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Bioprinted Gelatin-Recombinant Type III Collagen Hydrogel Promotes Wound Healing
Artificial skins are biomaterials that can replace the lost skin or promote the regeneration of damaged skin. Skin regenerative biomaterials are highly applauded because they can exempt patients with severe burns from the painful procedure of autologous skin transplantation. Notwithstanding decades...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Whioce Publishing Pte. Ltd.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159484/ https://www.ncbi.nlm.nih.gov/pubmed/35669327 http://dx.doi.org/10.18063/ijb.v8i2.517 |
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author | Huang, Jianghong Lei, Xiaoling Huang, Zhiwang Rong, Zhibin Li, Haihang Xie, Yixin Duan, Li Xiong, Jianyi Wang, Daping Zhu, Shihui Liang, Yujie Wang, Jianhao Xia, Jiang |
author_facet | Huang, Jianghong Lei, Xiaoling Huang, Zhiwang Rong, Zhibin Li, Haihang Xie, Yixin Duan, Li Xiong, Jianyi Wang, Daping Zhu, Shihui Liang, Yujie Wang, Jianhao Xia, Jiang |
author_sort | Huang, Jianghong |
collection | PubMed |
description | Artificial skins are biomaterials that can replace the lost skin or promote the regeneration of damaged skin. Skin regenerative biomaterials are highly applauded because they can exempt patients with severe burns from the painful procedure of autologous skin transplantation. Notwithstanding decades of research, biocompatible, degradable, and printable biomaterials that can effectively promote skin regeneration as a transplantation replacement in clinical use are still scarce. Here, we report one type of all-protein hydrogel material as the product of the enzymatic crosslinking reaction of gelatin and a recombinant type III collagen (rColIII) protein. Doping the rColIII protein in gelatin reduces the inflammatory response as an implant underneath the skin. The all-protein hydrogel can be bioprinted as scaffolds to support the growth and proliferation of 3T3 fibroblast cells. The hydrogel used as a wound dressing promotes wound healing in a rat model of skin damage, showing a faster and healthier recovery than the controls. The rColIII protein in the hydrogel has been shown to play a critical role in skin regeneration. Altogether, this work manifests the development of all-protein gelatin-rColIII hydrogel and demonstrates its use in wound healing. The gelatin-collagen hydrogel wound dressing thereby may become a promising treatment of severe wounds in the future. |
format | Online Article Text |
id | pubmed-9159484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Whioce Publishing Pte. Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91594842022-06-05 Bioprinted Gelatin-Recombinant Type III Collagen Hydrogel Promotes Wound Healing Huang, Jianghong Lei, Xiaoling Huang, Zhiwang Rong, Zhibin Li, Haihang Xie, Yixin Duan, Li Xiong, Jianyi Wang, Daping Zhu, Shihui Liang, Yujie Wang, Jianhao Xia, Jiang Int J Bioprint Research Article Artificial skins are biomaterials that can replace the lost skin or promote the regeneration of damaged skin. Skin regenerative biomaterials are highly applauded because they can exempt patients with severe burns from the painful procedure of autologous skin transplantation. Notwithstanding decades of research, biocompatible, degradable, and printable biomaterials that can effectively promote skin regeneration as a transplantation replacement in clinical use are still scarce. Here, we report one type of all-protein hydrogel material as the product of the enzymatic crosslinking reaction of gelatin and a recombinant type III collagen (rColIII) protein. Doping the rColIII protein in gelatin reduces the inflammatory response as an implant underneath the skin. The all-protein hydrogel can be bioprinted as scaffolds to support the growth and proliferation of 3T3 fibroblast cells. The hydrogel used as a wound dressing promotes wound healing in a rat model of skin damage, showing a faster and healthier recovery than the controls. The rColIII protein in the hydrogel has been shown to play a critical role in skin regeneration. Altogether, this work manifests the development of all-protein gelatin-rColIII hydrogel and demonstrates its use in wound healing. The gelatin-collagen hydrogel wound dressing thereby may become a promising treatment of severe wounds in the future. Whioce Publishing Pte. Ltd. 2022-02-24 /pmc/articles/PMC9159484/ /pubmed/35669327 http://dx.doi.org/10.18063/ijb.v8i2.517 Text en Copyright: © 2022 Huang, et al. https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Attribution-NonCommercial 4.0 International 4.0 (CC BY-NC 4.0), which permits all non-commercial use, distribution, and reproduction in any medium provided the original work is properly cited. |
spellingShingle | Research Article Huang, Jianghong Lei, Xiaoling Huang, Zhiwang Rong, Zhibin Li, Haihang Xie, Yixin Duan, Li Xiong, Jianyi Wang, Daping Zhu, Shihui Liang, Yujie Wang, Jianhao Xia, Jiang Bioprinted Gelatin-Recombinant Type III Collagen Hydrogel Promotes Wound Healing |
title | Bioprinted Gelatin-Recombinant Type III Collagen Hydrogel Promotes Wound Healing |
title_full | Bioprinted Gelatin-Recombinant Type III Collagen Hydrogel Promotes Wound Healing |
title_fullStr | Bioprinted Gelatin-Recombinant Type III Collagen Hydrogel Promotes Wound Healing |
title_full_unstemmed | Bioprinted Gelatin-Recombinant Type III Collagen Hydrogel Promotes Wound Healing |
title_short | Bioprinted Gelatin-Recombinant Type III Collagen Hydrogel Promotes Wound Healing |
title_sort | bioprinted gelatin-recombinant type iii collagen hydrogel promotes wound healing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159484/ https://www.ncbi.nlm.nih.gov/pubmed/35669327 http://dx.doi.org/10.18063/ijb.v8i2.517 |
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