Cargando…

Cell radiolabeling with acoustophoresis cell washing

Labeling immune cells with zirconium-89 ((89)Zr)-oxine has become a viable method to track cells in vivo by PET in various pre-clinical animal models and in clinical applications. Currently, (89)Zr-oxine cell labeling is performed manually, which requires a highly trained specialist and is prone to...

Descripción completa

Detalles Bibliográficos
Autores principales: Adler, Stephen S., Nyong, Emmanuel C., Glabman, Raisa A., Choyke, Peter L., Sato, Noriko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160075/
https://www.ncbi.nlm.nih.gov/pubmed/35650265
http://dx.doi.org/10.1038/s41598-022-13144-x
_version_ 1784719194560921600
author Adler, Stephen S.
Nyong, Emmanuel C.
Glabman, Raisa A.
Choyke, Peter L.
Sato, Noriko
author_facet Adler, Stephen S.
Nyong, Emmanuel C.
Glabman, Raisa A.
Choyke, Peter L.
Sato, Noriko
author_sort Adler, Stephen S.
collection PubMed
description Labeling immune cells with zirconium-89 ((89)Zr)-oxine has become a viable method to track cells in vivo by PET in various pre-clinical animal models and in clinical applications. Currently, (89)Zr-oxine cell labeling is performed manually, which requires a highly trained specialist and is prone to human error. As the first phase in developing a fully automated radiolabeling system to address this problem, we assess the use of acoustophoresis cell washing to replace the centrifugal cell washing used in the current (89)Zr-oxine cell radiolabeling procedure. To accomplish this, a cell radiolabeling procedure was developed in which two steps requiring a centrifuge to wash cells were replaced using acoustophoresis cell washing methods. The process was tested using murine EL4 lymphoma and T cells. The centrifuge cell labeling procedure was used as a control to compare the acoustophoresis cell washing procedure. The acoustophoresis method produced radiolabeled cells with similar properties to the centrifugal method when comparing labeling efficiency, labeled specific activity, efficacy of removing unbound (89)Zr-oxine from the suspension, cell viability measured using annexin V/propidium iodide staining and activation function. This suggests that acoustophoresis cell washing can be used in the design of an automated benchtop, good manufacture practice-qualified acoustophoresis cell radiolabeling device.
format Online
Article
Text
id pubmed-9160075
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-91600752022-06-03 Cell radiolabeling with acoustophoresis cell washing Adler, Stephen S. Nyong, Emmanuel C. Glabman, Raisa A. Choyke, Peter L. Sato, Noriko Sci Rep Article Labeling immune cells with zirconium-89 ((89)Zr)-oxine has become a viable method to track cells in vivo by PET in various pre-clinical animal models and in clinical applications. Currently, (89)Zr-oxine cell labeling is performed manually, which requires a highly trained specialist and is prone to human error. As the first phase in developing a fully automated radiolabeling system to address this problem, we assess the use of acoustophoresis cell washing to replace the centrifugal cell washing used in the current (89)Zr-oxine cell radiolabeling procedure. To accomplish this, a cell radiolabeling procedure was developed in which two steps requiring a centrifuge to wash cells were replaced using acoustophoresis cell washing methods. The process was tested using murine EL4 lymphoma and T cells. The centrifuge cell labeling procedure was used as a control to compare the acoustophoresis cell washing procedure. The acoustophoresis method produced radiolabeled cells with similar properties to the centrifugal method when comparing labeling efficiency, labeled specific activity, efficacy of removing unbound (89)Zr-oxine from the suspension, cell viability measured using annexin V/propidium iodide staining and activation function. This suggests that acoustophoresis cell washing can be used in the design of an automated benchtop, good manufacture practice-qualified acoustophoresis cell radiolabeling device. Nature Publishing Group UK 2022-06-01 /pmc/articles/PMC9160075/ /pubmed/35650265 http://dx.doi.org/10.1038/s41598-022-13144-x Text en © This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Adler, Stephen S.
Nyong, Emmanuel C.
Glabman, Raisa A.
Choyke, Peter L.
Sato, Noriko
Cell radiolabeling with acoustophoresis cell washing
title Cell radiolabeling with acoustophoresis cell washing
title_full Cell radiolabeling with acoustophoresis cell washing
title_fullStr Cell radiolabeling with acoustophoresis cell washing
title_full_unstemmed Cell radiolabeling with acoustophoresis cell washing
title_short Cell radiolabeling with acoustophoresis cell washing
title_sort cell radiolabeling with acoustophoresis cell washing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160075/
https://www.ncbi.nlm.nih.gov/pubmed/35650265
http://dx.doi.org/10.1038/s41598-022-13144-x
work_keys_str_mv AT adlerstephens cellradiolabelingwithacoustophoresiscellwashing
AT nyongemmanuelc cellradiolabelingwithacoustophoresiscellwashing
AT glabmanraisaa cellradiolabelingwithacoustophoresiscellwashing
AT choykepeterl cellradiolabelingwithacoustophoresiscellwashing
AT satonoriko cellradiolabelingwithacoustophoresiscellwashing