Extra-mitochondrial citrate synthase controls cAMP-dependent pathway during sperm acrosome reaction in mice

The sperm consumes adenosine triphosphate (ATP) to maintain the cellular function, viability, acrosome reaction (AR), and motility. Extra-mitochondrial citrate synthase (eCS) catalyzes citrate production in the sperm head, and thus regulates sperm function through ATP synthesis, similarly to CS. Thi...

Descripción completa

Detalles Bibliográficos
Autores principales: Kang, Woojin, Katano, Daiki, Kawano, Natsuko, Miyado, Mami, Miyado, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160978/
https://www.ncbi.nlm.nih.gov/pubmed/35663411
http://dx.doi.org/10.17912/micropub.biology.000579
_version_ 1784719386512195584
author Kang, Woojin
Katano, Daiki
Kawano, Natsuko
Miyado, Mami
Miyado, Kenji
author_facet Kang, Woojin
Katano, Daiki
Kawano, Natsuko
Miyado, Mami
Miyado, Kenji
author_sort Kang, Woojin
collection PubMed
description The sperm consumes adenosine triphosphate (ATP) to maintain the cellular function, viability, acrosome reaction (AR), and motility. Extra-mitochondrial citrate synthase (eCS) catalyzes citrate production in the sperm head, and thus regulates sperm function through ATP synthesis, similarly to CS. This study aimed to investigate how eCS regulates AR. Herein, acrosome-reacted (ARed) sperms were rarely detected on the zona pellucida, and spontaneous ARed sperm in eCs -deficient (KO) sperm remained at low levels even with induced capacitation. Retarded AR of eCs -KO sperm was enhanced by cyclic adenosine 3′,5′-monophosphate (cAMP) treatment. In conclusion, eCS regulates AR via a cAMP-dependent pathway, which presumably contributes to sperm metabolism.
format Online
Article
Text
id pubmed-9160978
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Caltech Library
record_format MEDLINE/PubMed
spelling pubmed-91609782022-06-03 Extra-mitochondrial citrate synthase controls cAMP-dependent pathway during sperm acrosome reaction in mice Kang, Woojin Katano, Daiki Kawano, Natsuko Miyado, Mami Miyado, Kenji MicroPubl Biol New Finding The sperm consumes adenosine triphosphate (ATP) to maintain the cellular function, viability, acrosome reaction (AR), and motility. Extra-mitochondrial citrate synthase (eCS) catalyzes citrate production in the sperm head, and thus regulates sperm function through ATP synthesis, similarly to CS. This study aimed to investigate how eCS regulates AR. Herein, acrosome-reacted (ARed) sperms were rarely detected on the zona pellucida, and spontaneous ARed sperm in eCs -deficient (KO) sperm remained at low levels even with induced capacitation. Retarded AR of eCs -KO sperm was enhanced by cyclic adenosine 3′,5′-monophosphate (cAMP) treatment. In conclusion, eCS regulates AR via a cAMP-dependent pathway, which presumably contributes to sperm metabolism. Caltech Library 2022-06-01 /pmc/articles/PMC9160978/ /pubmed/35663411 http://dx.doi.org/10.17912/micropub.biology.000579 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Kang, Woojin
Katano, Daiki
Kawano, Natsuko
Miyado, Mami
Miyado, Kenji
Extra-mitochondrial citrate synthase controls cAMP-dependent pathway during sperm acrosome reaction in mice
title Extra-mitochondrial citrate synthase controls cAMP-dependent pathway during sperm acrosome reaction in mice
title_full Extra-mitochondrial citrate synthase controls cAMP-dependent pathway during sperm acrosome reaction in mice
title_fullStr Extra-mitochondrial citrate synthase controls cAMP-dependent pathway during sperm acrosome reaction in mice
title_full_unstemmed Extra-mitochondrial citrate synthase controls cAMP-dependent pathway during sperm acrosome reaction in mice
title_short Extra-mitochondrial citrate synthase controls cAMP-dependent pathway during sperm acrosome reaction in mice
title_sort extra-mitochondrial citrate synthase controls camp-dependent pathway during sperm acrosome reaction in mice
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160978/
https://www.ncbi.nlm.nih.gov/pubmed/35663411
http://dx.doi.org/10.17912/micropub.biology.000579
work_keys_str_mv AT kangwoojin extramitochondrialcitratesynthasecontrolscampdependentpathwayduringspermacrosomereactioninmice
AT katanodaiki extramitochondrialcitratesynthasecontrolscampdependentpathwayduringspermacrosomereactioninmice
AT kawanonatsuko extramitochondrialcitratesynthasecontrolscampdependentpathwayduringspermacrosomereactioninmice
AT miyadomami extramitochondrialcitratesynthasecontrolscampdependentpathwayduringspermacrosomereactioninmice
AT miyadokenji extramitochondrialcitratesynthasecontrolscampdependentpathwayduringspermacrosomereactioninmice

Ejemplares similares