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Protective effect and mechanism of Shenkang injection on adenine-induced chronic renal failure in rats

PURPOSE: To investigate the protective effects of Shenkang injection (SKI) on adenine-induced chronic renal failure (CRF) in rat. METHODS: Sprague Dawley rats were randomly divided into five groups: control, model, and SKI groups (5, 10, 20 mL/kg). Rats in model and SKI groups were treated with aden...

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Autores principales: Chen, Rongchang, Xu, Lijiao, Zhang, Xu, Sun, Guibo, Zeng, Wenying, Sun, Xiaobo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161622/
https://www.ncbi.nlm.nih.gov/pubmed/35674582
http://dx.doi.org/10.1590/acb370304
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author Chen, Rongchang
Xu, Lijiao
Zhang, Xu
Sun, Guibo
Zeng, Wenying
Sun, Xiaobo
author_facet Chen, Rongchang
Xu, Lijiao
Zhang, Xu
Sun, Guibo
Zeng, Wenying
Sun, Xiaobo
author_sort Chen, Rongchang
collection PubMed
description PURPOSE: To investigate the protective effects of Shenkang injection (SKI) on adenine-induced chronic renal failure (CRF) in rat. METHODS: Sprague Dawley rats were randomly divided into five groups: control, model, and SKI groups (5, 10, 20 mL/kg). Rats in model and SKI groups were treated with adenine i.g. at a dose of 150 mg/kg every day for 12 weeks to induce CRF. Twelve weeks later, SKI was administered to the rat i.p. for four weeks. The effects of SKI on kidney injury and fibrosis were detected. RESULTS: SKI inhibited the elevation of the urine level of N-acetyl-b-D-glucosaminidase, kidney injury molecule-1, beta-2-microglobulin, urea protein in CRF rats. The serum levels of uric acid and serum creatinine increased and albumin decreased in the model group, which was prevented by SKI. SKI inhibited the release of inflammatory cytokines and increasing the activities of antioxidant enzymes in serum. SKI inhibited the expression of transforming growth factor-β1, vascular cell adhesion molecule 1, intercellular adhesion molecule 1, collagen I, collagen III, endothelin-1, laminin in kidney of CRF rats. CONCLUSIONS: SKI protected against adenine-induced kidney injury and fibrosis and exerted anti-inflammatory, and antioxidant effects in CRF rats.
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spelling pubmed-91616222022-06-16 Protective effect and mechanism of Shenkang injection on adenine-induced chronic renal failure in rats Chen, Rongchang Xu, Lijiao Zhang, Xu Sun, Guibo Zeng, Wenying Sun, Xiaobo Acta Cir Bras Original Article PURPOSE: To investigate the protective effects of Shenkang injection (SKI) on adenine-induced chronic renal failure (CRF) in rat. METHODS: Sprague Dawley rats were randomly divided into five groups: control, model, and SKI groups (5, 10, 20 mL/kg). Rats in model and SKI groups were treated with adenine i.g. at a dose of 150 mg/kg every day for 12 weeks to induce CRF. Twelve weeks later, SKI was administered to the rat i.p. for four weeks. The effects of SKI on kidney injury and fibrosis were detected. RESULTS: SKI inhibited the elevation of the urine level of N-acetyl-b-D-glucosaminidase, kidney injury molecule-1, beta-2-microglobulin, urea protein in CRF rats. The serum levels of uric acid and serum creatinine increased and albumin decreased in the model group, which was prevented by SKI. SKI inhibited the release of inflammatory cytokines and increasing the activities of antioxidant enzymes in serum. SKI inhibited the expression of transforming growth factor-β1, vascular cell adhesion molecule 1, intercellular adhesion molecule 1, collagen I, collagen III, endothelin-1, laminin in kidney of CRF rats. CONCLUSIONS: SKI protected against adenine-induced kidney injury and fibrosis and exerted anti-inflammatory, and antioxidant effects in CRF rats. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2022-06-01 /pmc/articles/PMC9161622/ /pubmed/35674582 http://dx.doi.org/10.1590/acb370304 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Chen, Rongchang
Xu, Lijiao
Zhang, Xu
Sun, Guibo
Zeng, Wenying
Sun, Xiaobo
Protective effect and mechanism of Shenkang injection on adenine-induced chronic renal failure in rats
title Protective effect and mechanism of Shenkang injection on adenine-induced chronic renal failure in rats
title_full Protective effect and mechanism of Shenkang injection on adenine-induced chronic renal failure in rats
title_fullStr Protective effect and mechanism of Shenkang injection on adenine-induced chronic renal failure in rats
title_full_unstemmed Protective effect and mechanism of Shenkang injection on adenine-induced chronic renal failure in rats
title_short Protective effect and mechanism of Shenkang injection on adenine-induced chronic renal failure in rats
title_sort protective effect and mechanism of shenkang injection on adenine-induced chronic renal failure in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161622/
https://www.ncbi.nlm.nih.gov/pubmed/35674582
http://dx.doi.org/10.1590/acb370304
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