, are hallmarked by high mortality rates and necessitated early and accurate biomarkers. METHODS: Untargeted metabolomic analysis was performed to compare the metabolic features between the sepsis and control systemic inflammatory response syndrome (SIRS) groups in discovery cohort, and potential metabolic biomarkers were selected and quantified using multiple reaction monitoring based target metabolite detection method. RESULTS: Differentially expressed metabolites including 46 metabolites in positive electrospray ionization (ESI) ion mode, 22 metabolites in negative ESI ion mode, and 4 metabolites with dual mode between sepsis and SIRS were identified and revealed. Metabolites 5-Oxoproline, L-Kynurenine and Leukotriene D4 were selected based on least absolute shrinkage and selection operator regularization logistic regression and differential expressed between sepsis and septic shock group in the training and test cohorts. Respective risk scores for sepsis and septic shock based on a 3-metabolite fingerprint classifier were established to distinguish sepsis from SIRS, septic shock from sepsis. Significant relationship between developed sepsis risk scores, septic shock risk scores and Sequential (sepsis-related) Organ Failure Assessment (SOFA), procalcitonin (PCT) and lactic acid were observed. CONCLUSIONS: Collectively, our findings demonstrated that the characteristics of plasma metabolites not only manifest phenotypic variation in sepsis onset and risk stratification of sepsis but also enable individualized treatment and improve current therapeutic strategies.
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spelling pubmed-91593012022-06-02 Landscape of Metabolic Fingerprinting for Diagnosis and Risk Stratification of Sepsis Lu, Geng Zhou, Jiawei Yang, Ting Li, Jin Jiang, Xinrui Zhang, Wenjun Gu, Shuangshuang Wang, Jun Front Immunol Immunology BACKGROUND: Sepsis and septic shock, a subset of sepsis with higher risk stratification, are hallmarked by high mortality rates and necessitated early and accurate biomarkers. METHODS: Untargeted metabolomic analysis was performed to compare the metabolic features between the sepsis and control systemic inflammatory response syndrome (SIRS) groups in discovery cohort, and potential metabolic biomarkers were selected and quantified using multiple reaction monitoring based target metabolite detection method. RESULTS: Differentially expressed metabolites including 46 metabolites in positive electrospray ionization (ESI) ion mode, 22 metabolites in negative ESI ion mode, and 4 metabolites with dual mode between sepsis and SIRS were identified and revealed. Metabolites 5-Oxoproline, L-Kynurenine and Leukotriene D4 were selected based on least absolute shrinkage and selection operator regularization logistic regression and differential expressed between sepsis and septic shock group in the training and test cohorts. Respective risk scores for sepsis and septic shock based on a 3-metabolite fingerprint classifier were established to distinguish sepsis from SIRS, septic shock from sepsis. Significant relationship between developed sepsis risk scores, septic shock risk scores and Sequential (sepsis-related) Organ Failure Assessment (SOFA), procalcitonin (PCT) and lactic acid were observed. CONCLUSIONS: Collectively, our findings demonstrated that the characteristics of plasma metabolites not only manifest phenotypic variation in sepsis onset and risk stratification of sepsis but also enable individualized treatment and improve current therapeutic strategies. Frontiers Media S.A. 2022-05-18 /pmc/articles/PMC9159301/ /pubmed/35663956 http://dx.doi.org/10.3389/fimmu.2022.883628 Text en Copyright © 2022 Lu, Zhou, Yang, Li, Jiang, Zhang, Gu and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Lu, Geng
Zhou, Jiawei
Yang, Ting
Li, Jin
Jiang, Xinrui
Zhang, Wenjun
Gu, Shuangshuang
Wang, Jun
Landscape of Metabolic Fingerprinting for Diagnosis and Risk Stratification of Sepsis
title Landscape of Metabolic Fingerprinting for Diagnosis and Risk Stratification of Sepsis
title_full Landscape of Metabolic Fingerprinting for Diagnosis and Risk Stratification of Sepsis
title_fullStr Landscape of Metabolic Fingerprinting for Diagnosis and Risk Stratification of Sepsis
title_full_unstemmed Landscape of Metabolic Fingerprinting for Diagnosis and Risk Stratification of Sepsis
title_short Landscape of Metabolic Fingerprinting for Diagnosis and Risk Stratification of Sepsis
title_sort landscape of metabolic fingerprinting for diagnosis and risk stratification of sepsis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159301/
https://www.ncbi.nlm.nih.gov/pubmed/35663956
http://dx.doi.org/10.3389/fimmu.2022.883628
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