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Hydrogen Sulfide Diminishes Activation of Adventitial Fibroblasts Through the Inhibition of Mitochondrial Fission

Activation of adventitial fibroblasts (AFs) on vascular injury contributes to vascular remodeling. Hydrogen sulfide (H(2)S), a gaseous signal molecule, modulates various cardiovascular functions. The aim of this study was to explore whether exogenous H(2)S ameliorates transforming growth factor-β1 (...

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Autores principales: Lu, Zhao-Yang, Guo, Chun-Ling, Yang, Bin, Yao, Yao, Yang, Zhuo-Jing, Gong, Yu-Xin, Yang, Jing-Yao, Dong, Wen-Yuan, Yang, Jun, Yang, Hai-Bing, Liu, Hui-Min, Li, Bao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Cardiovascular Pharmacology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162271/
https://www.ncbi.nlm.nih.gov/pubmed/35234738
http://dx.doi.org/10.1097/FJC.0000000000001250
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author Lu, Zhao-Yang
Guo, Chun-Ling
Yang, Bin
Yao, Yao
Yang, Zhuo-Jing
Gong, Yu-Xin
Yang, Jing-Yao
Dong, Wen-Yuan
Yang, Jun
Yang, Hai-Bing
Liu, Hui-Min
Li, Bao
author_facet Lu, Zhao-Yang
Guo, Chun-Ling
Yang, Bin
Yao, Yao
Yang, Zhuo-Jing
Gong, Yu-Xin
Yang, Jing-Yao
Dong, Wen-Yuan
Yang, Jun
Yang, Hai-Bing
Liu, Hui-Min
Li, Bao
author_sort Lu, Zhao-Yang
collection PubMed
description Activation of adventitial fibroblasts (AFs) on vascular injury contributes to vascular remodeling. Hydrogen sulfide (H(2)S), a gaseous signal molecule, modulates various cardiovascular functions. The aim of this study was to explore whether exogenous H(2)S ameliorates transforming growth factor-β1 (TGF-β1)–induced activation of AFs and, if so, to determine the underlying molecular mechanisms. Immunofluorescent staining and western blot were used to determine the expression of collagen I and α-smooth muscle actin. The proliferation and migration of AFs were performed by using cell counting Kit-8 and transwell assay, respectively. The mitochondrial morphology was assessed by using MitoTracker Red staining. The activation of signaling pathway was evaluated by western blot. The mitochondrial reactive oxygen species and mitochondrial membrane potential were determined by MitoSOX and JC-1 (5,5′,6,6′-tetrachloro-1,1,3,3′-tetraethylbenzimidazolyl carbocyanine iodide) staining. Our study demonstrated exogenous H(2)S treatment dramatically suppressed TGF-β1–induced AF proliferation, migration, and phenotypic transition by blockage of dynamin-related protein 1 (Drp1)–mediated mitochondrial fission and regulated mitochondrial reactive oxygen species generation. Moreover, exogenous H(2)S reversed TGF-β1–induced mitochondrial fission and AF activation by modulating Rho-associated protein kinase 1–dependent phosphorylation of Drp1. In conclusion, our results suggested that exogenous H(2)S attenuates TGF-β1–induced AF activation through suppression of Drp1-mediated mitochondrial fission in a Rho-associated protein kinase 1–dependent fashion.
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spelling pubmed-91622712022-06-08 Hydrogen Sulfide Diminishes Activation of Adventitial Fibroblasts Through the Inhibition of Mitochondrial Fission Lu, Zhao-Yang Guo, Chun-Ling Yang, Bin Yao, Yao Yang, Zhuo-Jing Gong, Yu-Xin Yang, Jing-Yao Dong, Wen-Yuan Yang, Jun Yang, Hai-Bing Liu, Hui-Min Li, Bao J Cardiovasc Pharmacol Original Article Activation of adventitial fibroblasts (AFs) on vascular injury contributes to vascular remodeling. Hydrogen sulfide (H(2)S), a gaseous signal molecule, modulates various cardiovascular functions. The aim of this study was to explore whether exogenous H(2)S ameliorates transforming growth factor-β1 (TGF-β1)–induced activation of AFs and, if so, to determine the underlying molecular mechanisms. Immunofluorescent staining and western blot were used to determine the expression of collagen I and α-smooth muscle actin. The proliferation and migration of AFs were performed by using cell counting Kit-8 and transwell assay, respectively. The mitochondrial morphology was assessed by using MitoTracker Red staining. The activation of signaling pathway was evaluated by western blot. The mitochondrial reactive oxygen species and mitochondrial membrane potential were determined by MitoSOX and JC-1 (5,5′,6,6′-tetrachloro-1,1,3,3′-tetraethylbenzimidazolyl carbocyanine iodide) staining. Our study demonstrated exogenous H(2)S treatment dramatically suppressed TGF-β1–induced AF proliferation, migration, and phenotypic transition by blockage of dynamin-related protein 1 (Drp1)–mediated mitochondrial fission and regulated mitochondrial reactive oxygen species generation. Moreover, exogenous H(2)S reversed TGF-β1–induced mitochondrial fission and AF activation by modulating Rho-associated protein kinase 1–dependent phosphorylation of Drp1. In conclusion, our results suggested that exogenous H(2)S attenuates TGF-β1–induced AF activation through suppression of Drp1-mediated mitochondrial fission in a Rho-associated protein kinase 1–dependent fashion. Journal of Cardiovascular Pharmacology 2022-06 2022-03-02 /pmc/articles/PMC9162271/ /pubmed/35234738 http://dx.doi.org/10.1097/FJC.0000000000001250 Text en Copyright © 2022 The Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
spellingShingle Original Article
Lu, Zhao-Yang
Guo, Chun-Ling
Yang, Bin
Yao, Yao
Yang, Zhuo-Jing
Gong, Yu-Xin
Yang, Jing-Yao
Dong, Wen-Yuan
Yang, Jun
Yang, Hai-Bing
Liu, Hui-Min
Li, Bao
Hydrogen Sulfide Diminishes Activation of Adventitial Fibroblasts Through the Inhibition of Mitochondrial Fission
title Hydrogen Sulfide Diminishes Activation of Adventitial Fibroblasts Through the Inhibition of Mitochondrial Fission
title_full Hydrogen Sulfide Diminishes Activation of Adventitial Fibroblasts Through the Inhibition of Mitochondrial Fission
title_fullStr Hydrogen Sulfide Diminishes Activation of Adventitial Fibroblasts Through the Inhibition of Mitochondrial Fission
title_full_unstemmed Hydrogen Sulfide Diminishes Activation of Adventitial Fibroblasts Through the Inhibition of Mitochondrial Fission
title_short Hydrogen Sulfide Diminishes Activation of Adventitial Fibroblasts Through the Inhibition of Mitochondrial Fission
title_sort hydrogen sulfide diminishes activation of adventitial fibroblasts through the inhibition of mitochondrial fission
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162271/
https://www.ncbi.nlm.nih.gov/pubmed/35234738
http://dx.doi.org/10.1097/FJC.0000000000001250
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