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Chrysin reduces inflammation and oxidative stress and improves ovarian function in D-gal-induced premature ovarian failure
Premature ovarian failure (POF), a frequently occurring pathology. Chrysin has antioxidant, anti-inflammatory, anti-apoptotic and other pharmacological activities. This study was designed to detect the effect of Chrysin on POF. The establishment of POF was depended on the subcutaneous injection of D...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161991/ https://www.ncbi.nlm.nih.gov/pubmed/35311454 http://dx.doi.org/10.1080/21655979.2021.2005991 |
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author | Li, Xinning Li, Xuelan Deng, Ling |
author_facet | Li, Xinning Li, Xuelan Deng, Ling |
author_sort | Li, Xinning |
collection | PubMed |
description | Premature ovarian failure (POF), a frequently occurring pathology. Chrysin has antioxidant, anti-inflammatory, anti-apoptotic and other pharmacological activities. This study was designed to detect the effect of Chrysin on POF. The establishment of POF was depended on the subcutaneous injection of D-gal (200 mg/kg/d). With the adoption of ELISA, the levels of hormones and release of inflammatory cytokines were assayed. The expression of MDA, GSH-px, SOD and ROS was evaluated with corresponding kits. In addition, the pathological changes of ovary and apoptosis of ovarian granulosa cells in D-gal-induced mice were detected using H&E staining and TUNEL, respectively. Moreover, the levels of FSH receptor and apoptosis-related proteins were measured with western blot. Finally, ERβ expression was measured with RT-qPCR and western blot. In this study, we found that chrysin regulated the expression of hormones and weight of D-gal-induced mice. It was also found that chrysin inhibited the inflammation and oxidative stress in mice with D-gal induction. In addition, the number and advancement of follicle in D-gal-induced mice treated with chrysin revealed that chrysin could improve the ovarian function of mice with POF. Furthermore, chrysin exhibited inhibitory effects on the apoptosis of ovarian granulosa cells in D-gal-induced mice. More importantly, chrysin molecule targeted ERβ and activated ERβ expression in POF. Overall, Chrysin reduces inflammation and oxidative stress and improves ovarian function in D-gal-induced premature ovarian failure, suggesting that chrysin is valuable for the treatment of POF. |
format | Online Article Text |
id | pubmed-9161991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-91619912022-06-03 Chrysin reduces inflammation and oxidative stress and improves ovarian function in D-gal-induced premature ovarian failure Li, Xinning Li, Xuelan Deng, Ling Bioengineered Research Paper Premature ovarian failure (POF), a frequently occurring pathology. Chrysin has antioxidant, anti-inflammatory, anti-apoptotic and other pharmacological activities. This study was designed to detect the effect of Chrysin on POF. The establishment of POF was depended on the subcutaneous injection of D-gal (200 mg/kg/d). With the adoption of ELISA, the levels of hormones and release of inflammatory cytokines were assayed. The expression of MDA, GSH-px, SOD and ROS was evaluated with corresponding kits. In addition, the pathological changes of ovary and apoptosis of ovarian granulosa cells in D-gal-induced mice were detected using H&E staining and TUNEL, respectively. Moreover, the levels of FSH receptor and apoptosis-related proteins were measured with western blot. Finally, ERβ expression was measured with RT-qPCR and western blot. In this study, we found that chrysin regulated the expression of hormones and weight of D-gal-induced mice. It was also found that chrysin inhibited the inflammation and oxidative stress in mice with D-gal induction. In addition, the number and advancement of follicle in D-gal-induced mice treated with chrysin revealed that chrysin could improve the ovarian function of mice with POF. Furthermore, chrysin exhibited inhibitory effects on the apoptosis of ovarian granulosa cells in D-gal-induced mice. More importantly, chrysin molecule targeted ERβ and activated ERβ expression in POF. Overall, Chrysin reduces inflammation and oxidative stress and improves ovarian function in D-gal-induced premature ovarian failure, suggesting that chrysin is valuable for the treatment of POF. Taylor & Francis 2022-03-24 /pmc/articles/PMC9161991/ /pubmed/35311454 http://dx.doi.org/10.1080/21655979.2021.2005991 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Li, Xinning Li, Xuelan Deng, Ling Chrysin reduces inflammation and oxidative stress and improves ovarian function in D-gal-induced premature ovarian failure |
title | Chrysin reduces inflammation and oxidative stress and improves ovarian function in D-gal-induced premature ovarian failure |
title_full | Chrysin reduces inflammation and oxidative stress and improves ovarian function in D-gal-induced premature ovarian failure |
title_fullStr | Chrysin reduces inflammation and oxidative stress and improves ovarian function in D-gal-induced premature ovarian failure |
title_full_unstemmed | Chrysin reduces inflammation and oxidative stress and improves ovarian function in D-gal-induced premature ovarian failure |
title_short | Chrysin reduces inflammation and oxidative stress and improves ovarian function in D-gal-induced premature ovarian failure |
title_sort | chrysin reduces inflammation and oxidative stress and improves ovarian function in d-gal-induced premature ovarian failure |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161991/ https://www.ncbi.nlm.nih.gov/pubmed/35311454 http://dx.doi.org/10.1080/21655979.2021.2005991 |
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