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Effects of silencing neuropilin-2 on proliferation, migration, and invasion of colorectal cancer HT-29

To investigate the effects of silencing neuropilin-2(NRP-2) on the proliferation, migration, and invasion of colorectal cancer(CRC) HT-29. Lipofectamine 2000 was used to transfect specific siRNA for NRP-2 and nonspecific control siRNA into human colorectal cancer HT-29 as the transfection group and...

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Autores principales: Zhan, Qi-Qi, Liu, Qian-Yu, Yang, Xin, Ge, Yu-Hui, Xu, Lei, Ding, Guan-Yi, Guo, Shang, Zhu, Bing, Xu, Wei-Guo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161945/
https://www.ncbi.nlm.nih.gov/pubmed/35473507
http://dx.doi.org/10.1080/21655979.2022.2068363
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author Zhan, Qi-Qi
Liu, Qian-Yu
Yang, Xin
Ge, Yu-Hui
Xu, Lei
Ding, Guan-Yi
Guo, Shang
Zhu, Bing
Xu, Wei-Guo
author_facet Zhan, Qi-Qi
Liu, Qian-Yu
Yang, Xin
Ge, Yu-Hui
Xu, Lei
Ding, Guan-Yi
Guo, Shang
Zhu, Bing
Xu, Wei-Guo
author_sort Zhan, Qi-Qi
collection PubMed
description To investigate the effects of silencing neuropilin-2(NRP-2) on the proliferation, migration, and invasion of colorectal cancer(CRC) HT-29. Lipofectamine 2000 was used to transfect specific siRNA for NRP-2 and nonspecific control siRNA into human colorectal cancer HT-29 as the transfection group and meaningless sequence group. HT-29 cultured in a medium was used as the blank control group. The expression levels of NRP-2 mRNA in the cells were detected by real-time fluorescence quantitative PCR. The expressions of proliferation-associated protein Ki-67 in the cells were detected by immunochemical staining. Migration ability was assessed by a monolayer cell scratch wound damage and repair experiment. The Transwell chamber invasion experiment was adopted to determine invasive ability by measuring the number of tumor cells crossing the chamber membrane. Compared with the meaningless sequence group and blank control group, real-time fluorescence quantitative PCR showed that the relative expression level of NRP-2 mRNA in the transfection group was significantly decreased(P < 0.05). Results of immunochemical staining revealed that the expression of Ki-67 protein in the transfected cells was significantly reduced, and the proliferation ability was decreased(P < 0.05). The results further showed that the scratch healing rate of the transfected cells decreased after 24 h of healing(P < 0.05). Results of Transwell invasion assay showed that the number of cells passing through the stromal membrane of the upper chamber to the back of the chamber was significantly reduced in the transfection group(p < 0.05). Silencing NRP-2 could inhibit the proliferation, migration, and invasion of colorectal cancer HT-29.
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spelling pubmed-91619452022-06-03 Effects of silencing neuropilin-2 on proliferation, migration, and invasion of colorectal cancer HT-29 Zhan, Qi-Qi Liu, Qian-Yu Yang, Xin Ge, Yu-Hui Xu, Lei Ding, Guan-Yi Guo, Shang Zhu, Bing Xu, Wei-Guo Bioengineered Research Paper To investigate the effects of silencing neuropilin-2(NRP-2) on the proliferation, migration, and invasion of colorectal cancer(CRC) HT-29. Lipofectamine 2000 was used to transfect specific siRNA for NRP-2 and nonspecific control siRNA into human colorectal cancer HT-29 as the transfection group and meaningless sequence group. HT-29 cultured in a medium was used as the blank control group. The expression levels of NRP-2 mRNA in the cells were detected by real-time fluorescence quantitative PCR. The expressions of proliferation-associated protein Ki-67 in the cells were detected by immunochemical staining. Migration ability was assessed by a monolayer cell scratch wound damage and repair experiment. The Transwell chamber invasion experiment was adopted to determine invasive ability by measuring the number of tumor cells crossing the chamber membrane. Compared with the meaningless sequence group and blank control group, real-time fluorescence quantitative PCR showed that the relative expression level of NRP-2 mRNA in the transfection group was significantly decreased(P < 0.05). Results of immunochemical staining revealed that the expression of Ki-67 protein in the transfected cells was significantly reduced, and the proliferation ability was decreased(P < 0.05). The results further showed that the scratch healing rate of the transfected cells decreased after 24 h of healing(P < 0.05). Results of Transwell invasion assay showed that the number of cells passing through the stromal membrane of the upper chamber to the back of the chamber was significantly reduced in the transfection group(p < 0.05). Silencing NRP-2 could inhibit the proliferation, migration, and invasion of colorectal cancer HT-29. Taylor & Francis 2022-04-27 /pmc/articles/PMC9161945/ /pubmed/35473507 http://dx.doi.org/10.1080/21655979.2022.2068363 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Zhan, Qi-Qi
Liu, Qian-Yu
Yang, Xin
Ge, Yu-Hui
Xu, Lei
Ding, Guan-Yi
Guo, Shang
Zhu, Bing
Xu, Wei-Guo
Effects of silencing neuropilin-2 on proliferation, migration, and invasion of colorectal cancer HT-29
title Effects of silencing neuropilin-2 on proliferation, migration, and invasion of colorectal cancer HT-29
title_full Effects of silencing neuropilin-2 on proliferation, migration, and invasion of colorectal cancer HT-29
title_fullStr Effects of silencing neuropilin-2 on proliferation, migration, and invasion of colorectal cancer HT-29
title_full_unstemmed Effects of silencing neuropilin-2 on proliferation, migration, and invasion of colorectal cancer HT-29
title_short Effects of silencing neuropilin-2 on proliferation, migration, and invasion of colorectal cancer HT-29
title_sort effects of silencing neuropilin-2 on proliferation, migration, and invasion of colorectal cancer ht-29
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161945/
https://www.ncbi.nlm.nih.gov/pubmed/35473507
http://dx.doi.org/10.1080/21655979.2022.2068363
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