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Diagnostic accuracy of non-invasive detection of SARS-CoV-2 infection by canine olfaction

BACKGROUND: Throughout the COVID-19 pandemic, testing individuals remains a key action. One approach to rapid testing is to consider the olfactory capacities of trained detection dogs. METHODS: Prospective cohort study in two community COVID-19 screening centers. Two nasopharyngeal swabs (NPS), one...

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Autores principales: GRANDJEAN, Dominique, ELIE, Caroline, GALLET, Capucine, JULIEN, Clotilde, ROGER, Vinciane, DESQUILBET, Loïc, ALVERGNAT, Guillaume, DELARUE, Séverine, GABASSI, Audrey, MINIER, Marine, CHOUPEAUX, Laure, KERNEIS, Solen, DELAUGERRE, Constance, LE GOFF, Jérôme, TRELUYER, Jean-Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159600/
https://www.ncbi.nlm.nih.gov/pubmed/35648737
http://dx.doi.org/10.1371/journal.pone.0268382
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author GRANDJEAN, Dominique
ELIE, Caroline
GALLET, Capucine
JULIEN, Clotilde
ROGER, Vinciane
DESQUILBET, Loïc
ALVERGNAT, Guillaume
DELARUE, Séverine
GABASSI, Audrey
MINIER, Marine
CHOUPEAUX, Laure
KERNEIS, Solen
DELAUGERRE, Constance
LE GOFF, Jérôme
TRELUYER, Jean-Marc
author_facet GRANDJEAN, Dominique
ELIE, Caroline
GALLET, Capucine
JULIEN, Clotilde
ROGER, Vinciane
DESQUILBET, Loïc
ALVERGNAT, Guillaume
DELARUE, Séverine
GABASSI, Audrey
MINIER, Marine
CHOUPEAUX, Laure
KERNEIS, Solen
DELAUGERRE, Constance
LE GOFF, Jérôme
TRELUYER, Jean-Marc
author_sort GRANDJEAN, Dominique
collection PubMed
description BACKGROUND: Throughout the COVID-19 pandemic, testing individuals remains a key action. One approach to rapid testing is to consider the olfactory capacities of trained detection dogs. METHODS: Prospective cohort study in two community COVID-19 screening centers. Two nasopharyngeal swabs (NPS), one saliva and one sweat samples were simultaneously collected. The dog handlers (and the dogs…) were blinded with regards to the Covid status. The diagnostic accuracy of non-invasive detection of SARS-CoV-2 infection by canine olfaction was assessed as compared to nasopharyngeal RT-PCR as the reference standard, saliva RT-PCR and nasopharyngeal antigen testing. RESULTS: 335 ambulatory adults (143 symptomatic and 192 asymptomatic) were included. Overall, 109/335 participants tested positive on nasopharyngeal RT-PCR either in symptomatic (78/143) or in asymptomatic participants (31/192). The overall sensitivity of canine detection was 97% (95% CI, 92 to 99) and even reached 100% (95% CI, 89 to 100) in asymptomatic individuals compared to NPS RT-PCR. The specificity was 91% (95% CI, 72 to 91), reaching 94% (95% CI, 90 to 97) for asymptomatic individuals. The sensitivity of canine detection was higher than that of nasopharyngeal antigen testing (97% CI: 91 to 99 versus 84% CI: 74 to 90, p = 0.006), but the specificity was lower (90% CI: 84 to 95 versus 97% CI: 93 to 99, p = 0.016). CONCLUSIONS: Non-invasive detection of SARS-CoV-2 infection by canine olfaction could be one alternative to NPS RT-PCR when it is necessary to obtain a result very quickly according to the same indications as antigenic tests in the context of mass screening.
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spelling pubmed-91596002022-06-02 Diagnostic accuracy of non-invasive detection of SARS-CoV-2 infection by canine olfaction GRANDJEAN, Dominique ELIE, Caroline GALLET, Capucine JULIEN, Clotilde ROGER, Vinciane DESQUILBET, Loïc ALVERGNAT, Guillaume DELARUE, Séverine GABASSI, Audrey MINIER, Marine CHOUPEAUX, Laure KERNEIS, Solen DELAUGERRE, Constance LE GOFF, Jérôme TRELUYER, Jean-Marc PLoS One Research Article BACKGROUND: Throughout the COVID-19 pandemic, testing individuals remains a key action. One approach to rapid testing is to consider the olfactory capacities of trained detection dogs. METHODS: Prospective cohort study in two community COVID-19 screening centers. Two nasopharyngeal swabs (NPS), one saliva and one sweat samples were simultaneously collected. The dog handlers (and the dogs…) were blinded with regards to the Covid status. The diagnostic accuracy of non-invasive detection of SARS-CoV-2 infection by canine olfaction was assessed as compared to nasopharyngeal RT-PCR as the reference standard, saliva RT-PCR and nasopharyngeal antigen testing. RESULTS: 335 ambulatory adults (143 symptomatic and 192 asymptomatic) were included. Overall, 109/335 participants tested positive on nasopharyngeal RT-PCR either in symptomatic (78/143) or in asymptomatic participants (31/192). The overall sensitivity of canine detection was 97% (95% CI, 92 to 99) and even reached 100% (95% CI, 89 to 100) in asymptomatic individuals compared to NPS RT-PCR. The specificity was 91% (95% CI, 72 to 91), reaching 94% (95% CI, 90 to 97) for asymptomatic individuals. The sensitivity of canine detection was higher than that of nasopharyngeal antigen testing (97% CI: 91 to 99 versus 84% CI: 74 to 90, p = 0.006), but the specificity was lower (90% CI: 84 to 95 versus 97% CI: 93 to 99, p = 0.016). CONCLUSIONS: Non-invasive detection of SARS-CoV-2 infection by canine olfaction could be one alternative to NPS RT-PCR when it is necessary to obtain a result very quickly according to the same indications as antigenic tests in the context of mass screening. Public Library of Science 2022-06-01 /pmc/articles/PMC9159600/ /pubmed/35648737 http://dx.doi.org/10.1371/journal.pone.0268382 Text en © 2022 GRANDJEAN et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
GRANDJEAN, Dominique
ELIE, Caroline
GALLET, Capucine
JULIEN, Clotilde
ROGER, Vinciane
DESQUILBET, Loïc
ALVERGNAT, Guillaume
DELARUE, Séverine
GABASSI, Audrey
MINIER, Marine
CHOUPEAUX, Laure
KERNEIS, Solen
DELAUGERRE, Constance
LE GOFF, Jérôme
TRELUYER, Jean-Marc
Diagnostic accuracy of non-invasive detection of SARS-CoV-2 infection by canine olfaction
title Diagnostic accuracy of non-invasive detection of SARS-CoV-2 infection by canine olfaction
title_full Diagnostic accuracy of non-invasive detection of SARS-CoV-2 infection by canine olfaction
title_fullStr Diagnostic accuracy of non-invasive detection of SARS-CoV-2 infection by canine olfaction
title_full_unstemmed Diagnostic accuracy of non-invasive detection of SARS-CoV-2 infection by canine olfaction
title_short Diagnostic accuracy of non-invasive detection of SARS-CoV-2 infection by canine olfaction
title_sort diagnostic accuracy of non-invasive detection of sars-cov-2 infection by canine olfaction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159600/
https://www.ncbi.nlm.nih.gov/pubmed/35648737
http://dx.doi.org/10.1371/journal.pone.0268382
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