The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer

Double homeobox A pseudogene 8 (DUXAP8) is a known tumor promoter in several malignancies. Nonetheless, its function in colon cancer (CC) is indefinite. Herein, we explored the significance of DUXAP8 and its underlying mechanism in CC. Our data indicated that DUXAP8 was upregulated in CC, and it was...

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Autores principales: Mao, Shengxun, Mo, Zhaohong, Wu, Runxin, Lai, Bin, Zhou, Zhiyong, Song, Yi, Ouyang, Xi, Zhu, Xingen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161926/
https://www.ncbi.nlm.nih.gov/pubmed/35287542
http://dx.doi.org/10.1080/21655979.2022.2053802
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author Mao, Shengxun
Mo, Zhaohong
Wu, Runxin
Lai, Bin
Zhou, Zhiyong
Song, Yi
Ouyang, Xi
Zhu, Xingen
author_facet Mao, Shengxun
Mo, Zhaohong
Wu, Runxin
Lai, Bin
Zhou, Zhiyong
Song, Yi
Ouyang, Xi
Zhu, Xingen
author_sort Mao, Shengxun
collection PubMed
description Double homeobox A pseudogene 8 (DUXAP8) is a known tumor promoter in several malignancies. Nonetheless, its function in colon cancer (CC) is indefinite. Herein, we explored the significance of DUXAP8 and its underlying mechanism in CC. Our data indicated that DUXAP8 was upregulated in CC, and it was related to advanced stages and lymph node metastases. Based on our Kaplan-Meier survival analysis, elevated DUXAP8 expression resulted in shorter patient overall survival (OS). Conversely, DUXAP8 silencing strongly suppressed cellular proliferation, migration and invasion in vitro. Based on our western blot analysis, DUXAP8 deficiency strongly inhibited the epithelial–mesenchymal transition (EMT) in vitro. Alternately, DUXAP8 overexpression accelerated cellular proliferation migration and invasion in CC. Finally, silencing DUXAP8 prevented tumorigenesis in a mouse xenograft model in vivo. Collectively, our results demonstrated that DUXAP8 regulates the occurrence and advancement of CC, and may serve as a regulatory hub for this disease.
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spelling pubmed-91619262022-06-03 The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer Mao, Shengxun Mo, Zhaohong Wu, Runxin Lai, Bin Zhou, Zhiyong Song, Yi Ouyang, Xi Zhu, Xingen Bioengineered Research Paper Double homeobox A pseudogene 8 (DUXAP8) is a known tumor promoter in several malignancies. Nonetheless, its function in colon cancer (CC) is indefinite. Herein, we explored the significance of DUXAP8 and its underlying mechanism in CC. Our data indicated that DUXAP8 was upregulated in CC, and it was related to advanced stages and lymph node metastases. Based on our Kaplan-Meier survival analysis, elevated DUXAP8 expression resulted in shorter patient overall survival (OS). Conversely, DUXAP8 silencing strongly suppressed cellular proliferation, migration and invasion in vitro. Based on our western blot analysis, DUXAP8 deficiency strongly inhibited the epithelial–mesenchymal transition (EMT) in vitro. Alternately, DUXAP8 overexpression accelerated cellular proliferation migration and invasion in CC. Finally, silencing DUXAP8 prevented tumorigenesis in a mouse xenograft model in vivo. Collectively, our results demonstrated that DUXAP8 regulates the occurrence and advancement of CC, and may serve as a regulatory hub for this disease. Taylor & Francis 2022-03-24 /pmc/articles/PMC9161926/ /pubmed/35287542 http://dx.doi.org/10.1080/21655979.2022.2053802 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Mao, Shengxun
Mo, Zhaohong
Wu, Runxin
Lai, Bin
Zhou, Zhiyong
Song, Yi
Ouyang, Xi
Zhu, Xingen
The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer
title The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer
title_full The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer
title_fullStr The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer
title_full_unstemmed The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer
title_short The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer
title_sort double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161926/
https://www.ncbi.nlm.nih.gov/pubmed/35287542
http://dx.doi.org/10.1080/21655979.2022.2053802
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