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Molecular detection of bacterial contamination in plasma using magnetic-based enrichment
Bacterial contamination of blood products is a major problem in transfusion medicine, in terms of both morbidity and mortality. Platelets (PLTs) are stored at room temperature (under constant agitation) for more than 5 days, and bacteria can thus grow significantly from a low level to high titers. H...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160056/ https://www.ncbi.nlm.nih.gov/pubmed/35650226 http://dx.doi.org/10.1038/s41598-022-12960-5 |
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author | Lee, Jinyeop Abafogi, Abdurhaman Teyib Oh, Sujin Chang, Ho Eun Tepeng, Wu Lee, Daekyu Park, Sungsu Park, Kyoung Un Hong, Yun Ji |
author_facet | Lee, Jinyeop Abafogi, Abdurhaman Teyib Oh, Sujin Chang, Ho Eun Tepeng, Wu Lee, Daekyu Park, Sungsu Park, Kyoung Un Hong, Yun Ji |
author_sort | Lee, Jinyeop |
collection | PubMed |
description | Bacterial contamination of blood products is a major problem in transfusion medicine, in terms of both morbidity and mortality. Platelets (PLTs) are stored at room temperature (under constant agitation) for more than 5 days, and bacteria can thus grow significantly from a low level to high titers. However, conventional methods like blood culture and lateral flow assay have disadvantages such as long detection time, low sensitivity, and the need for a large volume of blood components. We used real-time polymerase chain reaction (PCR) assays with antibiotic-conjugated magnetic nanobeads (MNBs) to detect enriched Gram-positive and -negative bacteria. The MNBs were coated with polyethylene glycol (PEG) to prevent aggregation by blood components. Over 80% of all bacteria were captured by the MNBs, and the levels of detection were 10(1) colony forming unit [CFU]/mL and 10(2) CFU/mL for Gram-positive and -negative bacteria, respectively. The detection time is < 3 h using only small volumes of blood components. Thus, compared to conventional methods, real-time PCR using MNBs allows for rapid detection with high sensitivity using only a small volume of blood components. |
format | Online Article Text |
id | pubmed-9160056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-91600562022-06-03 Molecular detection of bacterial contamination in plasma using magnetic-based enrichment Lee, Jinyeop Abafogi, Abdurhaman Teyib Oh, Sujin Chang, Ho Eun Tepeng, Wu Lee, Daekyu Park, Sungsu Park, Kyoung Un Hong, Yun Ji Sci Rep Article Bacterial contamination of blood products is a major problem in transfusion medicine, in terms of both morbidity and mortality. Platelets (PLTs) are stored at room temperature (under constant agitation) for more than 5 days, and bacteria can thus grow significantly from a low level to high titers. However, conventional methods like blood culture and lateral flow assay have disadvantages such as long detection time, low sensitivity, and the need for a large volume of blood components. We used real-time polymerase chain reaction (PCR) assays with antibiotic-conjugated magnetic nanobeads (MNBs) to detect enriched Gram-positive and -negative bacteria. The MNBs were coated with polyethylene glycol (PEG) to prevent aggregation by blood components. Over 80% of all bacteria were captured by the MNBs, and the levels of detection were 10(1) colony forming unit [CFU]/mL and 10(2) CFU/mL for Gram-positive and -negative bacteria, respectively. The detection time is < 3 h using only small volumes of blood components. Thus, compared to conventional methods, real-time PCR using MNBs allows for rapid detection with high sensitivity using only a small volume of blood components. Nature Publishing Group UK 2022-06-01 /pmc/articles/PMC9160056/ /pubmed/35650226 http://dx.doi.org/10.1038/s41598-022-12960-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Lee, Jinyeop Abafogi, Abdurhaman Teyib Oh, Sujin Chang, Ho Eun Tepeng, Wu Lee, Daekyu Park, Sungsu Park, Kyoung Un Hong, Yun Ji Molecular detection of bacterial contamination in plasma using magnetic-based enrichment |
title | Molecular detection of bacterial contamination in plasma using magnetic-based enrichment |
title_full | Molecular detection of bacterial contamination in plasma using magnetic-based enrichment |
title_fullStr | Molecular detection of bacterial contamination in plasma using magnetic-based enrichment |
title_full_unstemmed | Molecular detection of bacterial contamination in plasma using magnetic-based enrichment |
title_short | Molecular detection of bacterial contamination in plasma using magnetic-based enrichment |
title_sort | molecular detection of bacterial contamination in plasma using magnetic-based enrichment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160056/ https://www.ncbi.nlm.nih.gov/pubmed/35650226 http://dx.doi.org/10.1038/s41598-022-12960-5 |
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