High expression of H2A histone family member Y promotes the proliferation and autophagy of hepatocellular carcinoma cells

Hepatocellular carcinoma is a common malignant tumor and the third most common cause of cancer-related deaths. In this study, we selected H2AFY as a potential oncogene from three online databases, and verified differential expression between normal and liver cancer tissues. Moreover, H2AFY expressio...

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Autores principales: Lei, Jiasheng, Ma, Shuoshuo, Sun, Wanliang, Wang, Dongdong, Lu, Zheng, Zhang, Dengyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161916/
https://www.ncbi.nlm.nih.gov/pubmed/35472308
http://dx.doi.org/10.1080/21655979.2022.2065761
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author Lei, Jiasheng
Ma, Shuoshuo
Sun, Wanliang
Wang, Dongdong
Lu, Zheng
Zhang, Dengyong
author_facet Lei, Jiasheng
Ma, Shuoshuo
Sun, Wanliang
Wang, Dongdong
Lu, Zheng
Zhang, Dengyong
author_sort Lei, Jiasheng
collection PubMed
description Hepatocellular carcinoma is a common malignant tumor and the third most common cause of cancer-related deaths. In this study, we selected H2AFY as a potential oncogene from three online databases, and verified differential expression between normal and liver cancer tissues. Moreover, H2AFY expression was significantly correlated with the clinical characteristics and the survival of liver cancer patients. H2AFY expression was correlated with poor prognosis of liver cancer patients. H2AFY expression was also significantly higher in liver cancer cells. Knockdown and overexpression of H2AFY in liver cancer cells showed that H2AFY promoted the proliferation and clone formation of liver cancer cells but had no significant effects on the migration and invasion ability of liver cancer cells. Western blot analysis, immunohistochemistry, and immunofluorescence double staining confirmed that H2AFY upregulated LC3 and p62 expression in liver cancer tissues and cells. In conclusion, H2AFY is highly expressed in liver cancer cells and tissues, and promotes the proliferation and autophagy of liver cancer cells. H2AFY is a potential target for liver cancer therapy. Abbreviations: APLF: aprataxin pnk-like factor; HCC: Hepatocellular carcinoma; H2AFY: H2A histone family member Y
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spelling pubmed-91619162022-06-03 High expression of H2A histone family member Y promotes the proliferation and autophagy of hepatocellular carcinoma cells Lei, Jiasheng Ma, Shuoshuo Sun, Wanliang Wang, Dongdong Lu, Zheng Zhang, Dengyong Bioengineered Research Paper Hepatocellular carcinoma is a common malignant tumor and the third most common cause of cancer-related deaths. In this study, we selected H2AFY as a potential oncogene from three online databases, and verified differential expression between normal and liver cancer tissues. Moreover, H2AFY expression was significantly correlated with the clinical characteristics and the survival of liver cancer patients. H2AFY expression was correlated with poor prognosis of liver cancer patients. H2AFY expression was also significantly higher in liver cancer cells. Knockdown and overexpression of H2AFY in liver cancer cells showed that H2AFY promoted the proliferation and clone formation of liver cancer cells but had no significant effects on the migration and invasion ability of liver cancer cells. Western blot analysis, immunohistochemistry, and immunofluorescence double staining confirmed that H2AFY upregulated LC3 and p62 expression in liver cancer tissues and cells. In conclusion, H2AFY is highly expressed in liver cancer cells and tissues, and promotes the proliferation and autophagy of liver cancer cells. H2AFY is a potential target for liver cancer therapy. Abbreviations: APLF: aprataxin pnk-like factor; HCC: Hepatocellular carcinoma; H2AFY: H2A histone family member Y Taylor & Francis 2022-04-26 /pmc/articles/PMC9161916/ /pubmed/35472308 http://dx.doi.org/10.1080/21655979.2022.2065761 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Lei, Jiasheng
Ma, Shuoshuo
Sun, Wanliang
Wang, Dongdong
Lu, Zheng
Zhang, Dengyong
High expression of H2A histone family member Y promotes the proliferation and autophagy of hepatocellular carcinoma cells
title High expression of H2A histone family member Y promotes the proliferation and autophagy of hepatocellular carcinoma cells
title_full High expression of H2A histone family member Y promotes the proliferation and autophagy of hepatocellular carcinoma cells
title_fullStr High expression of H2A histone family member Y promotes the proliferation and autophagy of hepatocellular carcinoma cells
title_full_unstemmed High expression of H2A histone family member Y promotes the proliferation and autophagy of hepatocellular carcinoma cells
title_short High expression of H2A histone family member Y promotes the proliferation and autophagy of hepatocellular carcinoma cells
title_sort high expression of h2a histone family member y promotes the proliferation and autophagy of hepatocellular carcinoma cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161916/
https://www.ncbi.nlm.nih.gov/pubmed/35472308
http://dx.doi.org/10.1080/21655979.2022.2065761
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