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Platform for isolation and characterization of SARS-CoV-2 variants enables rapid characterization of Omicron in Australia
Genetically distinct variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged since the start of the COVID-19 pandemic. Over this period, we developed a rapid platform (R-20) for viral isolation and characterization using primary remnant diagnostic swabs. This, combined...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159941/ https://www.ncbi.nlm.nih.gov/pubmed/35637329 http://dx.doi.org/10.1038/s41564-022-01135-7 |
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| author | Aggarwal, Anupriya Stella, Alberto Ospina Walker, Gregory Akerman, Anouschka Esneau, Camille Milogiannakis, Vanessa Burnett, Deborah L. McAllery, Samantha Silva, Mariana Ruiz Lu, Yonghui Foster, Charles S. P. Brilot, Fabienne Pillay, Aleha Van Hal, Sabastiaan Mathivanan, Vennila Fichter, Christina Kindinger, Andrea Hoppe, Alexandra Carey Munier, Mee Ling Amatayakul-Chantler, Supavadee Roth, Nathan Coppola, Germano Symonds, Geoff P. Schofield, Peter Jackson, Jennifer Lenthall, Helen Henry, Jake Y. Mazigi, Ohan Jäck, Hans-Martin Davenport, Miles P. Darley, David R. Matthews, Gail V. Khoury, David S. Cromer, Deborah Goodnow, Christopher C. Christ, Daniel Robosa, Roselle Starck, Damien J. Bartlett, Nathan W. Rawlinson, William D. Kelleher, Anthony D. Turville, Stuart G. |
| author_facet | Aggarwal, Anupriya Stella, Alberto Ospina Walker, Gregory Akerman, Anouschka Esneau, Camille Milogiannakis, Vanessa Burnett, Deborah L. McAllery, Samantha Silva, Mariana Ruiz Lu, Yonghui Foster, Charles S. P. Brilot, Fabienne Pillay, Aleha Van Hal, Sabastiaan Mathivanan, Vennila Fichter, Christina Kindinger, Andrea Hoppe, Alexandra Carey Munier, Mee Ling Amatayakul-Chantler, Supavadee Roth, Nathan Coppola, Germano Symonds, Geoff P. Schofield, Peter Jackson, Jennifer Lenthall, Helen Henry, Jake Y. Mazigi, Ohan Jäck, Hans-Martin Davenport, Miles P. Darley, David R. Matthews, Gail V. Khoury, David S. Cromer, Deborah Goodnow, Christopher C. Christ, Daniel Robosa, Roselle Starck, Damien J. Bartlett, Nathan W. Rawlinson, William D. Kelleher, Anthony D. Turville, Stuart G. |
| author_sort | Aggarwal, Anupriya |
| collection | PubMed |
| description | Genetically distinct variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged since the start of the COVID-19 pandemic. Over this period, we developed a rapid platform (R-20) for viral isolation and characterization using primary remnant diagnostic swabs. This, combined with quarantine testing and genomics surveillance, enabled the rapid isolation and characterization of all major SARS-CoV-2 variants circulating in Australia in 2021. Our platform facilitated viral variant isolation, rapid resolution of variant fitness using nasopharyngeal swabs and ranking of evasion of neutralizing antibodies. In late 2021, variant of concern Omicron (B1.1.529) emerged. Using our platform, we detected and characterized SARS-CoV-2 VOC Omicron. We show that Omicron effectively evades neutralization antibodies and has a different entry route that is TMPRSS2-independent. Our low-cost platform is available to all and can detect all variants of SARS-CoV-2 studied so far, with the main limitation being that our platform still requires appropriate biocontainment. |
| format | Online Article Text |
| id | pubmed-9159941 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-91599412022-06-03 Platform for isolation and characterization of SARS-CoV-2 variants enables rapid characterization of Omicron in Australia Aggarwal, Anupriya Stella, Alberto Ospina Walker, Gregory Akerman, Anouschka Esneau, Camille Milogiannakis, Vanessa Burnett, Deborah L. McAllery, Samantha Silva, Mariana Ruiz Lu, Yonghui Foster, Charles S. P. Brilot, Fabienne Pillay, Aleha Van Hal, Sabastiaan Mathivanan, Vennila Fichter, Christina Kindinger, Andrea Hoppe, Alexandra Carey Munier, Mee Ling Amatayakul-Chantler, Supavadee Roth, Nathan Coppola, Germano Symonds, Geoff P. Schofield, Peter Jackson, Jennifer Lenthall, Helen Henry, Jake Y. Mazigi, Ohan Jäck, Hans-Martin Davenport, Miles P. Darley, David R. Matthews, Gail V. Khoury, David S. Cromer, Deborah Goodnow, Christopher C. Christ, Daniel Robosa, Roselle Starck, Damien J. Bartlett, Nathan W. Rawlinson, William D. Kelleher, Anthony D. Turville, Stuart G. Nat Microbiol Article Genetically distinct variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged since the start of the COVID-19 pandemic. Over this period, we developed a rapid platform (R-20) for viral isolation and characterization using primary remnant diagnostic swabs. This, combined with quarantine testing and genomics surveillance, enabled the rapid isolation and characterization of all major SARS-CoV-2 variants circulating in Australia in 2021. Our platform facilitated viral variant isolation, rapid resolution of variant fitness using nasopharyngeal swabs and ranking of evasion of neutralizing antibodies. In late 2021, variant of concern Omicron (B1.1.529) emerged. Using our platform, we detected and characterized SARS-CoV-2 VOC Omicron. We show that Omicron effectively evades neutralization antibodies and has a different entry route that is TMPRSS2-independent. Our low-cost platform is available to all and can detect all variants of SARS-CoV-2 studied so far, with the main limitation being that our platform still requires appropriate biocontainment. Nature Publishing Group UK 2022-05-30 2022 /pmc/articles/PMC9159941/ /pubmed/35637329 http://dx.doi.org/10.1038/s41564-022-01135-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Article Aggarwal, Anupriya Stella, Alberto Ospina Walker, Gregory Akerman, Anouschka Esneau, Camille Milogiannakis, Vanessa Burnett, Deborah L. McAllery, Samantha Silva, Mariana Ruiz Lu, Yonghui Foster, Charles S. P. Brilot, Fabienne Pillay, Aleha Van Hal, Sabastiaan Mathivanan, Vennila Fichter, Christina Kindinger, Andrea Hoppe, Alexandra Carey Munier, Mee Ling Amatayakul-Chantler, Supavadee Roth, Nathan Coppola, Germano Symonds, Geoff P. Schofield, Peter Jackson, Jennifer Lenthall, Helen Henry, Jake Y. Mazigi, Ohan Jäck, Hans-Martin Davenport, Miles P. Darley, David R. Matthews, Gail V. Khoury, David S. Cromer, Deborah Goodnow, Christopher C. Christ, Daniel Robosa, Roselle Starck, Damien J. Bartlett, Nathan W. Rawlinson, William D. Kelleher, Anthony D. Turville, Stuart G. Platform for isolation and characterization of SARS-CoV-2 variants enables rapid characterization of Omicron in Australia |
| title | Platform for isolation and characterization of SARS-CoV-2 variants enables rapid characterization of Omicron in Australia |
| title_full | Platform for isolation and characterization of SARS-CoV-2 variants enables rapid characterization of Omicron in Australia |
| title_fullStr | Platform for isolation and characterization of SARS-CoV-2 variants enables rapid characterization of Omicron in Australia |
| title_full_unstemmed | Platform for isolation and characterization of SARS-CoV-2 variants enables rapid characterization of Omicron in Australia |
| title_short | Platform for isolation and characterization of SARS-CoV-2 variants enables rapid characterization of Omicron in Australia |
| title_sort | platform for isolation and characterization of sars-cov-2 variants enables rapid characterization of omicron in australia |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159941/ https://www.ncbi.nlm.nih.gov/pubmed/35637329 http://dx.doi.org/10.1038/s41564-022-01135-7 |
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